Studies on the pleomorphism of HVJ virions

Hosaka, Yasuhiro; Kitano, H; Ikeguchi, Sakiko

doi:10.1016/0042-6822(66)90027-4

Cited by 95 publications

(36 citation statements)

References 16 publications

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“…The major peak indicated R264, whereas the minor one indicated the wt G264 (overlooked in our initial examination), suggesting that the P3-2 virus contained two types of genomes differing in the F gene. As paramyxoviruses are known to have the ability to include several genomes in one viral particle, called polyploidy [12][13][14] , our observation is not unexpected.…”

Section: Fusion Assay Inconsistent With One Recombinant Virussupporting

confidence: 55%

Cooperation between different RNA virus genomes produces a new phenotype

2012

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Section: Fusion Assay Inconsistent With One Recombinant Virussupporting

confidence: 55%

Cooperation between different RNA virus genomes produces a new phenotype

2012

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“…Most of the phenotypically mixed virions seemed to be of the bullet shape, typical for VSV, as shown in Fig. 3; however, considering the pleomorphism of HVJ virions [9], it may also be conceivable that there might be some spherical incomplete HVJ virions, which contain VSV plaque-forming genomes. Although this assumption seemed to be of interest to know the interaction of nucleocapsid and virus envelope in the process of assembly, this possibility could not be confirmed as yet.…”

Section: Sucrose Gradient Centrifugation Analysismentioning

confidence: 99%

Phenotypic Mixing of Vesicular Stomatitis Virus with HVJ (Sendai Virus)

Kimura¹

1973

Japanese Journal of Microbiology

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Vesicular stomatitis virus (VSV) plaque-forming virions obtained from mixed infection of HeLa cells with VSV and HVJ (Sendai virus) were shown to contain two types of phenotypically mixed virions : virions doubly neutralizable with both anti-VSV and anti-HVJ antisera (up to 15%), and virions which could be neutralized only with anti-HVJ serum and not with anti-VSV serum (up to 0.3%). No evidence for genetic recombination was obtained between these two viruses. Phenotypically mixed virions could adsorb to and elute from chiken erythrocytes, and bullet-shaped virions, typical of VSV, were found to be adsorbed to chicken erythrocytes in electron microscopy. Neuraminidase activity was detected in the virions specifically sedimented with anti-VSV serum. From these findings, phenotypically mixed virions were suggested to contain hemagglutinin and neuraminidase of HVJ in the envelope. In sucrose gradient centrifugation, phenotypically mixed virions enclosed within HVJ antigens sedimented slightly faster than standard HVJ.Simultaneous multiplication of two different viruses in cells can cause appearance of a progeny with varying characteristics. The occurrence of phenotypic mixing within the same group of viruses, such as myxoviruses [5,8] This paper describes evidence for the occurrence of phenotypic mixing between morphologically distinct groups of enveloped viruses, HVJ (Sendai virus) and VSV. Since HeLa cells produce only a few amount of HVJ progeny in a non-infectious form after a relatively long period of incubation, which is very expedient for investigation of phenotypic mixing, experiments have been performed mainly to clarify the characteristics of the VSV plaque-forming virions from mixed infection.

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“…Mumps The nucleocapsids of Sendai virus (Hosaka, Kitano & Ikeguchi, 1966), Newcastle disease virus (Compans & Choppin,~967a), SV 5 (Compans & Choppin,~967b) and mumps virus (Hosaka & Shimizu,I968 ) have all been shown to have a length of about r #m. and width of about I8 nm.…”

Section: Introductionmentioning

confidence: 99%

Observations on the Structure of the Nucleocapsids of some Paramyxoviruses

Finch

Gibbs

1970

Journal of General Virology

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SUMMARYThe intact particles and nucteocapsids of mumps, Sendai and measles viruses are of closely similar appearance, size and structure. The intact particles are about 15o nm. in diameter. The filamentous nucleocapsids have a modal length of about ~. I/Am., and are constructed of subunits arranged as a single start helix of pitch 5.0 nm. for Sendai virus, and about 6"o nm. for mumps and measles viruses. A (helical) projection of the structure of the Sendai nucleocapsid calculated from an electron micrograph showed that the structural subunits are hour-glass-shaped and are arranged in the helix with their long axes inclined at an angle of about 6o ° to the long axis of the particle. There are probably J ~ or I3 subunits in each turn of the basic helix. Optical diffraction patterns of electron micrographs of mumps and measles nucleocapsids show that they have closely similar structures.

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Studies on the pleomorphism of HVJ virions

Cited by 95 publications

References 16 publications

Cooperation between different RNA virus genomes produces a new phenotype

Cooperation between different RNA virus genomes produces a new phenotype

Phenotypic Mixing of Vesicular Stomatitis Virus with HVJ (Sendai Virus)

Observations on the Structure of the Nucleocapsids of some Paramyxoviruses

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