Studies on the prevalence of oncogenic HPV types among Lithuanian women with cervical pathology

Abstract

Human papillomavirus (HPV) is the main cause of cervical cancer. Therefore, the detection of oncogenic HPV types is important in predicting the risk of cervical cancer. The aim of the current study was to estimate the prevalence of 16 carcinogenic and potentially carcinogenic HPV types in the study group of Lithuanian women with various grades of cervical pathology in comparison to healthy women. A total of 824 cervical specimens were investigated for HPV DNA: 547 specimens of women with abnormal cytology and 277 specimens of healthy women. Cytological diagnosis was confirmed by histology. For the detection of HPV infection, HPV DNA was amplified by PCR using three different primer systems. HPV DNA was detected in 67.6% of specimens collected from women with abnormal cytology and 24.2% of specimens collected from healthy women. The frequency of HPV-positive specimens correlated with the severity of cervical pathology: it ranged from 50.0% in the subgroup of atypical squamous cells to 80.6% in cervical cancer. In cases confirmed by histology the frequency of HPV-positive specimens ranged from 68.6% in the subgroup of cervical intraepithelial neoplasia grade 1 to 89.2% in cervical intraepithelial neoplasia grade 3 or carcinoma in situ. HPV DNA-positive samples were further investigated for the presence of 16 HPV types by multiplex PCR. The most common HPV type was HPV 16 (detected in 42.3% of HPV-positive specimens) followed by HPV 31 (10.1%), HPV 33 (8.2%), and HPV 56 (5.7%). In contrast, the frequency of HPV 18 was lower as compared to other countries.

The Prevalence of Human Papillomavirus Genotypes in Women with Abnormal Cervical Cytology

“…Honduras'ta Tabore ve arkadaşları %88.2, Hindistan'da Baskaran ve arkadaşları %84.3, Fas'ta Assoumou ve arkadaşları %76.9, Kore'de Kim ve arkadaşları %75, Litvanya'da Simanaviciene ve arkadaşları %67.6, Venezuela'da Corenti ve arkadaşları %62, Romanya'da Corneanu ve arkadaşları %61, Çin'de Jiang ve arkadaşları %39.4 oranında bulmuşlardır. Ülkemizde Ankara'da yapılan çalışmalardan, Ergünay ve arkadaşları %80, Dursun ve arkadaşları %52, Aydoğan ve arkadaşları %50, Mersin'de yapılan çalışmada Tezcan ve arkadaşları %35.4 oranında tespit etmişlerdir [8][9][10][11][12][13][14][15][16][17][18][19] .…”

“…Assoumou ve arkadaşları LSIL sitolojili kadınlarda HR HPV prevalansını %87.5, Tabora ve arkadaşları %81, Corenti ve arkadaşları %68, Simanaviciene ve arkadaşları %55.2, Jiang ve arkadaşları %43.5 olarak bulmuşlardır. Ülkemizde ise Dursun ve arkadaşları LSIL sitolojili kadınlarda HR HPV prevalansını %27, Abike ve arkadaşları %26, Aydoğan ve arkadaşları %34.5, Tezcan ve arkadaşları %42.9, Beyazıt ve arkadaşları %62, Baskaran ve arkadaşları %65.7, Ergünay ve arkadaşları %100 oranında bulmuşlardır [8][9][10]12,13,15,[16][17][18][19][20][21] . HSIL sitolojili kadınlardaki %66.7'lik HR HPV prevalansı Aydoğan ve arkadaşlarının %7'lik Dursun ve arkadaşlarının %20'lik oranından yüksek, Tezcan ve arkadaşlarının %75'lik oranına yakın, Assoumou ve arkadaşlarının %100, Ergünay ve arkadaşlarının %100, Tabora ve arkadaşlarının %97, Corenti ve arkadaşlarının %95, Kim ve arkadaşlarının %96.7, Abike ve arkadaşlarının %91, Jiang ve arkadaşlarının %90, Baskaran ve arkadaşlarının %84.6, Beyazıt ve arkadaşlarının %83 ve Simaviciene ve arkadaşlarının %80.3 oranlarından düşük tespit edilmiştir [8][9][10][11][12][14][15][16][17][18][19][20][21] .…”

HPV prevalence and type‐distribution in cervical cancer and premalignant lesions of the cervix: A population‐based study from Northern Ireland

“…The prevalence (including HPV negative cases in the denominator) of HPV-16 was 27.6%, 39.8%, and 58.2% and HPV-18 prevalence 9.0%, 10.0%, and 7.4% in CIN I-III cases respectively [Guan et al, 2012], again higher than the estimates reported in the present study, variation which may have arisen from methodological differences in HPV detection techniques. The majority of SCCs (92.2%) in the present study were HPV positive, this is in accordance with the proportions of invasive cervical cancers (the majority of which were SCCs) testing HPV positive in other countries of the UK [Mesher et al, 2015], Sweden, Including high-risk HPV genotypes other than HPV 16/18 that is: 33,35,39,45,51,52,56,58,59,66,and 68. Spain Italy, and Eastern Europe [Du et al, 2011;Alemany et al, 2012;Giorgi Rossi et al, 2012;Guan et al, 2012;Piana et al, 2013;Pista et al, 2013;Tjalma et al, 2013;Skamperle et al, 2013;Kjaer et al, 2014;Simanaviciene et al, 2015].…”

Different DNA methylation pattern of HPV16, HPV18 and HPV51 genomes in asymptomatic HPV infection as compared to cervical neoplasia

“…Cervical samples were collected from the cervix using cervical brush during our previous study on the prevalence of oncogenic HPV types among Lithuanian women with cervical pathology (Simanaviciene et al, 2015). The study was approved by Vilnius Regional Committee of Biomedical Research (Lithuania, permission no.…”

“…CRL-1550) were purified using "GeneJET ™ Genomic DNA Purification Kit" according to manufacturer's instruction. Detailed description of collected cervical specimens and the procedure of DNA extraction from cervical specimens have been presented previously (Simanaviciene et al, 2015).…”