Studies on the production of actinomycin-D by<i>Streptomyces griseoruber</i>- a novel source

Praveen, Vandana; Tripathi, C. K. M.

doi:10.1111/j.1472-765x.2009.02689.x

Cited by 31 publications

(20 citation statements)

References 28 publications

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“…Streptomyces griseoruber produces antitumor and antibacterial antibiotics (Helaly et al 2009;Praveen and Tripathi 2009). To our knowledge, this is the first report of S. griseoruber as an antagonist for clubroot.…”

Section: Discussionmentioning

confidence: 75%

A strain of Streptomyces griseoruber isolated from rhizospheric soil of Chinese cabbage as antagonist to Plasmodiophora brassicae

et al. 2011

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To find potential biocontrol agents for Plasmodiophora brassicae, a total of 63 actinobacteria were isolated from the rhizospheric soil of Chinese cabbage that were collected from Sichuan province in China. Isolates were screened by measuring the resting spores' germination inhibition. The suppressive effects of six antagonistic strains against clubroot on Chinese cabbage in the glasshouse and the field were assessed, respectively. Strain A316 was the most promising antagonist against clubroot, which showed the highest control values of 73.69% in the glasshouse experiment and 65.91% in the field trial. Based on the 16S rDNA sequence analysis, strain A316 was most closely related to Streptomyces griseoruber NBRC 12873 (99.9% similarity). Together with morphological, cultural and physio-biochemical characteristics, strain A316 was identified as Streptomyces griseoruber. The results indicate that the isolate A316 might have the potential possibility to be used as an antagonist against P. brassicae. This is the first report of S. griseoruber for controlling clubroot.

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Section: Discussionmentioning

confidence: 75%

A strain of Streptomyces griseoruber isolated from rhizospheric soil of Chinese cabbage as antagonist to Plasmodiophora brassicae

et al. 2011

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“…Isolation of genomic DNA, polymerase chain reaction (PCR) amplification, and the sequencing of PCR-amplified product was carried out according to the methods described earlier [13]. Closely related homolog were identified by comparing partial 16S rRNA sequence with the sequences deposited in database at National Center for Biotechnology Information (NCBI) web server (http://www.ncbi.nlm.nih.gov), using the standard nucleotide-nucleotide BLAST program [14].…”

Section: Taxonomic Studies Of the Production Strainmentioning

confidence: 99%

Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Praveen

Srivastava

Tripathi

2011

Appl Biochem Biotechnol

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An extracellular cholesterol oxidase (cho) enzyme was isolated from the Streptomyces parvus, a new source and purified 18-fold by ion exchange and gel filtration chromatography. Specific activity of the purified enzyme was found to be 20 U/mg with a 55 kDa molecular mass. The enzyme was stable at pH 7.2 and 50 °C. The enzyme activity was inhibited in the presence of Pb(2+), Ag(2+), Hg(2+), and Zn(2+) and enhanced in the presence of Mn(2+). The enzyme activity was inhibited by the thiol-reducing reagents (DTT, β-mercaptoethanol), suggesting that disulfide linkage is essential for the enzyme activity. The enzyme activity was found to be maximum in the presence of Triton X-100 and X-114 detergents whereas sodium dodecyl sulfate fully inactivated the enzyme. The enzyme showed moderate stability towards all organic solvents except acetone, benzene, chloroform and the activity increased in the presence of isopropanol and ethanol. The K(m) value for the oxidation of cholesterol by this enzyme was 0.02 mM.

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“…Actinomycins, such as Act‐D are chromopeptide lactone antibiotics of which more than 30 native and many synthetic variants are known. To date, 27 Streptomyces species and one Micromonospora have been reported to produce various forms of actinomycins . This is the first report of a representative of S .…”

Section: Discussionmentioning

confidence: 88%

Antifungal properties of an actinomycin D‐producing strain, Streptomyces sp. IA1, isolated from a Saharan soil

Toumatia

Yekkour

Goudjal

et al. 2014

J. Basic Microbiol.

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An actinomycete strain named IA1, which produced an antimicrobial compound, was isolated from a Saharan soil in In Amenas, Algeria. The study of the 16S rDNA sequence of this strain permitted to relate it to Streptomyces mutabilis NBRC 12800(T) (99.93% of similarity). Strain IA1 exhibited strong activity against a wide range of plant pathogenic fungi. One bioactive compound produced in large amounts (46.7 mg L(-1) day(-1) ), named YA, was isolated and purified by TLC and reverse phase HPLC. The structure elucidation of the pure substance, using combined data from UV visible, NMR spectra, and mass spectrometry, permitted to identify it as actinomycin D, and was thus found for the first time in S. mutabilis related species. The biocontrol abilities of the strain IA1 and compound YA were evaluated through two diseases, i.e., chocolate spot of field bean and Fusarium wilt of flax. The occurrence of the two fungal diseases was effectively reduced. The reduction of chocolate spot disease symptoms reached 80 and 91.7% with IA1 and YA seedlings pretreatments, respectively. Soil pretreatment with IA1 or YA also allowed to reduce Fusarium wilt disease impact by almost 60%.

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Studies on the production of actinomycin-D byStreptomyces griseoruber- a novel source

Cited by 31 publications

References 28 publications

A strain of Streptomyces griseoruber isolated from rhizospheric soil of Chinese cabbage as antagonist to Plasmodiophora brassicae

A strain of Streptomyces griseoruber isolated from rhizospheric soil of Chinese cabbage as antagonist to Plasmodiophora brassicae

Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Antifungal properties of an actinomycin D‐producing strain, Streptomyces sp. IA1, isolated from a Saharan soil

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