Studies on the Propagation in Vitro of Poliomyelitis Viruses

Scherer, William F.; Syverton, Jerome T.; Gey, George O.

doi:10.1084/jem.97.5.695

Cited by 756 publications

(253 citation statements)

References 20 publications

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“…Jurkat cells [15] and the human Burkitt lymphomas Raji [16], Rael [17], BL36 [18], BL41 [19] and Mutu-BL III (kindly provided by Dr V. Levitsky, MTC, Karolinska Institute, Sweden) were cultured as the A20 cells but in the absence of 2-mercaptoethanol. The human retroviral packaging cell line phoenix-ampho (available at http://www.uib.no/mbi/nolan/NLphoenix.html) and HeLa cells [20] were grown in Dulbecco's modified Eagle's medium supplemented with 10% FCS, 2 mm l-glutamine, 50 U/ ml penicillin, 50 mg/ml streptomycin and 1 mm sodium pyruvate. Around 15 ml of blood in EDTA was diluted 1 : 1 with RPMI and carefully added on top of 15 ml of Ficoll-Paque TM research grade (Amersham Pharmacia Biotech, Amersham Pharmacia Biotech, Uppsala, Sweden).…”

Section: Methodsmentioning

confidence: 99%

Characterization of the Human FLICE‐Inhibitory Protein Locus and Comparison of the Anti‐Apoptotic Activity of Four Different FLIP Isoforms

et al. 2001

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Death receptor-mediated apoptosis is involved in the regulation of immune responses and in the maintenance of immunological tolerance. FLICE-inhibitory proteins (FLIPs) are important modulators of death receptormediated apoptosis. To date, the FLIP family encompasses multiple members, of which some are reported to be antiapoptotic and others pro-apoptotic. This led us to investigate the activity of several FLIP proteins in vitro. Concomitant with the cloning of various FLIP isoforms, a new and unexpected member of the FLIP family, denoted FLIP R , was isolated from the human Burkitt lymphoma B-cell line Raji. During the characterization of FLIP R , the genomic sequence of human FLIP was found in the NCBI GenBank. This enabled us to present the complete exon-intron constellation of the human FLIP gene and the generation of all known human FLIP isoforms by alternative splicing. We show that the human FLIP gene with a size of approximately 48 kb, consists of at least 14 exons and can give rise to 11 distinct isoforms by alternative splicing. When studying the activity of some of these isoforms, including FLIP R , they all efficiently inhibited Fas-mediated apoptosis in A20 B lymphoma cells by impeding caspase-8, -3 and -7 activity as well as poly(ADP-ribose) polymerase (PARP) cleavage.

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Section: Methodsmentioning

confidence: 99%

Characterization of the Human FLICE‐Inhibitory Protein Locus and Comparison of the Anti‐Apoptotic Activity of Four Different FLIP Isoforms

et al. 2001

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“…The following human neuroblastoma cell lines were used: SK-N-SH (Biedler et al, 1973), SK-N-BE(2c) (Biedler et al, 1978), NB-100 (Schlesinger et al, 1976), IMR-32 (Tumilowicz et al, 1970), NBI-G (Carachi et al, 1987) (Scherer et al, 1953) and ovarian carcinoma A2780, a variant of the line NIH:OVCAR-3 (Hamilton et al, 1983). AU ceUs were cultured in Eagle's minimal essential medium supplemented with 10% fetal calf serum, 2 mM glutamine, penicillin-streptomycin (100 IU ml-') and amphotericin B (2.5 ;Lg ml-').…”

Section: Cell Culturementioning

confidence: 99%

Prediction of accumulation of 131I-labelled meta-iodobenzylguanidine in neuroblastoma cell lines by means of reverse transcription and polymerase chain reaction

Mairs

Livingstone²,

Gaze³

et al. 1994

Br J Cancer

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S_inaary Radiolabelled meta-iodobenzylguanidine (mIBG) currently provides one of the most promising options for targeted radiotherapy of neuroblastoma. No means currently exists for prediction of mIBG uptake in tumour cells of individual patients other than semiquantitative inferences from diagnostic scanning which depend on the continued existence of a macroscopic tumour mass. A biological rapid assay which could be applied at initial biopsy would be invaluable in selecting patients for therapeutic strategies which incorporate radiolabeled mIBG. We have assesed the expression of the noradrenahne transporter gene in six human neuroblastoma cell lines and in three non-neural crest-derived cell lines using reverse transcription followed by the polymerase chain reaction. Transcription of this gene was observed in five out of six neuroblastoma cell lines but in none of the control cells. A highly significant correlation was established (P<0.O1) between gene expression and active cellular accumulation of mIBG. It is suggested that semiquantitative evaluation of noradrenaline transporter gene transcripts may be predictive of mIBG uptake by tumours in vivo.

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“…Tissue Culture.--Epithelial cells of the HeLa strain (6) were employed in all experiments and were propagated in either Eagle's basal medium (7) containing 10 per cent human serum, or as previously described (8), in a growth fluid composed of 40 per cent human serum and Hanks' balanced salt solution.…”

Section: Methodsmentioning

confidence: 99%

Intracellular Localization of Type 4 Adenovirus

Denny¹,

Ginsberg²

1959

The Journal of Experimental Medicine

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Electron microscopic studies by several investigators (1-3) have shown virus-like particles in the nuclei of HeLa cells infected with adenoviruses. Morgan and his associates (3) showed disruption of the nuclear membrane in a few cells with release of virus-like particles into the cytoplasm, but did not note viral development in the cytoplasm. A tremendous number of particles have been observed by electron microscopic examination of thin sections of infected HeLa cells, a finding which seems to be out of proportion to the relatively low yield of infectious virus (2) thus suggesting that most of the particles seen are not infectious, or that the large number of intranuclear particles remain aggregated and therefore act as relatively few infectious units. Characteristic nuclear changes in HeLa cells infected with adenoviruses have also been described using light microscopy (4); these alterations have been correlated with formation of specific viral antigen and infectious virus (5). Cytoplasmic changes were also described, but were much less marked and not characteristic (4). Because microscopic observations cannot disclose the site of development of infectious virus, and because it seemed possible that a large proportion of the particles visualized were not infectious, studies were undertaken to determine the intracellular localization of infectious adenovirus. In the experiments described in this report, nuclear and cytoplasmic fractions of HeLa cells infected with adenoviruses were obtained by mechanical disruption and differential centrifugation, and infectious virus in these fractions was measured.The results obtained indicated that the majority of infectious type 4 adenovirus was isolated from the cytoplasm of the HeLa cell; experiments done with type 1 adenovirus, although not conclusive, yielded somewhat different results. The techniques used in these experiments have many limitations and *

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Studies on the Propagation in Vitro of Poliomyelitis Viruses

Cited by 756 publications

References 20 publications

Characterization of the Human FLICE‐Inhibitory Protein Locus and Comparison of the Anti‐Apoptotic Activity of Four Different FLIP Isoforms

Characterization of the Human FLICE‐Inhibitory Protein Locus and Comparison of the Anti‐Apoptotic Activity of Four Different FLIP Isoforms

Prediction of accumulation of 131I-labelled meta-iodobenzylguanidine in neuroblastoma cell lines by means of reverse transcription and polymerase chain reaction

Intracellular Localization of Type 4 Adenovirus

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