Studies on the receptors in Ciona intestinalis. IV. The ocellus in the adult

Dilly, P. N.; Wolken, Jerome J.

doi:10.1016/0047-7206(72)90003-9

Cited by 18 publications

(21 citation statements)

References 16 publications

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“…In transgenic animals of the E15 enhancer trap line, which express GFP in the nervous system (Awazu et al, 2007), GFP staining was detected in the basal portion of the receptor cells (Figs. 1F, G), suggesting that this region could function as a neural component (also see Dilly and Wolken, 1973). Bands of yellow ( Ciona from Woods Hole and Roscoff) or orange (E15 Ciona strain from Japan) pigment cells lie between the pigment organs along the distal margins of the siphons (Figs.…”

Section: Resultsmentioning

confidence: 86%

“…3B, E), and DAPI (a marker of polarized basal nuclei) (Fig. 3C) (Dilly and Wolken, 1973), and regeneration of the putative neural component of the OPO was determined by GFP fluorescence in E15 transgenic animals (Figs. 3D, E).…”

Section: Resultsmentioning

confidence: 99%

“…These pigment organs are sometimes referred to as ocelli or photoreceptor organs, although their ability to detect light and transmit sensory information through neural connections has not been established (Hecht, 1918; Millar, 1953; Dilly and Wolken, 1973). Here we investigate the regeneration of ocellus-like pigment organs (oral pigment organs or OPO) in the oral siphon of C. intestinalis .…”

Section: Introductionmentioning

confidence: 99%

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Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis

Auger

Sasakura²,

Joly³

et al. 2010

Developmental Biology

141

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Ascidians have powerful capacities for regeneration but the underlying mechanisms are poorly understood. Here we examine oral siphon regeneration in the solitary ascidian Ciona intestinalis. Following amputation, the oral siphon rapidly reforms oral pigment organs (OPO) at its distal margin prior to slower regeneration of proximal siphon parts. The early stages of oral siphon reformation include cell proliferation and re-growth of the siphon nerves, although the neural complex (adult brain and associated organs) is not required for regeneration. Young animals reform OPO more rapidly after amputation than old animals indicating that regeneration is age dependent. UV irradiation, microcautery, and cultured siphon explant experiments indicate that OPOs are replaced as independent units based on local differentiation of progenitor cells within the siphon, rather than by cell migration from a distant source in the body. The typical pattern of eight OPOs and siphon lobes is restored with fidelity after distal amputation of the oral siphon, but as many as 16 OPOs and lobes can be reformed following proximal amputation near the siphon base. Thus, the pattern of OPO regeneration is determined by cues positioned along the proximal distal axis of the oral siphon. A model is presented in which columns of siphon tissue along the proximal–distal axis below pre-existing OPO are responsible for reproducing the normal OPO pattern during regeneration. This study reveals previously unknown principles of oral siphon and OPO regeneration that will be important for developing Ciona as a regeneration model in urochordates, which may be the closest living relatives of vertebrates.

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis

Auger

Sasakura²,

Joly³

et al. 2010

Developmental Biology

141

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“…Detailed ultrastructural descriptions of the dorsal melanocyte, the ciliary photoreceptor cells, and the lens cells of Ciona intestinalis tadpoles have been reported by Dilly (1964), Eakin and Kuda (1971), and Eakin (1973). (See also Barnes [1971Barnes [ , 1974 for similar de- scriptions of the tadpole of the tunicate Amaroucium constellatum, and see Dilly and Wolken [1973] for a description of the ocellus of adult Ciona. Eakin [1973] and Salvini-Plawen and Mayr [1977] provide comprehensive general discussions of the evolution of visual organs.…”

Section: A Prosencephalonmentioning

confidence: 98%

COMPARATIVE ANATOMY OF THE TUNICATE TADPOLE,CIONA INTESTINALIS

Katz¹

1983

The Biological Bulletin

196

110

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“…Among the organs capable of regeneration are the neural complex (including the brain, or cerebral ganglion, of the central nervous system) (Hirschler, 1914; Bollner, et al, 1992; Dahlberg et al, 2009) and the oral and atrial siphons (Fox, 1924; Von Haffner, 1933; Sutton, 1953; Whittaker, 1975; Auger et al, 2010). The siphons contain pigmented organs on their distal margins, which have been proposed to function as sensory receptors (Dilly and Wolken, 1973). There are usually eight oral pigment organs (OPO) located in notches between muscular lobes in the oral siphon (OS).…”

Section: 1 Introductionmentioning

confidence: 99%

Siphon regeneration capacity is compromised during aging in the ascidian Ciona intestinalis

Jeffery

2012

Mechanisms of Ageing and Development

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The ascidian Ciona intestinalis has a short life span and powerful regeneration capacities. The regeneration of the oral siphon (OS) involves wound healing, blastema formation, cell proliferation, and replacement of eight oral pigment organs (OPO), the latter via differentiation and migration of stem/precursor cells from localized siphon niches in the siphon. The restoration of OPO pattern during OS regeneration occurs with a high degree of accuracy through three successive cycles of amputation. It is shown here that oral siphons of the largest and oldest members of a wild Ciona population do not completely regenerate their siphons after amputation. The loss of regeneration capacity was accompanied by reduced cell proliferation. In contrast to arrested OS outgrowth, the stem/precursor cells responsible for OPO replacement “over-differentiate” after OS amputation in the oldest animals, the typical number of OPO is increased from eight to twelve-sixteen, and malformed OPO are produced. Also in contrast to younger animals, the oldest animals of the population show arrested OPO development after two consecutive cycles of amputation and regeneration. We conclude that there is a size and age threshold in Ciona after which the regenerative capacity of the OS is compromised due to effects of aging on cell proliferation.

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Studies on the receptors in Ciona intestinalis. IV. The ocellus in the adult

Cited by 18 publications

References 16 publications

Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis

Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis

COMPARATIVE ANATOMY OF THE TUNICATE TADPOLE,CIONA INTESTINALIS

Siphon regeneration capacity is compromised during aging in the ascidian Ciona intestinalis

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