Studies on the regeneration of the skin barrier and the changes in 32P incorporation into the epidermis after stripping

Komatsu, Hajime; Suzuki, Masami

doi:10.1111/j.1365-2133.1982.tb04558.x

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…We believe, however, it is caused by the process of regeneration of epidermis with time, which varies between species. Morphologic observations in rabbits after tape stripping showed that a parakeratotic layers was present at 3 d, when the permeability was markedly reduced, and distinct hyperplasia was observed at 5-7 d (Komatsu and Suzuki, 1982). Hairless mice, however, showed that the increased TEWL was reversed and barrier repair was complete within 24-36 h (Jiang et al, 1998).…”

Section: Discussionmentioning

confidence: 98%

“…It leads to hyperproliferation without severe inflammation (Rijzewijk et al, 1988). Morphologic studies that have been performed on rabbit (Komatsu and Suzuki, 1982) and guinea pig (Braun-Falco and Burg, 1971) showed the appearance of parakeratotic cells and the occurrence of hyperplasia after tape stripping in the air-exposed model. In this study, we demonstrated that the transitional cell layers appeared with occlusion after tape stripping but not in the air-exposed model.…”

Section: Discussionmentioning

confidence: 99%

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The Changes of Epidermal Calcium Gradient and Transitional Cells after Prolonged Occlusion Following Tape Stripping in the Murine Epidermis

Ahn

Hwang

Jiang

et al. 1999

Journal of Investigative Dermatology

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Disruption of the epidermal permeability barrier causes an immediate loss of the calcium gradient, and barrier recovery is parallel with the restoration of the calcium gradient in the epidermis. Artificial restoration of the barrier function by occlusion with a water vapor-impermeable membrane abrogate the expected increase in lipid synthesis and retard the barrier recovery, as well as block the normalization of the epidermal calcium gradient. To clarify the long-term effects of occlusion after acute barrier perturbation, we studied the calcium distribution and epidermal keratinocytes response after occlusion with a water vapor-impermeable membrane immediately following tape stripping in the murine epidermis. Acute barrier disruption caused an immediate depletion of most calcium ions in the upper epidermis, obliterating the normal calcium gradient. When the skin barrier function was artificially corrected by occlusion, the return of calcium ions to the epidermis was blocked. After 2 h of air exposure or occlusion, the density of epidermal calcium precipitates remained negligible. The transitional cell layers appeared with occlusion, but not or negligibly with air exposure. By 6 h though, calcium precipitates could be seen, the density of the calcium precipitates with occlusion was more sparse than with air exposure. With the air exposure, the thickness of the stratum corneum had normalized and the calcium gradient nearly recovered to normal after 24 h. The longer the occlusion period, the greater was the increase of transitional cells. By 60 h of occlusion, the thickness of the stratum corneum had increased and the transitional cell layers had disappeared, in parallel with the calcium gradient which was almost normalized. These results show that prolonged occlusion of tape-stripped epidermis induced transitional cells and delayed the restoration of the epidermal calcium gradient, the stratum corneum was then restored, transitional cells having disappeared, in parallel with normalization of the epidermal calcium gradient.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

The Changes of Epidermal Calcium Gradient and Transitional Cells after Prolonged Occlusion Following Tape Stripping in the Murine Epidermis

Ahn

Hwang

Jiang

et al. 1999

Journal of Investigative Dermatology

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Contact Dermatitis: Irritant

Wilkinson¹,

Beck²

2004

Rook's Textbook of Dermatology

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Functional and structural changes of the epidermal barrier induced by various types of insults in hairless mice

Ahn

Jiang

Hwang

et al. 2001

Archives of Dermatological Research

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Acute barrier disruption, regardless of the method of induction, depletes the stratum corneum intercellular lipids and this stimulates a series of lipid/ DNA synthesis activities which lead to barrier recovery. After barrier disruption by tape stripping, occlusion with a water vapor-impermeable membrane inhibits barrier repair. In this study, we investigated the changes in the murine epidermis after barrier perturbation by tape stripping and three different types of surfactants (Emalex NP-12, ENP-12; sodium dodecyl sulfate, SDS; benzalkonium chloride, BKC). To examine the effect of an artificial barrier, we covered the animals with a water vapor-impermeable membrane for 3 days following barrier disruption and then exposed them to the air for 2 days. The histological findings after occlusion or air exposure were similar. However, after air exposure for 2 days, the thickness of the epidermis including the stratum corneum and the stratum granulosum layers decreased to about half that of the epidermis after occlusion. Ultrastructural examination revealed obvious distortion of the lamellar bilayers within the stratum corneum interstices immediately after barrier disruption. After 3 days of occlusion, extensive disorganization was evident in the intercellular domain of the stratum corneum, whereas 2 days after removal of the occlusion, the normal basic unit structure of the lamellar bilayers had partially reappeared. Our findings provide evidence that the kinetic pattern of barrier repair and the morphological changes are similar after occlusion following barrier disruption regardless of the mechanism of disruption.

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Studies on the regeneration of the skin barrier and the changes in 32P incorporation into the epidermis after stripping

Cited by 5 publications

References 26 publications

The Changes of Epidermal Calcium Gradient and Transitional Cells after Prolonged Occlusion Following Tape Stripping in the Murine Epidermis

The Changes of Epidermal Calcium Gradient and Transitional Cells after Prolonged Occlusion Following Tape Stripping in the Murine Epidermis

Contact Dermatitis: Irritant

Functional and structural changes of the epidermal barrier induced by various types of insults in hairless mice

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