Studies on the relationship between concanavalin A and SV40-transformed human fibroblasts

Abstract

Summary.-The extent of binding of 1251-Con A to the surface of SV40-transformed human fibroblasts and the degree of agglutination of the cells by the native lectin have been measured. In addition, trypsinized and succinylated Con A have been used to study the effects of the lectin upon certain cell growth parameters. Trypsinized Con A was found to alter the growth rate, the saturation density and the contact inhibition of the transformed cells, an effect not neutralized by oz-methyl-D-mannoside.

Sulphated acid mucopolysaccharides in SV40-transformed human cells from normal and mucopolysaccharidosis patients

“…The monovalent lectin binds to the surface of both normal and transformed cells, but has been found to alter the growth rate of only the transformed cells (Webb, 1976 …”