Studies on the role of goat heart galectin-1 as an erythrocyte membrane perturbing agent

Abstract

Galectins are β-galactoside binding lectins with a potential hemolytic role on erythrocyte membrane integrity and permeability. In the present study, goat heart galectin-1 (GHG-1) was purified and investigated for its hemolytic actions on erythrocyte membrane. When exposed to various saccharides, lactose and sucrose provided maximum protection against hemolysis, while glucose and galactose provided lesser protection against hemolysis. GHG-1 agglutinated erythrocytes were found to be significantly hemolyzed in comparison with unagglutinated erythrocytes. A concentration dependent rise in the hemolysis of trypsinized rabbit erythrocytes was observed in the presence of GHG-1. Similarly, a temperature dependent gradual increase in percent hemolysis was observed in GHG-1 agglutinated erythrocytes as compared to negligible hemolysis in unagglutinated cells. The hemolysis of GHG-1 treated erythrocytes showed a sharp rise with the increasing pH up to 7.5 which became constant till pH 9.5. The extent of erythrocyte hemolysis increased with the increase in the incubation period, with maximum hemolysis after 5 h of incubation. The results of this study establish the ability of galectins as a potential hemolytic agent of erythrocyte membrane, which in turn opens an interesting avenue in the field of proteomics and glycobiology.

Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

“…This result is similar to what was obtained by Ashraf et al. , [74] for galectin isolated from the goat heart muscle.

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The apoptotic effect of resveratrol in ovarian cancer cells is associated with downregulation of galectin‐3 and stimulating miR‐424‐3p transcription

“…Currently, it is well accepted that GALs can regulate numerous cell function such as inflammation and survival by regulating various cell signaling pathways (Ashraf et al, ; Ashraf, Perveen, Tabrez, et al, ; Ashraf, Perveen, Zaidi, et al, ). GAL‐3 was shown to stimulate cell survival through activation of Akt and NF‐κB signaling and plays a crucial role in promoting tumorigenesis and drug resistance of various tumors including OC (Mirandola, Yu, et al, ).…”

“…On the other hand, available literature reports relevant roles of galectins (GALs) in modulation of acute inflammatory response and protection against several disorders (Ashraf, Mahmoud, & Tabrez, ; Ashraf, Perveen, Tabrez, et al, ; Ashraf, Perveen, Zaidi, et al, ). Galectin‐3 (GAL‐3) (coded by LGALS3 gene) is an S‐type lectin that binds β‐galactose‐containing glycoconjugates and is highly expressed on the surface, nuclei, and cytoplasm of the cells and is abundantly found in the circulation (Mirandola, Yu, et al, ).…”