Studies on the Structure of T Lymphocyte Receptors using Xenogeneic Anti‐idiotype Antibodies

Rubin, Bent; Hertel-Wulff, B

doi:10.1111/j.1365-3083.1978.tb00488.x

Cited by 10 publications

(12 citation statements)

References 9 publications

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“…Pregnant rabbits were purchased from Statens Seruminstitut. Newborn rabbits were made neonatally tolerant to mouse y-globulin (MyG) as described previously (21). Adult outbred rabbits produced test antisera as described (2 l, 22).…”

Section: Methodsmentioning

confidence: 99%

“…M'IA3 was prepared from NMS or from anti-H-2 antisera as described previously (21). IgG preparations were obtained by DEAE-Sephacel (Pharmacia Fine Chemicals, Inc., Uppsala, Sweden) fractionation.…”

Section: Methodsmentioning

confidence: 99%

“…Inbred mice were maintained in our breeding colony at the Immunobiology Laboratory, Statens Seruminstitut Copenhagen, Denmark (21,22) Center, Uppsala University, Uppsala, Sweden. Strain B65a mice were produced by backcrossing (B6 X CBA)F1 mice to B6 mice.…”

Section: Methodsmentioning

confidence: 99%

“…All preparations were adjusted to 10 mg/ml. Protein determinations, purity evaluations and the micro-Ouchterlony assay were described previously (21).…”

Section: Methodsmentioning

confidence: 99%

“…We have initiated a study on these problems using as probes anti-Id antibodies raised against mouse anti-H-2 antibodies. Because we have shown previously (a) that such anti-Id antibodies can be produced in relatively large amounts, and (b) that they react against both Tcr and B-cell receptor (Bcr) molecules of the same specificity (21), these reagents can be used for isolation and purification of Id-positive (Id +) Tcr(s). Studies on the structure of such purified Tcr(s) would demonstrate directly which of the aforementioned determinants were present.…”

mentioning

confidence: 99%

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Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Rubin¹,

Hertel-Wulff²,

Kimura³

1979

The Journal of Experimental Medicine

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The present study describes the qualitative reactions of a xenogeneic anti-idiotype (Id) antiserum produced in a mouse-gamma-globulin-tolerant rabbit (5,936) against B6 anti-CBA IgG antibodies. The results showed that such an anti-Id antiserum reacts specifically against anti-H-2k antibodies and against H-2k alloantigen-activated T cells from the following pairs of congenic mice: B10 (H-2b) and B10.D2 (H-2d); and A.BY (H-2b) and A.SW (H-2s), but not against C3H.SW (H-2b) and C3H.OH (H-2o); and BALB/b (H-2b) and BALB/c (H-2d). CB 20 (BALB/c mice with the Ig-1b allotype) anti-CBA T blasts also express idiotypic determinants that react with rabbit 5,936 antiserum. Thus, positive reactions are obtained between rabbit 5,936 anti-Id antiserum and anti-H-2k IgG preparations and T blasts from mice carrying the Ig-1b or Ig-1e allotype, but not from mice carrying the Ig-1a allotype. These reactions are qualitatively independent of the H-2 genotype of the Id-producing mice. Such a finding strongly suggests that the Id-bearing receptor molecules on mouse T cells are coded for by genes that are associated with the Ig heavy-chain-linkage group and not to the mouse histocompatibility complex. Furthermore, the anti-Id antibodies studied react preferentially against anti-H-2k antibodies or T cells with specificity toward the IAk-region-associated serological specificities. Thus, genes associated with the Ig heavy-chain-linkage group seem to be structural genes for at least T-cell receptors with specificity for IA-region-coded membrane antigens.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…All preparations were adjusted to 10 mg/ml. Protein determinations, purity evaluations and the micro-Ouchterlony assay were described previously (21).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Rubin¹,

Hertel-Wulff²,

Kimura³

1979

The Journal of Experimental Medicine

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Recognition of antigens by T lymphocytes

Binz

Wigzell

1978

Mol Cell Biochem

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The present review briefly summarizes our knowledge of antigen-specific B and T lymphocyte receptors. Antigen-specific receptors on mammalian B lymphocytes are mainly monomeric IgM and IgD consisting of conventional immunoglobulin heavy and light chains. The nature of the T lymphocyte receptor which can specifically recognize antigens is not yet fully defined. However, it seems that conventional light chains do not participate in the build up of this receptor, and that the receptor is made up of heavy chains of a new immunoglobulin class which has to be further characterized and which we call Tau-chain. The variable region of the T lymphocyte receptors share idiotypic determinants with the corresponding B lymphocyte receptors. The possible linkage between the T cell idiotypes present on the Tau-chains and molecules which are under the control of genes of the Major Histocompatibility Complex of the species are discussed. In the last part of the review two methods for the induction of specific transplantation tolerance in adult animals are described. These methods are based on the concept that T lymphocytes reactive against alloantigens bear idiotypic determinants against which a specific auto-immune response can be initiated.

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Idiotypic regulation of mouse anti-H-2 antibody responses

Nordfang

Rubin

1981

Immunogenetics

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Mouse-immunoglobulin (MIg) tolerant rabbits immunized with mouse H-2 antibodies produced anti-idiotype antisera, which were reactive towards specific B- and T-cell receptors. One such rabbit antiserum (from rabbit 5936) defines a family of idiotypes (Id) designated 5936-idiotypes (Rubin et al. 1979). The present experiments were performed in order to establish (1) the nature of 5936-Id+ serum molecules, (2) the specificity of 5936-Id+ serum molecules, (3) the association of the 5936-Id genes to allotype and/or H-2 genes and (4) the immunological role of 5936-Id+ serum molecules. A sensitive radioimmunoassay employing 125I-labelled-F(ab)2 fragments of B6 anti-B10.BR MIg pool, 5936 antiserum, and a sheep anti-rabbit immunoglobulin antiserum, was used.--The results suggested that 5936-Id+ serum molecules were exclusively MIg, and that they were mainly of the IgG1 class. Such molecules were induced in B6 mice (H-2b/Ig-1b) upon immunization with H-2k but not with H-2q alloantigen or conventional antigens. The 5936-Id were found to be associated with Ig-1b allotypes and the H-2b complex may contain immune response (Ir) genes which, in comparison with Ir genes in H-2d and H-2s, favor the expression of 5936-Id.--Adsorption of 5936-Id+ B6 anti-CBA MIg preparations on CBA (IAk) spleen cells demonstrated that CBA antibodies were 5936-Id-. It is discussed whether 5936-Id+, IgG1 molecules in B6 anti-CBA sera are anti-(anti-CBA) antibodies or nonspecific antibodies, the production of which is augmented by immunization with IAk alloantigen.

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Studies on the Structure of T Lymphocyte Receptors using Xenogeneic Anti‐idiotype Antibodies

Cited by 10 publications

References 9 publications

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Recognition of antigens by T lymphocytes

Idiotypic regulation of mouse anti-H-2 antibody responses

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