1969
DOI: 10.1139/m69-259
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Studies on the taxonomy of the Myxohacterales. I. Record of Canadian isolates and survey of methods

Abstract: One hundred and sixty-seven pure cultures have been isolated from among 338 myxobacteria obtained from Essex county (Ontario) soil samples by various methods. The fruiting body characteristics used in the characterization of the Myxobacterales have been found to be variable and unstable. However, the order may be considered to consist of two broad groups based on vegetative cell morphology and the formation of microcysts. A proposal is made to divide the genus Chondromyces into Stigmatella and Chondromyces and… Show more

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Cited by 80 publications
(46 citation statements)
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“…Gliding Myxococcus cells deposit a slime trail, and the dye Congo red is adsorbed by Myxococcus colonies (29,30). Arnold and Shimkets (2) have correlated Congo red binding with a loose network of thick fibers that surrounds cells and adheres to agar.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Gliding Myxococcus cells deposit a slime trail, and the dye Congo red is adsorbed by Myxococcus colonies (29,30). Arnold and Shimkets (2) have correlated Congo red binding with a loose network of thick fibers that surrounds cells and adheres to agar.…”
Section: Resultsmentioning
confidence: 99%
“…Colonies were stained for slime components by flooding the colony with 400 ,ul of an aqueous solution of 0.02% Congo red and 100 mM sodium phosphate (pH 7.7) (2,29,30 (wild type), and DK1250 (A-S-) were grown in CTT broth to a cell density of 4 x 10' to 5 x 108 cell per ml (100 Klett units [20]) and adjusted to 5 and 25 Klett units by dilution in a 1:1 (by volume) CTT deionized water solution. Activated charcoal was added to each sample to a final concentration of 50 ,ug/ml.…”
mentioning
confidence: 99%
“…Flexirubin pigments were detected by suspending cells in 20 % (w\v) KOH, with a colour change from yellow or orange to brown-red indicative of a positive result (Fautz & Reichenbach, 1980). The detection of extracellular glucans by the Congo red absorption test was performed by flooding plates with 0n01 % (w\v) Congo red (McCurdy, 1969). The procedures of Hildebrand (1971) were followed to test for the degradation of pectin.…”
Section: Methodsmentioning
confidence: 99%
“…Reaction of the vegetative cells to Gram-staining was determined according to the established protocol (Gerhardt et al, 1981), while Congo red staining was performed according to the method described by McCurdy (1969). The catalase test was performed with a drop of 3.0 % H 2 O 2 on actively growing vegetative cells, while an oxidase test was carried out using a commercial test strip (Sigma-Aldrich).…”
mentioning
confidence: 99%