Studies on the Tissue Concentrations of TRF in the Normal Dog

Hypothalamic Neurohumoral Control of Pituitary Function

“…The time relations of these thyroid responses are open to question, the extracts are undoubtedly contaminated with many hypothalamic substances including vasopressin, and no hypophysectomized control animals have been used-concurrently in these studies to rule out the possibility that the extracts are acting on the thyroid and not causing the pituitary release of TSH. Using column chromatography, this group has isolated a fraction 'T9' of TRF which is active in accelerating the release of 1311 from the thyroid (Shibusawa, Yamamoto, Nishi, Abe & Tomic 1959). This TRF material is produced in the anterior hypothalamus of the dog and is found in the neurohypophysis, anterior hypothalamus, blood serum, urine and cerebrospinal fluid in diminishing order of concentration.…”

TRF Concentrations in Various Tissues Following Anterior Hypothalamic Lesions

“…in the neurohypophysis, and then passed into the cerebrospinal fluid and the blood stream to be excreted into urine (Shibusawa et al, 1959c). It has been demonstrated neither in the median and posterior hypothalamus, nor in other tissues, and body fluids.…”

“…It has been demonstrated neither in the median and posterior hypothalamus, nor in other tissues, and body fluids. It is an hitherto unknown hormone which controls the thyroid function by acting upon the adenohypophysis via the hypophyseal portal vein or systemic circulation (Shibusawa et al, 1959c). The concentration of this" hormone" in the anterior hypothalamus and the neurohypophysis varies chiefly depending on the balance between the production and release of TRF.…”