Studies on the toxicity of some carbamate fungicides in Drosophila melanogaster meig. (insecta, diptera)

Marchal‐Ségault, D.; Seugé, Jacqueline; Laugé, G.

doi:10.1016/0013-9351(85)90046-5

Cited by 6 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A method described earlier (Marchal-Segault et al, 1985) was followed to examine the effects' if any, of Nuvan and Dimecron on early development by examining hatchability of eggs. Briefly, flies were allowed to lay eggs on normal food for 0.5 h, followed by transfer of the eggs to normal food, 0.3% DMSO food and food with different concentrations of Nuvan and Dimecron.…”

Section: Hatchability Of Eggsmentioning

confidence: 99%

Comparative toxic potential of market formulation of two organophosphate pesticides in transgenic Drosophila melanogaster (hsp70-lacZ)

et al. 2005

View full text Add to dashboard Cite

This study investigated the working hypothesis that two widely used organophosphate pesticides; Nuvan and Dimecron, exert toxic effects in Drosophila. Transgenic D. melanogaster (hsp70-lacZ) was used as a model for assaying stress gene expression and AchE activity as an endpoint for toxicity and also to evaluate whether stress gene expression is sufficient to protect against toxic insult of the chemicals and to prevent tissue damage. The study was extended to investigate the effect of the pesticides on the life cycle and reproduction of the organism. The study showed that Nuvan affected emergence of the exposed flies more drastically than Dimecron and the effect was lethal at the highest tested concentration (0.075 ppm). While Nuvan at 0.0075 and 0.015 ppm concentrations affected reproduction of the flies significantly, the effect of Dimecron was significant only at 0.015 and 0.075 ppm. Nuvan-exposed third-instar larvae exhibited a 1.2-fold to 1.5-fold greater hsp70 expression compared to Dimecron at concentrations ranging from 0.0075 to 0.075 ppm following 12 and 18 h exposure. While maximum expression of hsp70 was observed in Nuvan-exposed third-instar larval tissues following 18 h exposure at 0.075 ppm, Dimecron at the same dietary concentration induced a maximum expression of hsp70 following 24 h exposure. Further, concomitant with a significant induction of hsp70, significant inhibition of AchE was observed following chemical exposure and temperature shock. Concurrent with a significant decline in hsp70 expression in Nuvan-exposed larvae after 48 h at 0.075 ppm, tissue damage was evident. Dimecron-exposed larvae exhibited a plateau in hsp70 induction even after 48 h exposure and moderate tissue damage was observed in these larvae. The present study suggests that Nuvan is more cytotoxic than Dimecron in transgenic Drosophila melanogaster.

show abstract

Section: Hatchability Of Eggsmentioning

confidence: 99%

Comparative toxic potential of market formulation of two organophosphate pesticides in transgenic Drosophila melanogaster (hsp70-lacZ)

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Flies were transferred to the fresh vials every alternate day and the number of dead flies was recorded till the last fly was dead [13].…”

Section: Survivorship Of Adult Fliesmentioning

confidence: 99%

“…To study the effect of captan on hatchability of eggs, an earlier described method was followed [13]. Briefly, flies were allowed to lay eggs on normal food for 0.5 h, followed by transferring the eggs to normal food and food containing different concentrations of captan (300 eggs per group).…”

Section: Hatchability Of Eggsmentioning

confidence: 99%

“…The effect of captan on the life span of adult flies was studied by exposing the adult flies to food mixed with different concentrations of captan from day 1 of their life cycle (50 flies/vial and 10 vials per group). Flies were transferred to the fresh vials every alternate day and the number of dead flies was recorded till the last fly was dead [13].…”

Section: Survivorship Of Adult Fliesmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of toxic potential of captan: Induction of hsp70 and tissue damage in transgenic drosophila melanogaster (hsp70‐lacZ) Bg⁹

Nazir

Mukhopadhyay

Saxena

et al. 2003

J Biochem & Molecular Tox

View full text Add to dashboard Cite

The study investigated the working hypothesis that a widely used fungicide captan exerts toxic effects on nontarget organisms. Transgenic Drosophila melanogaster (hsp70-lacZ) was used as a model by assaying stress gene expression as an endpoint for cytotoxicity and also to evaluate whether stress gene expression is sufficient enough to protect and to prevent tissue damage against toxic insult of the chemical. The study was further extended to understand the effect of the pesticide on development, life cycle, and reproduction of the organism and finally to evaluate a concentration of the chemical to be nontoxic to the organism. The study showed that (i) captan causes cytotoxicity at and above 0.015 ppm; (ii) at 0.0015 ppm captan, absence of hsp70 expression in the exposed organism was evaluated as the concentration referred to as no observed adverse effect level (NOAEL) for Drosophila; (iii) emergence pattern of flies was affected only at the highest concentration of captan by 4 days, while hatching and survivorship were unaffected even at this concentration; (iv) reproductive performance was significantly affected only at 125.0 and 1250.0 ppm captan, while in the lower dietary concentrations no such deleterious effects were observed; (v) at 1250.0 ppm, hsp70 failed to protect the cells from toxicant assault after 48 h exposure, thus leading to tissue damage as revealed by Trypan Blue staining. The present study shows the cytotoxic potential of captan and further reveals the application of stress genes in determining NOAEL and its expression as bioindicator of exposure to environmental contaminants.

show abstract

Chlorpyrifos-Induced hsp70 Expression and Effect on Reproductive Performance in Transgenic Drosophila melanogaster (hsp70-lacZ) Bg 9

Nazir

Mukhopadhyay

Saxena

et al. 2001

Arch. Environ. Contam. Toxicol.

View full text Add to dashboard Cite

Expression of hsp70 in the third-instar larval tissues of transgenic Drosophila melanogaster (hsp70-lacZ) following dietary exposure to organophosphate insecticide chlorpyrifos for various time intervals was investigated. Effect of the chemical on different developmental stages of the fly was also evaluated by looking at survivorship, hatchability, emergence, fecundity, fertility, and reproductive performance. Our results showed that the toxicant evokes profound cytotoxic effect as evidenced by dark blue staining in salivary gland, proventriculus, brain ganglia, and midgut in the lowest concentration of the chemical following 24 and 48 h of exposure. On the other hand, a significant increase (61%) in hsp70 expression in the above larval tissues was observed in the next higher concentration of the toxicant after 6 h exposure when compared with that of the lowest dose. A further increase in exposure time caused 100% larval mortality. Similarly, larvae exposed to higher concentrations of the toxicant, exhibited 100% mortality within 1 h of treatment. The insecticide caused a delay in emergence and a severe reduction in survivorship of the flies in a dose-dependent manner with 100% mortality within a day of exposure in the highest dosed group. A delay in emergence by 3 days was evident even in the lowest concentration of the chemical. A drastic effect of the chemical on hatchability was found in the highest dosed group with 100% embryonic mortality at post-16 stage of the embryo. Reproductive performance was significantly affected even in the lowest dosed group. The present study suggests that certain larval tissues of Drosophila, a nontarget organism, are vulnerable to chlorpyrifos as evidenced by hsp70 expression. Further, the adverse effect of the toxicant is reflected on various stages of development of the fly including reproductive performance.

show abstract

Studies on the toxicity of some carbamate fungicides in Drosophila melanogaster meig. (insecta, diptera)

Cited by 6 publications

References 5 publications

Comparative toxic potential of market formulation of two organophosphate pesticides in transgenic Drosophila melanogaster (hsp70-lacZ)

Comparative toxic potential of market formulation of two organophosphate pesticides in transgenic Drosophila melanogaster (hsp70-lacZ)

Evaluation of toxic potential of captan: Induction of hsp70 and tissue damage in transgenic drosophila melanogaster (hsp70‐lacZ) Bg⁹

Chlorpyrifos-Induced hsp70 Expression and Effect on Reproductive Performance in Transgenic Drosophila melanogaster (hsp70-lacZ) Bg 9

Contact Info

Product

Resources

About

Studies on the toxicity of some carbamate fungicides in Drosophila melanogaster meig. (insecta, diptera)

Cited by 6 publications

References 5 publications

Comparative toxic potential of market formulation of two organophosphate pesticides in transgenic Drosophila melanogaster (hsp70-lacZ)

Comparative toxic potential of market formulation of two organophosphate pesticides in transgenic Drosophila melanogaster (hsp70-lacZ)

Evaluation of toxic potential of captan: Induction of hsp70 and tissue damage in transgenic drosophila melanogaster (hsp70‐lacZ) Bg9

Chlorpyrifos-Induced hsp70 Expression and Effect on Reproductive Performance in Transgenic Drosophila melanogaster (hsp70-lacZ) Bg 9

Contact Info

Product

Resources

About

Evaluation of toxic potential of captan: Induction of hsp70 and tissue damage in transgenic drosophila melanogaster (hsp70‐lacZ) Bg⁹