Studies on the transition of the cristal membrane from the orthodox to the aggregated configuration. I: Topology of bovine adrenal cortex mitochondria in the orthodox configuration

Allmann, David W.; Wakabayashi, Takashi; Korman, Ephraim F.; Green, David E.

doi:10.1007/bf01516090

Cited by 47 publications

(6 citation statements)

References 15 publications

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“…Many researchers in fact interpret the cristae of the zona fasciculata to be vesicular, or tubulovesicular (Nussdorfer, 1986). The interpretation of Allmann et al (1970), however, is perhaps most accurate and these cristae are likely composed of vesicular regions connected by narrow tubules.…”

mentioning

confidence: 99%

Mitochondrial structure in steroid‐producing cells: Three‐dimensional reconstruction of human Leydig cell mitochondria by electron microscopic tomography

Prince

Buttle

2004

Anat. Rec.

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Mitochondria of human Leydig cells were reconstructed in three dimension utilizing the technique of electron microscopic tomography to obtain a better understanding of the topology of the internal membrane system and the relationship of these cristae to the inner boundary membrane (IBM). Cristae structure, in many respects, is consistent with previous tomographic studies from typical mitochondria, i.e., mitochondria from nonsteroid-producing cells. Cristae are diverse in form, with well-defined lamellar cristae interconnected to pleomorphic and tubular regions. Occasional fenestrations are present in the lamellar regions. Also consistent with other mitochondria studied by tomography, the openings of the cristae to the IBM (referred to as crista junctions) are roughly circular or elliptical and approximately 20 -25 nm in diameter. Morphological contact sites between the outer mitochondrial membrane and IBM are also present. Cristae membranes in these steroid-producing mitochondria are often found in close proximity to the IBM. Unique to steroid-producing mitochondria is a form of the cristae in which multiple lamellae are in very close apposition, previously defined as the lamellar association. Tomographic reconstructions of the lamellar association reveal that these well-organized membranes also open to the IBM via crista junctions. These regions of closely apposed lamellar cristae are also interconnected and display small tubular extensions from the lamellae. The current study is the first electron microscopic tomography study of mitochondria from steroid-producing cells. The results show the cristae interconnect to form an extensive internal membrane system, which is perhaps better termed the cristae compartment. This internal membrane system is notable due to the high surface area with few small openings to the IBM. Such a morphology is more analagous to the thylakoid membrane system of chloroplasts than the long-standing view of mitochondrial cristae. The significance of the lamellar association form of the cristae is unknown. Anat Rec Part A 278A: 454 -461, 2004.

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confidence: 99%

Mitochondrial structure in steroid‐producing cells: Three‐dimensional reconstruction of human Leydig cell mitochondria by electron microscopic tomography

Prince

Buttle

2004

Anat. Rec.

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“…The specimens of mitochondrial and microsomal fractions were fixed in 2% glutaraldehyde in isosmolar phosphate buffer followed by osmium tetroxide and em bedded in epon.araldite resin for electron microscopy according to Allamann et al [4] …”

Section: Tissue Preparationsmentioning

confidence: 99%

The Aromatizing Activity of Placental Mitochondrial and Microsomal Fractions

Aleem¹,

Valdivia²,

Colás³

1970

Gynecol Obstet Invest

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Examination with the electron microscope showed that mitochondrial and microsomal fractions from py human placenta were not homogeneous, despite their careful preparation by several methods. Repeated washing of the mitochondrial fraction, which substantially reduced its glucose-6-phosphatase activity, caused only a small decrease in the aromatizing activity of this fraction. In incubations with 4-androstene-3,17-dione, testosterone and 3Β-hydroxy-5-androstene-17-one, the ratio of 17Β-estradiol to estrone produced was greater with the microsomal ( > 2.00) than with the mitochondrial fraction (0.59–1.82) at each substrate level.

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“…Unlike WT mitochondria in which tubular cristae are stabilized in the “orthodox-configuration” (i.e., scalloped with alternating repeats of distension and constriction), most TASK-3 KO cristae are captured in an “aggregated configuration” (i.e., appearing as tubular/lamellar structures) 31,32 . Importantly, in adrenal mitochondria an orthodox-to-aggregated transition is indicative of increased mitochondrial ATP synthesis or Ca 2+ transport 33 .…”

Section: Resultsmentioning

confidence: 99%

“…First, a greater proportion of mitochondria in TASK-3 KO ZG cells have expanded intracristal spaces. In the adrenal cortex, the topology of the cristae membrane (involutions of the inner mitochondrial membrane) dynamically changes with the physiological state of the cell 32 . The condensed configuration characterized by tubular lamellae is more prevalent in TASK-3 KO mitochondria and is associated with (and presumably supports) an increased capacity to translocate Ca 2+ 33 .…”

Section: Discussionmentioning

confidence: 99%

Functional TASK-3–Like Channels in Mitochondria of Aldosterone-Producing Zona Glomerulosa Cells

et al. 2017

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Ca2+ drives aldosterone synthesis in the cytosolic and mitochondrial compartments of the adrenal zona glomerulosa (ZG) cell. Membrane potential across each of these compartments regulates the amplitude of the Ca2+ signal; yet, only plasma membrane ion channels and their role in regulating cell membrane potential have garnered investigative attention as pathological causes of human hyperaldosteronism. Previously, we reported that genetic deletion of TASK-3 channels from mice produces aldosterone excess in the absence of a change in the cell membrane potential of ZG cells. Here, we report using yeast two-hybrid, immunoprecipitation and electron microscopic analyses that TASK-3 channels are resident in mitochondria, where they regulate mitochondrial morphology, mitochondrial membrane potential (mitoVm) and aldosterone production. This study provides proof-of-principle that mitochondrial K+ channels, by modulating inner mitochondrial membrane morphology and mitoVm, have the ability to play a pathological role in aldosterone dysregulation in steroidogenic cells.

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Studies on the transition of the cristal membrane from the orthodox to the aggregated configuration. I: Topology of bovine adrenal cortex mitochondria in the orthodox configuration

Cited by 47 publications

References 15 publications

Mitochondrial structure in steroid‐producing cells: Three‐dimensional reconstruction of human Leydig cell mitochondria by electron microscopic tomography

Mitochondrial structure in steroid‐producing cells: Three‐dimensional reconstruction of human Leydig cell mitochondria by electron microscopic tomography

The Aromatizing Activity of Placental Mitochondrial and Microsomal Fractions

Functional TASK-3–Like Channels in Mitochondria of Aldosterone-Producing Zona Glomerulosa Cells

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