Studies on the translocation of noradrenaline‐containing vesicles in post‐ganglionic sympathetic neurones <i>in vitro</i>. Inhibition of movement by colchicine and vinblastine and evidence for the involvement of axonal microtubules

Banks, Peter A.; Mayor, D.; Mitchell, Mary; Tomlinson, David R.

doi:10.1113/jphysiol.1971.sp009544

Cited by 104 publications

(27 citation statements)

References 8 publications

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“…1. Noradrenaline in the segments was measured by the method of Haggendal (1963) described by Banks et al (1971a). Noradrenaline accumulation was always confined to segments P, to P3.…”

Section: Methodsmentioning

confidence: 99%

“…Antimitotic drugs, such as colchicine, which depolymerize axonal microtubules, also inhibit the intra-axonal movement of the storage vesicles (Dahlstrdm, 1968; Banks, Mayor, Mitchell & Tomlinson, 1971a; Banks, Mayor & Tomlinson, 1971b). Observations of this sort have led to the idea that microtubules are components of a system governing the intra-axonal migration of subcellular organelles.…”

Section: Introductionmentioning

confidence: 99%

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A correlation between the effects of anti‐mitotic drugs on microtubule assembly in vitro and the inhibition of axonal transport in noradrenergic neurones.

Banks¹,

Till²

1975

The Journal of Physiology

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SUMMARY1. Podophyllotoxin, colchicine and griseofulvin inhibit the intra-axonal movement of noradrenaline storage vesicles in cat hypogastric nerve/inferior mesenteric ganglion preparations maintained in vitro, cause the disappearance of axonal microtubules and inhibit the assembly of microtubules from tubulin in vitro. The order of potency for the three effects is podophyllotoxin > colchicine > griseofulvin.2. Lumicolchicine is without effect on the three parameters and does not interfere with the binding of tritiated colchicine to tubulin.3. Podophyllotoxin causes a more rapid loss of microtubules from axons than the same concentration of colchicine.4. The experiments provide strong evidence that microtubules are components of the system responsible for the intra-axonal migration of noradrenaline storage vesicles.

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“…1. Noradrenaline in the segments was measured by the method of Haggendal (1963) described by Banks et al (1971a). Noradrenaline accumulation was always confined to segments P, to P3.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A correlation between the effects of anti‐mitotic drugs on microtubule assembly in vitro and the inhibition of axonal transport in noradrenergic neurones.

Banks¹,

Till²

1975

The Journal of Physiology

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“…A preparation comprising the inferior mesenteric ganglion with attached hypogastric nerves, ligated 2 cm distal to the ganglion, was removed from anaesthetised cats (weight: 2.5-3.5 kg), which had received a normal diet, using an aseptic technique as described elsewhere [2]. The preparation was transferred immediately to either a two or three compartment culture chamber (Fig.l).…”

Section: Single Ligation Experimentsmentioning

confidence: 99%

“…Axonal transport was studied in vitro by measurement of the accumulation of materials proximal to a constriction applied to cat hypogastric nerves attached to the inferior mesenteric ganglion maintained in organ culture [2]. The effects of exposure of the axons to culture medium containing an elevated glucose concentration were studied.…”

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confidence: 99%

Axonal transport of noradrenaline, protein and glycoprotein in cat hypogastric nerves in vitro under conditions of high extracellular glucose

Tomlinson

1983

Diabetologia

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Summary.Inferior mesenteric ganglia with attached hypogastric nerves were removed from anaesthetised cats and maintained in Eagle's Minimal Essential Medium in specially designed organ culture chambers for 48 h at 37 ~ The chambers had removable barriers so that the fluid bathing the ganglia was separated from that bathing the nerve trunks by inert silicone grease seals. (3H)-fucose (1.25 ~tCi/ml) and (14C)-leucine (0.5 .aCi/ml) were added to the fluid bathing the ganglia at the start of the experiment. At this time, tight ligatures were applied to the hypogastric nerves so that the incorporated isotopes accumulated proximal to the constrictions due to interrupted axonal transport. The nerve trunks were bathed in medium containing glucose at either 5.5 or 25 retool/1. At the end Of the incubation period, the accumulations, proximal to the constrictions, of incorporated leucine and fucose and of noradrenaline were measured. The high glucose medium caused a 27.5% (p<0.001) reduction in the accumulation of fucose and a 19.2% (p< 0.01) reduction in the accumulation of leucine. The incorporation of these tracers in the ganglia was unaffected by exposure of the nerve trunks to high glucose. The accumulation of noradrenaline proximal to the constrictions was also unaffected. Further experiments were performed to show that the reduced accumulations of fucose and leucine under conditions of high glucose were not due to loss of isotope from macromolecules in the axons nor to the small increase in osmotic pressure effected by addition of extra glucose to the culture medium. It is therefore suggested that elevation of extracellular glucose caused an impairment of axonal transport of incorporated leucine and fucose.

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“…Rapid axonal transport of adrenergic vesicles is dependent upon the integrity of the microtubular system (Dahlstrom, 1970;Banks et al, 1971). The ability of crude or partially purified rat brain tubulin to polymerize was carefully studied by Weisenberg (1972).…”

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confidence: 99%

The EFFECTS OF THE CALCIUM IONOPHORE, A23187, ON THE AXOPLASMIC TRANSPORT OF DOPAMINE Β‐HYDROXYLASE

Esquerro¹,

Garcı́a²,

Sánchez‐García³

1980

British J Pharmacology

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1The effects of the ionophore, A23187, on the intra-axonal transport of dopamine f3-hydroxylase (DBH) were investigated in the cat hypogastric nerve-inferior mesenteric ganglion preparation by monitoring, in vitro, the enzyme accumulation above a ligature, 2 to 2.5 cm distal to the ganglion.2 DBH accumulation in the proximal segment immediately above the ligature (P,) increased linearly up to 6 h, during incubation in normal Krebs solution at 37°C. The ionophore, A23187, interfered with the enzyme accumulation, but did not modify the previously accumulated DBH activity present in P1. 3 The blocking effects of A23187 on DBH transport were greatly impaired in the absence of extracellular calcium ions; an excess of calcium in the bathing solution (7.5 mM) itself blocked the enzyme transport by 50%.4 A23187 did not significantly modify the levels of adenosine triphosphate (ATP) in the segments P1 and P2 of the nerve proximal to the ligature. 5 Nerves incubated in an A23187-containing medium showed many mitochondria of normal shape and fine structure; however, typical microtubules or filaments were not seen in these preparations. 6 The results suggest that the ionophore A23187, by considerably raising the axoplasmic ionized calcium levels, interferes with the assembling of microtubules. In this manner, the ionophore would inhibit the transport of adrenergic vesicles and therefore of DBH along the axon. The results also provide additional evidence in favour of the view that for the transport system to work adequately, it is necessary to maintain the intra-axoplasmic ionized calcium concentration between certain critical levels.

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Studies on the translocation of noradrenaline‐containing vesicles in post‐ganglionic sympathetic neurones in vitro. Inhibition of movement by colchicine and vinblastine and evidence for the involvement of axonal microtubules

Cited by 104 publications

References 8 publications

A correlation between the effects of anti‐mitotic drugs on microtubule assembly in vitro and the inhibition of axonal transport in noradrenergic neurones.

A correlation between the effects of anti‐mitotic drugs on microtubule assembly in vitro and the inhibition of axonal transport in noradrenergic neurones.

Axonal transport of noradrenaline, protein and glycoprotein in cat hypogastric nerves in vitro under conditions of high extracellular glucose

The EFFECTS OF THE CALCIUM IONOPHORE, A23187, ON THE AXOPLASMIC TRANSPORT OF DOPAMINE Β‐HYDROXYLASE

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