Studies on Thermal Denaturation of DNA from Various Chlamydiae

Gerloff, Robert K.; Ritter, Daniel B.; Watson, Rex O.

doi:10.1093/infdis/121.1.65

Cited by 16 publications

(5 citation statements)

References 19 publications

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“…2). The base composition of the ORF was 43% GϩC, in close agreement with the expected A-T-rich composition of chlamydial sequences (7,12). Sliding nucleotide composition analysis (analysis length, 20; offset, 2) indicated that the highest GC% existed between nt 850 and 1000, a region rich in predicted proline residues (Textco Inc.).…”

supporting

confidence: 78%

The glycyl-tRNA synthetase of Chlamydia trachomatis

Wagar¹,

Giese²,

Yasin³

et al. 1995

J Bacteriol

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Aminoacyl-tRNA synthetases specifically charge tRNAs with their cognate amino acids. A prototype for the most complex aminoacyl-tRNA synthetases is the four-subunit glycyl-tRNA synthetase from Escherichia coli, encoded by two open reading frames. We examined the glycyl-tRNA synthetase gene from Chlamydia trachomatis, a genetically isolated bacterium, and identified only a single open reading frame for the chlamydial homolog (glyQS). This is the first report of a prokaryotic glycyl-tRNA synthetase encoded by a single gene.

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supporting

confidence: 78%

The glycyl-tRNA synthetase of Chlamydia trachomatis

Wagar¹,

Giese²,

Yasin³

et al. 1995

J Bacteriol

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“…In the genus Chlamydia, interspecies DNA-DNA homology is less than 10%, while intraspecies DNA-DNA homology is almost 100% (12), although the mouse pneumonitis strain which is the mouse biovar of C. trachomatis showed only 30 to 60% DNA-DNA homology with other strains of the same species (29). The DNA-DNA homology of C. psittaci was, however, estimated for only two avian-related strains, California 10 (designated Frt-Hu/CallO in the present study) and 6BC (Prk/6BC in the present study) (12), despite the isolation of C. psittaci from a variety of animals and birds and despite a wide range of guanine and cytosine content (40 to 44 mol% G+C) (9). Immunological and biological analyses indicated phenotypic heterogeneity of C. psittaci (14,27).…”

contrasting

confidence: 53%

Genetic diversity of avian and mammalian Chlamydia psittaci strains and relation to host origin

Fukushi

Hirai

1989

J Bacteriol

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Genetic relationships were reported for Chlamydia psittaci derived from psittacine birds, pigeons, turkeys, humans, cats, muskrats, cattle, and sheep and for C. trachomatis, including representative strains of the three biovars, through physical analysis of genomic DNA including DNA fingerprinting with restriction endonuclease Sall, DNA-DNA hybridization in solution with S1 nuclease, and Southern analysis with genomic DNA probes. A total of 26 strains were divided into four groups of C. psittaci and two groups of C. trachomatis, on the basis of DNA fingerprints. The six groups of Chlamydia spp. were related to host origin: two avian groups (Avl and Av2), one feline and muskrat group (Fel), one ruminant group (Rul), one C. trachomatis biovars trachoma and lymphogranuloma group (CtHu), and one C. trachomatis mouse biovar group (CtMo), although an ovine abortion strain belonged to the avian group Av2. DNA-DNA hybridization assay and Southern analysis with genomic DNA probes indicated three DNA homology groups in the genus Chlamydia: an avian-feline group (groups Avl, Av2, and Fel), a ruminant group (group Rul), and a C. trachomatis group (groups CtHu and CtMo). Furthermore, the Southern analysis indicated that the homologous sequences (DNA homology of at least 14%) within the avian-feline group were distributed along the whole genome, whereas the homologous sequences (DNA homology of less than 24%) among the three DNA homology groups were localized in distinct regions of the genome DNA. These results suggest that Chlamydia spp. are derived from a common ancestor and have diverged into various groups showing restricted host ranges as a natural characteristic and that the species C. psittaci should be differentiated into groups related to host origin and DNA homology.The genus Chiamydia comprises two species, Chiamydia psittaci and Chlamydia trachomatis (23). Differentiation of the two species is based on natural hosts, inclusion morphology, glycogen in inclusions, and folate biosynthesis (23). In the genus Chlamydia, interspecies DNA-DNA homology is less than 10%, while intraspecies DNA-DNA homology is almost 100% (12), although the mouse pneumonitis strain which is the mouse biovar of C. trachomatis showed only 30 to 60% DNA-DNA homology with other strains of the same species (29). The DNA-DNA homology of C. psittaci was, however, estimated for only two avian-related strains, California 10 (designated Frt-Hu/CallO in the present study) and 6BC (Prk/6BC in the present study) (12), despite the isolation of C. psittaci from a variety of animals and birds and despite a wide range of guanine and cytosine content (40 to 44 mol% G+C) (9). Immunological and biological analyses indicated phenotypic heterogeneity of C. psittaci (14,27). Recent analyses of DNA fingerprints also showed differences among several strains of C. psittaci (1,18,28).We have reported a series of studies for establishing a systematic classification of C. psittaci, such as immunological typing with monoclonal antibodies (8) and immunoblotting analysis ...

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“…The latter DNA did not reassociate at all with DNA from C. psittaci (meningopneumonitis strain) (162). These and similar results reported by Gerloff et al (34,35) indicate that C. trachomatis of human origin shares only part of its DNA genome with the murine C. psittaci organisms. This also suggests that C. psittaci and C. trachomatis evolved from two different ancestors (67,160).…”

Section: Classification Of the Chlamydiae Current Notions In The Classupporting

confidence: 83%

The chlamydia: molecular biology of procaryotic obligate parasites of eucaryocytes.

Becker¹

1978

Microbiological Reviews

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Studies on Thermal Denaturation of DNA from Various Chlamydiae

Cited by 16 publications

References 19 publications

The glycyl-tRNA synthetase of Chlamydia trachomatis

The glycyl-tRNA synthetase of Chlamydia trachomatis

Genetic diversity of avian and mammalian Chlamydia psittaci strains and relation to host origin

The chlamydia: molecular biology of procaryotic obligate parasites of eucaryocytes.

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