Studies on thermodynamic nature of steroselectivity for ruthenium(II) polypyridyl complex binding to DNA

Xu, Hong; Zhu, Qianqian; Lu, Jun; Chen, Xiaojuan; Xiao, Jie; Liu, Zhigang; Chen, Siping; Tong, Ming‐Liang; Ji, Liang‐Nian; Liang, Yi

doi:10.1016/j.inoche.2010.03.025

Cited by 20 publications

(4 citation statements)

References 28 publications

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“…Although these binding constants are not the same as those obtained from UV−vis absorption and fluorescence spectroscopic studies, all the three sets of binding constants indicated that the two enantiomers could tightly bind to CT-DNA, and the Δ -form interacted with DNA more strongly than the Λ -form did. The binding stoichiometry n obtained for Δ - and Λ -enantiomers were 1.62 and 1.57 base pairs, respectively, which are similar to those of some other Ru(II) polypyridine complexes. , The difference among the three sets of binding constants and binding stoichiometry should originate from the different experimental techniques used and the different calculation modes applied, which were also reported previously …”

Section: Resultssupporting

confidence: 84%

Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex

et al. 2022

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A pair of ruthenium(II) complex enantiomers, Δ- and Λ-[Ru(bpy)2MBIP]2+ (bpy = 2,2′-bipyridine, MBIP = 2-(3-bromophenyl)imidazo[5,6-f]phenanthroline), were designed, synthesized, and characterized. Comparative studies between the enantiomers on their binding behaviors to calf thymus DNA (CT-DNA) were conducted using UV−visible, fluorescence, and circular dichroism spectroscopies, viscosity measurements, isothermal titration calorimetry, a photocleavage experiment, and molecular simulation. The experimental results indicated that both the enantiomers spontaneously bound to CT-DNA through intercalation stabilized by the van der Waals force or the hydrogen bond and driven by enthalpy and that Δ-[Ru(bpy)2MBIP]2+ intercalated into DNA more deeply than Λ-[Ru(bpy)2MBIP]2+ did and exhibited a better DNA photocleavage ability. Molecular simulation further indicated that Δ-[Ru(bpy)2MBIP]2+ more preferentially intercalated between the base pairs of CT-DNA to the major groove, and Λ-[Ru(bpy)2MBIP]2+ more favorably intercalated to the minor groove. These research findings should be very helpful to the understanding of the stereoselectivity mechanism of DNA-bindings of metal complexes, and be useful for the design of novel metal-complex-based antitumor drugs with higher efficacy and lower toxicity.

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Section: Resultssupporting

confidence: 84%

Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex

et al. 2022

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“…Protein/peptide interactions with polymers and nanoparticles Nucleic acid interactions other than with proteins Enzyme activity and kinetics Other unclassified studies (including, e.g.…”

Section: Introductionmentioning

confidence: 99%

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

Ghai

Falconer

Collins

2011

J of Molecular Recognition

167

108

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Isothermal titration calorimetry (ITC) is a biophysical technique for measuring the formation and dissociation of molecular complexes and has become an invaluable tool in many branches of science from cell biology to food chemistry. By measuring the heat absorbed or released during bond formation, ITC provides accurate, rapid, and label-free measurement of the thermodynamics of molecular interactions. In this review, we survey the recent literature reporting the use of ITC and have highlighted a number of interesting studies that provide a flavour of the diverse systems to which ITC can be applied. These include measurements of protein-protein and protein-membrane interactions required for macromolecular assembly, analysis of enzyme kinetics, experimental validation of molecular dynamics simulations, and even in manufacturing applications such as food science. Some highlights include studies of the biological complex formed by Staphylococcus aureus enterotoxin C3 and the murine T-cell receptor, the mechanism of membrane association of the Parkinson's disease-associated protein α-synuclein, and the role of non-specific tannin-protein interactions in the quality of different beverages. Recent developments in automation are overcoming limitations on throughput imposed by previous manual procedures and promise to greatly extend usefulness of ITC in the future. We also attempt to impart some practical advice for getting the most out of ITC data for those researchers less familiar with the method.

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“…The binding stoichiometry was dependent on the identity of the metal ion, and a noteworthy feature of this study was that it employed isothermal titration calorimetry (ITC) to study the thermodynamics of the binding interaction (65). ITC has been employed to observe the thermodynamics of DNA binding with metal complexes (66)(67)(68), and its applicability to binding of metals and metal complexes with DNA is expected to increase (65). It is being employed in studying alternative base pairing mediated by metal ions, which is addressed in a later section.…”

Section: Dna-metal Complex Interactionsmentioning

confidence: 99%

DNA-bound metal ions: recent developments

Morris

2014

Biomolecular Concepts

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Abstract:The affinity of metal ions for DNA is logical considering that the structure of DNA includes a phosphate backbone with a net-negative charge, a deoxyribose sugar with O atoms, and purine and pyrimidine bases that contain O and N atoms. DNA-metal ion interactions encompass a large area of research that ranges from the most fundamental characterization of DNA-metal ion binding to the role of DNA-bound metal ions in disease and human health. Alternative DNA base pairing mediated by metal binding is also being investigated and manipulated for applications in logic gates, molecular machines, and nanotechnology. This review highlights recent work aimed at understanding interactions of redox-active metal ions with DNA that provides a better understanding of the mechanisms by which various types of oxidative DNA damage (strand breakage and base modifications) occur. Antioxidants that mitigate oxidative DNA damage by coordinating metal ions that produce reactive oxygen species are addressed, as well as recent work on the effect of DNAmetal ion interactions and the efficacy of quinolone-based antibacterial drugs. Recent advances in metal-mediated base pairing that triggers conformational changes in DNA structure for use as selective metal ion sensors and novel nanotechnology applications are also included.

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Studies on thermodynamic nature of steroselectivity for ruthenium(II) polypyridyl complex binding to DNA

Cited by 20 publications

References 28 publications

Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex

Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

DNA-bound metal ions: recent developments

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