Studies on tissue cultures of the genus Cinchona L.

Koblitz, H.; Koblitz, D.; Schmauder, H.‐P.; Gröger, D.

doi:10.1007/bf00270175

Cited by 22 publications

(3 citation statements)

References 3 publications

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“…This work led to the formulation of a medium (C19) containing levels of 2,4-D (0.5 mg 11) and BA (0.1 mg 1') in which growth and alkaloid production were maintained over an extended period of culture. The levels of alkaloid produced de tovo by cells growing in this medium compared favourably with those found by other workers using suspension cultures of C. ledgeriana (2) and C. pubescens (3). Although in callus it has been found that the level and type of alkaloid present (4, 5) may be manipulated by altering the amounts of plant growth regulators present, no attempt has previously been made to optimise their effect.…”

Section: Introductionsupporting

confidence: 74%

“…Cultures of Cinchona have been established in a number of laboratories (2)(3)(4)(5)(6)(7)(8)(9)(10)(11); methods for the multiplication of shoots (2) and the regeneration of whole plants from single shoot tips (3) have been developed. In the initial work on the production of alkaloids in culture (2, 4), significant production was found in organised leaf cultures (2) but not in unorganised suspension cultures (4).…”

Section: Introductionmentioning

confidence: 99%

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Cell Suspension Cultures ofCinchona ledgeriana; I. Growth and Quinoline Alkaloid Production

Robins¹,

Payne²,

Rhodes³

1986

Planta Med

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Callus and suspension cultures of Cinchona /edgeriana were established from sterile shoot segments in a medium containing 2.0 mg L' dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg Li benzyladeniae (BA). The production of alkaloids in these suspension cultures growing under standard conditions was stimulated from 0.01 to 4 mg L' by lowering the concentrations of 2, 4-D and BA in the medium to 0.5 and 0.1 mg Li, respectively. Cell suspension cultures of C. led geriana adapted to the low hormone medium released up to 75 % of their total alkaloid into the culture medium, The qualitative composition of the alkaloid mixture produced by the initial cultures resembled the explant tissue from which it was derived in having cinchonine, the precursor of quinidine as the major alkaloid constituent. Manipulation of the culture led not only to an increase in the total alkaloid formed but also to a relative increase in the formation of cinchonidine, the precursor having the stereochemical structure of quinine.

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Section: Introductionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Cell Suspension Cultures ofCinchona ledgeriana; I. Growth and Quinoline Alkaloid Production

Robins¹,

Payne²,

Rhodes³

1986

Planta Med

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“…Krikorian et al (5) reported the aseptic micropropagation of C. ledgeriana shoot cultures and Noerhadi (6) the establishment of cinchona callus. Koblitz et al (7) reported an in vitro clonal mass propagation procedure for C. ledgeriana and C. succirubra shoots derived from plant shoot-tips. The rooting capacity of shoots on a zeatin containing medium was higher than those cultivated on BA.…”

Section: Introductionmentioning

confidence: 99%

Effects of Age and Growth Regulators on Growth and Alkaloid Production inCinchona ledgerianaLeaf-Shoot Organ Cultures

Chung

Staba

1987

Planta Med

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CINCHONA LEDGERIANA Moens et Trimen leaf-shoot organ cultures established and maintained on Murashige and Skoog's medium containing benzyladenine (BA 5 mg/l) were used to study the effects of age and growth regulators on alkaloid production. The total and individual alkaloid content of the leaf-shoot organ cultures increased with age and resembled closely that of the 1-year-old plant which favored quinine production. The 32-week-old tissue cultures contained the same amount of alkaloid as that of the 1-year-old plant (350 mg%). Quinine production was favored by the presence of benzyladenine (5 mg/l), gibberellic acid (5 mg/l) and/or shoots. Quinidine production was high in the presence of indole-3-acetic acid (5 mg/l), the absence of benzyladenine, and/or the presence of roots. High concentrations of abscisic acid and mefluidide inhibited growth and alkaloid production.

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Alkaloid Production from Cinchona Cell and Organ Systems

Staba

1988

Genetic Manipulation of Woody Plants

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Studies on tissue cultures of the genus Cinchona L.

Abstract: A procedure for the in vitro clonal mass propagation of shoots and plants of two Cinchona species derived from single shoot tips is described. Special attention is given to rooting problems of the shoots under in vitro conditions.

Cited by 22 publications

References 3 publications

Cell Suspension Cultures ofCinchona ledgeriana; I. Growth and Quinoline Alkaloid Production

Cell Suspension Cultures ofCinchona ledgeriana; I. Growth and Quinoline Alkaloid Production

Effects of Age and Growth Regulators on Growth and Alkaloid Production inCinchona ledgerianaLeaf-Shoot Organ Cultures

Alkaloid Production from Cinchona Cell and Organ Systems

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