Studies on tissue expression of HCV proteins (NS3 and C) in chronic hepatitis C using the ImmunoMax technique

Kasprzak, Aldona; Biczysko, W; Adamek, Agnieszka; Wysocki, Jacek; Zabel, Maciej; Jurczyszyn, Donata; Chmielewski, Marcin; Surdyk-Zasada, Joanna

doi:10.1080/00365520310008818

Cited by 14 publications

(10 citation statements)

References 4 publications

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“…The observed by us lack of correlation between HCV protein content on one hand and grading and staging of the process on the other reflects probably the very mild histological lesions in children. No such correlations have been noted also in adult patients with HCV infection, in whom inflammatory lesions and advancement of fibrosis were more pronounced than in children with hepatitis C (26, 32). Our studies have disclosed no correlation also between HCV protein content in situ and serum levels of HCV RNA, even if such a correlation has previously been shown by others for protein C (24).…”

Section: Discussionmentioning

confidence: 98%

Intracellular localization of NS3 and C proteins in chronic hepatitis C

Kasprzak¹,

Seidel²,

Biczysko

et al. 2005

Liver International

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Section: Discussionmentioning

confidence: 98%

Intracellular localization of NS3 and C proteins in chronic hepatitis C

Kasprzak¹,

Seidel²,

Biczysko

et al. 2005

Liver International

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“…Liao et al (2011) and Kasprzak et al (2004) reported that tissue expression of NS3 protein does not correlate with histological grade or stage of liver tissues or clinical features in patients with CHC. In our study, we did not find correlation between the number of cells expressing HCV NS3 protein and ALT, AST and METAVIR inflammatory grade and fibrosis stage.…”

Section: Discussionmentioning

confidence: 99%

Extra-hepatic infection of hepatitis C virus in the colon tissue and its relationship with hepatitis C virus pathogenesis

Hetta

Mekky

Khalil

et al. 2016

Journal of Medical Microbiology

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Extra-hepatic compartments might contribute to hepatitis C virus (HCV) persistence and extrahepatic manifestations. Therefore, we investigated HCV infection in colonic tissue in patients with chronic hepatitis C (CHC) and its relationship with HCV pathogenesis. Colonic biopsies were collected from three groups with CHC infection: treatment naïve (TN; n=12), non-responders (NR; n=10) to anti-HCV therapy (pegylated interferon-a and ribavirin) and sustained virologic response (SVR; n=10) and from a fourth healthy control group (n=10). Liver biopsies were examined to assess inflammation and fibrosis. HCV infection and colonic T regulatory (T reg ) frequency were detected by immunohistochemistry. HCV core and NS3 proteins were detected in B cells and macrophage/monocytes of 42 % and 25 % of TN and 50 % and 30 % of NR, respectively, but not in SVR or control group. The numbers of cells expressing HCV proteins were positively correlated with both HCV viral load and colonic T reg frequency. A significant negative correlation between HCV-expressing cells with both liver inflammation and fibrosis was identified. Our study provides evidence that HCV can infect B cells and macrophages of the colon. The correlations between HCV infection in colonic tissue and HCV viral load and liver pathology underline the significance of this extra-hepatic infection in HCV pathogenesis and response to therapy.

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“…It is thought that these subviral particles, which are produced at concentrations several logs above that of infectious virus particles, absorb neutralizing antibody and trigger immunological tolerance, both of which promote virus persistence in the blood. Moreover, in patients with CLD, there does not seem to be any correlation between intrahepatic HBsAg expression patterns and inflammatory infiltrates [16,17], nor have HBsAg specific T cell clones been isolated from such patients [18]. In addition, T cell sensitization to HBsAg in acute and chronic HBV infection is usually undetectable [19],…”

Section: Hepatitis B Surface Antigen (Hbsag)mentioning

confidence: 99%

Pathogenesis of Hepatitis B Virus Associated Chronic Liver Disease

Feitelson¹

2018

Liver Cancer

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Hepatitis B virus (HBV) infection is associated with chronic liver diseases (CLD), which progress from hepatitis to fibrosis, cirrhosis, and finally hepatocellular carcinoma (HCC) over 30-50 years. The pathogenesis of CLD is immune mediated, which is characterized by persistent immune responses against virus infected hepatocytes. During bouts of CLD, the virus gene encoding the hepatitis B x antigen (HBx) is increasingly found integrated at multiple sites within the human genome. Many of these integrated templates express HBx, which is a trans-regulatory protein that supports virus gene expression and replication on one hand, but also alters patterns of gene expression in the infected cell. HBx alters gene expression by constitutively activating signal transduction pathways in the cytoplasm and promoting epigenetic mediated changes in the expression of cellular genes. In doing so, HBx contributes to the persistence of virus infected cells and to the pathogenesis of CLD by triggering multiple hallmarks which are characteristic of cancer.

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Studies on tissue expression of HCV proteins (NS3 and C) in chronic hepatitis C using the ImmunoMax technique

Cited by 14 publications

References 4 publications

Intracellular localization of NS3 and C proteins in chronic hepatitis C

Intracellular localization of NS3 and C proteins in chronic hepatitis C

Extra-hepatic infection of hepatitis C virus in the colon tissue and its relationship with hepatitis C virus pathogenesis

Pathogenesis of Hepatitis B Virus Associated Chronic Liver Disease

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