Studies on Transduction Process by SPP1 Phage

Ferrari, Eugenio; Canosi, Umberto; Galizzi, Alessandro; Mazza, G.

doi:10.1099/0022-1317-41-3-563

Cited by 21 publications

(21 citation statements)

References 19 publications

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“…Not only phage DNA but also B. subtilis chromosomal and plasmid DNAs can be packaged into SPP1 particles, although with very low efficiency. Therefore, the virulent SPP1 is also a transducing phage (4,7,33). The molecular weight of transducing DNA contained in SPP1 particles is identical to that of infective phage DNA.…”

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confidence: 99%

Plasmid transduction by Bacillus subtilis bacteriophage SPP1: effects of DNA homology between plasmid and bacteriophage

Deichelbohrer¹,

Alonso²,

Lüder³

et al. 1985

J Bacteriol

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Plasmid transduction by Bacillus subtilis bacteriophage SPP1: effects of DNA homology between plasmid and bacteriophage

Deichelbohrer¹,

Alonso²,

Lüder³

et al. 1985

J Bacteriol

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“…Phage PBSl was used as described by Moir et al (1979). Phage SPPl was used as described by Ferrari et al (1978) except that vegetative cells were used, rather than spores, to prepare lysates.…”

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Properties of a Mutant of Bacillus subtilis 168 in which Spore Germination is Blocked at a Late Stage

Warburg

Moir

1981

Microbiology

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A mutation, gerJ50, causing defective spore germination has been located between lys-1 and trpC2 on the Bacillus subtilis map and is 92% cotransduced with trpC2 by phage PBS1.Spores of strains containing gerJ50 will respond to germinants but the fall in absorbance is only 60% of that of the wild-type. During germination, mutant spores reach only an intermediate phase-grey stage, in contrast to those of the wild-type which become dark. The germination response, measured by the release of dipicolinic acid and the loss of resistance to heat, chloroform, octanol or toluene, is normal. The release of hexosamine-containing fragments from mutant spores is incomplete but has similar kinetics to that of wild-type spores. The mutant spores are more sensitive to heating at 90 OC which may point to a structural abnormality, but there is no evidence of a spore coat deficiency from electron microscopy of thin sections or freeze fractures or from the chemical resistances of the spores. Thus, the germination of mutant spores is initiated normally, but blocked at a late stage.

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“…Generalized transduction by SPP 1 has been repeatedly demonstrated (Ferrari et al, 1978;Lencastre & Archer, 1980;Yasbin & Young, 1974). We have found that lysates of 41c, 22a, p15 and SF6 share this capacity.…”

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Homology between Phages SPP1, 41c, 22a, 15 and SF6 of Bacillus subtilis

Santos

Lencastre

Archer

1984

Journal of General Virology

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SUMMARYBacteriophages SPP 1, 4 lc, 22a, p 15 and SF6 of Bacillus subtilis share a common and specific host receptor site for adsorption. Experiments described here have established the relatedness between these phages. They were indistinguishable on the basis of hostrange, plating efficiency, various growth parameters and serological properties. In addition, they shared the ability to carry out generalized transduction. They could be differentiated, however, by the restriction patterns of their DNAs, with the exception of 41c and 22a, which seemed to be identical. Recombination between 41c and SPP1 was demonstrated by transfection with mixed digests of their DNAs.A mutation conferring resistance to infection by bacteriophage SPP1 (pha-2) was recently mapped in the chromosome of Bacillus subtilis 168 (Santos et al., 1983). Out often other bacteriophages tested, only 41c, 22a, pl 5 and SF6 failed to infect pha-2 strains. Although these phages share some morphological characteristics with SPP 1, until now no evidence has been presented as to their possible relatedness. In order to clarify the specificity of the pha-2 mutation, a comparative study of these five phages was undertaken. SP01, a phage unrelated to SPP1 (Hemphill & Whiteley, 1975), was included as a control.Bacterial strains used are listed in (Okubo et al., 1964) were propagated on B. subtilis 168T + as described previously (Santos et al., 1983).Lysates were treated with 100 gg/ml DNase I at 37 °C for 30 min, before use in transduction experiments.M broth and M soft agar (Santos et al., 1983) were used for growth of bacteria, preparation of phage lysates and plating. SPP1, 41c, 22a, p15 and SF6 exhibit the same plaque morphology. They all form large, clear plaques, sometimes surrounded by a turbid halo, when plated on an appropriate strain. The Origin/reference

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Studies on Transduction Process by SPP1 Phage

Cited by 21 publications

References 19 publications

Plasmid transduction by Bacillus subtilis bacteriophage SPP1: effects of DNA homology between plasmid and bacteriophage

Plasmid transduction by Bacillus subtilis bacteriophage SPP1: effects of DNA homology between plasmid and bacteriophage

Properties of a Mutant of Bacillus subtilis 168 in which Spore Germination is Blocked at a Late Stage

Homology between Phages SPP1, 41c, 22a, 15 and SF6 of Bacillus subtilis

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