Studies on Transmissible Gastroenteritis in Pig in Japan

Sasahara, J; Harada, K.; Hayashi, Shigeo; Watanabe, Mamoru

doi:10.1292/jvms1939.20.1

Cited by 13 publications

(10 citation statements)

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“…Viruses. The virulent Shizuoka strain (Sasahara et al, 1958) of TGE virus was used for oral inoculation of pigs. The strain had been passaged 17 times in piglets and stored at -80°C in the form of a 10% suspension of infected small intestine.…”

Section: Methodsmentioning

confidence: 99%

Effects of ambient temperatures on clinical and immune responses of pigs infected with transmissible gastro-enteritis virus

Shimizu

1979

Veterinary Microbiology

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Shimizu, M. and Shimizu, Y., 1979. Effects of ambient temperatures on clinical and immune responses of pigs infected with transmissible gastroenteritis virus. Vet. Microbiol., 4: 109--116.Two-to three-months-old pigs infected with transmissible gastroenteritis (TGE) virus showed no clinical response when housed at 30°C, but comparable infected pigs exposed to temperature changes from 30°C to 4°C following infection showed typical signs of TGE. Development of TGE-specific immune responses, as measured by blastogenic response of tissue lymphocytes, occurred at 3 days post-inoculation (DPI) in pigs held at 30°C, but not until 7 DPI in infected pigs held under the adverse conditions. Immunosuppression with corticosteroids resulted in a fall in circulatory T cells, lowering of non-specific blastogenic response of circulatory lymphocytes, and clinical signs of disease when immunosuppressed pigs were infected with TGE virus and held at 30°C. It is suggested that clinical responses to TGE virus infection may be affected by the influence of ambient temperatures on the immune responses of pigs.

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Section: Methodsmentioning

confidence: 99%

Effects of ambient temperatures on clinical and immune responses of pigs infected with transmissible gastro-enteritis virus

Shimizu

1979

Veterinary Microbiology

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“…TGEV was isolated for the first time in 1946 [8]. Japan and England reported the disease in 1956 and 1957 [12,31]. The virus replicates in the cytoplasm of mature absorptive epithelial cells present on the tips of the villi in the small intestine.…”

Section: Introductionmentioning

confidence: 99%

Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus

et al. 2013

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Porcine transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PDEV) can cause severe diarrhea in pigs. Development of effective vaccines against TGEV and PEDV is one of important prevention measures. The spike (S) protein is the surface glycoprotein of TGEV and PEDV, which can induce specific neutralization antibodies and is a candidate antigen for vaccination attempts. In this study, the open reading frames of the TGEV S1 protein and in addition of the S or S1 proteins of PEDV were inserted into the eukaryotic expression vector, pIRES, resulting in recombinant plasmids, pIRES-(TGEV-S1-PEDV-S1) and pIRES-(TGEV-S1-PEDV-S). Subsequently, 6–8 weeks old Kunming mice were inoculated with both DNA plasmids. Lymphocyte proliferation assay, virus neutralization assay, IFN-γ assay and CTL activity assay were performed. TGEV/PEDV specific antibody responses as well as kinetic changes of T lymphocyte subgroups of the immunized mice were analyzed. The results showed that the recombinant DNA plasmids increased the proliferation of T lymphocytes and the number of CD4+ and CD8+ T lymphocyte subgroups. In addition, the DNA vaccines induced a high level of IFN-γ in the immunized mice. The specific CTL activity in the pIRES-(TGEV-S1-PEDV-S) group became significant at 42 days post-immunization. At 35 days post-immunization, the recombinant DNA plasmids bearing full-length S genes of TGEV and PEDV stimulated higher levels of specific antibodies and neutralizing antibodies in immunized mice.

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“…Transmissible gastroenteritis (TGE) is a widespread viral diarrhea of swine (4,5,14,17) in which the small intestine is the main, if not the only, target organ of the virus. This was shown in previous studies (7) on the growth of TGE virus in the infected pig in which the virus replicated to highest titers in the jejunum with somewhat smaller quantities being produced in the duodenum and ileum.…”

Section: Introductionmentioning

confidence: 99%

Transmissible gastroenteritis of swine: Virus-intestinal cell interactions I. Immunofluorescence, histopathology and virus production in the small intestine through the course of infection

Pensaert

Haelterman

Burnstein

1970

Archiv f Virusforschung

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A sequential study of the virus-intestinal cell interactions in the small intestinal mucosa of intact pigs and surgically isolated loops of jejunum was made using the Purdue strain of TGE virus. The different phases of the infection were examined by immunofluorescence, histopathology and measurement of virus production. Viral replication occurred only in the cytoplasm of the absorptive cells on the villi and resulted in cellular degeneration and desquamation starting at 12 hours PI. In the jejunum, loss of the epithelium led, via progressive shortening of the villi, to villous atrophy with temporary denudation of the mucosa at 16 to 18 hours PI. Polymorphonuclear cell infiltration occurred in intact pigs concomitantly with massive cell loss. In the upper duodenum and on the villi containing lymphoid tissue in the ileum, TGE virus replication was limited to a few cells and did not cause villous atrophy.The proliferative cells in the crypts of Lieberkiihn were refractory to TGE virus and this permitted healing of the viral injury to occur by rapid replacemen~ with newly regenerated cells which initially were flat to cuboidal. Differentiation of the cells was then observed and was followed by regrowth of the villi. Some of the regenerated cells were seen to become infected during this differentiation process. The majority of these cells, however, did not show any indication of intracellular viral activity. As villi grew longer, the number of infected cells gradually decreased and fluorescence was not detected after 7 days postinoculation.The events occurring in the isolated jejunal loops upon infection with TGE virus were, with some minor variations, similar to those in intact pigs. Newly 1 Submitted as Journal Paper No. 3966 of the Purdue University Agricultural Experiment Station. 2 Present address: Faculty of Veterinary Medicine, University of Ghent, Casinoplein 21, Ghent, Belgium. 21. 322 M. P]~NSAERT, E. O. HA]~LTERI~AN, and T. BURNSTEIN:produced virus was first detected in the lumen of the isolated loops at 6 hours. Virus yields reached maximum levels at 12 to 24 hours after infection and then progressively declined to be non-detectable at 15 to 18 days after inoculation.

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Studies on Transmissible Gastroenteritis in Pig in Japan

Cited by 13 publications

References 0 publications

Effects of ambient temperatures on clinical and immune responses of pigs infected with transmissible gastro-enteritis virus

Effects of ambient temperatures on clinical and immune responses of pigs infected with transmissible gastro-enteritis virus

Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus

Transmissible gastroenteritis of swine: Virus-intestinal cell interactions I. Immunofluorescence, histopathology and virus production in the small intestine through the course of infection

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