Studies on Trypsin Treatment of Red Cells with Special Reference to Differences between Trypsin Preparations

Abstract

Red cells were treated with 20 different trypsin preparations. When the red cells were used for detecting Rh-antibodies, the trypsins gave comparable results provided the solutions had the same trypsin activity as measured by Anson units. When testing for warm haemolysins, the effect on the red cells was dependent on the activity as measured by Anson units of the single trypsin preparation, but there were great differences between trypsin preparations which indicates some kind of qualitative differences between these latter. The findings also suggest that there are differences in specificity between warm haemolysins from different people.

Studies on the Agglutinin Accompanying the Warm Haemolysin Active against Trypsinized Red Cells

“…STI has previously [2] been shown to inhibit the ability of the trypsins to make red cells agglutinable by incomplete Rh-antibodies and lyseable by warm haemolysins.…”

“…The methods used in the present study are the same as those used in 3 previous papers [1][2][3]. The trypsin treatment was performed in principle according to the technique recommended by Pickles [4].…”

“…They are identified by their initials. Samples from several of them were also used in two previous papers [2,3].…”

“…The trypsin preparations are identified by the same numbers as in a previous paper [2] where further information was given.…”

“…(2) It cannot be demonstrated unless the serum is heated to 56 °C for 30 min even when its titre is several steps higher than that of the haemo lysin.…”