Studies on uronic acid materials

Anderson, D.M.W.; Karamalla, K. A.

doi:10.1016/s0008-6215(00)80334-1

Cited by 35 publications

(4 citation statements)

References 9 publications

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“…The highest cytotoxicity was observed in 250 µg/mL after 48 hours and IC 50 was 137.21 µg/mL. To the best of our knowl- Aqueous fraction of oleo-gum-resin is rich in different polysaccharides (8,17,18), such as 4-O-methylglucuronoarabino-galactan (19) 4-O-methylglucuronic acid (20). Polysaccharides from different origins have shown to have cytotoxic/anti-proliferative effects on various cell lines, including vascular smooth muscle cell (21), T-cell leukemia (22) and cervical cancer (23).…”

Section: Discussionmentioning

confidence: 99%

The Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

Jaafari‐Ashkavandi¹,

Hamedi²,

Assar³

et al. 2017

Int J Cancer Manag

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Background: Cytotoxic effects of Frankincense resin have been shown on some cancer cell lines. Due to its low side effects, this study was designed to evaluate the anticancer properties of water soluble elements of Frankincense oleo-gum-resin on human oral squamous cell carcinoma cell line. Methods:Oleo-gum-resin was macerated in ethanol. After filtration, the water soluble fraction of dried residue was extracted. KB cells were treated with 0, 62.5, 125, 250 and 500 µg/mL concentrations of obtained Frankincense aqueous fractions and with Doxorubicin as positive control. Frankincense induced cell cytotoxicity; apoptosis and proliferation were investigated using WST assay, Annexin V-FITC/PI, and Ki-67 staining, respectively. Data were analyzed using Kruskal-Wallis test by SPSS 17 software.Results: IC50 of 137.21 µg/mL was obtained from Frankincense aqueous fraction after 48 hours. The percentage of apoptotic cells was elevated in a time-and dose-dependent manner. There was no statistical difference in the Ki-67 expression of KB cells, using different concentration of Frankincense aqueous fraction after 24 and 48 hours (P = 0.083). Doxorubicin inhibited cells growth essentially through apoptosis. Conclusions:Frankincense aqueous fractions seem to suppress KB cell growth through the induction of apoptosis and necrosis rather than the inhibition of proliferation and hence might be a potential anticancer agent. Structural analysis and purification of potent components are suggested for determining more definitive results.

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Section: Discussionmentioning

confidence: 99%

The Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

Jaafari‐Ashkavandi¹,

Hamedi²,

Assar³

et al. 2017

Int J Cancer Manag

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“…The observation that 50% of the initial "C of these walls is extracted by chloroform after methylation suggests that the principal polymers of these two carbohydrates are represented in the derivatives of Table III. The Hakomori procedure can account for as much as 90% of the carbohydrates in dextrans (7) and a considerable proportion of the carbohydrates even in polyuronides (2) and in glycogen (5). Complete methylation of glucose or mannose polymers in germ tube walls cannot be assumed, however.…”

Section: Methodsmentioning

confidence: 99%

“…Further, there also are equivalent numbers of (1-*3) and (1-4) mannosidic linkages (Table II), but the number of cross linkages is higher than with the polymer from Puccinia and seem to involve mannose rather than glucose. Although identification is tentative because of lack of reference compounds, the branch appears to be (1)(2)(3)(4)(5)(6) (6,9).…”

Section: Methodsmentioning

confidence: 99%

“…Germ tube polymers appear more highly branched than uredospore wall polymers and only (1-* 3) mannose linkages were found. The main glucose linkages was also (1)(2)(3), but the nature of branch points was not established. Most known heteropolysaccharides with (1-*3) mannosidic linkages appear to exist in the a-anomeric form, yet neither a mannosidase, known to cleave (1-*2), (1-*3), and (1->6) mannosides, nor endo-/-mannanase, which may be active on /3(1-*3) linkages (10,16), released much mannose from germ tube wall.…”

Section: Methodsmentioning

confidence: 99%

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Cell Walls of Germinating Uredospores

Trocha

Daly²

1974

Plant Physiol.

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Polymeric carbohydrates in "C-labeled germ tube and uredospore walls of Uromyces phaseoli var. typica were studied by permethylation and by enzymatic hydrolysis. The native structure of the uredospore wall limited the effectiveness of both techniques with this wall, but evidence for two distinct polysaccharides was obtained. A linear (1-* 3) glucan, containing minor quantities of (1- There have been previous attempts to characterize polymers present in uredospores of rust fungi (6, 9). In these instances, carbohydrate polymers of intact ungerminated uredospores were first extracted by strong base and partially purified by precipitation as metal complexes. It is not clear how characteristic the reported structures are for the native wall. In the present research, structural analysis was attempted directly on the isolated walls.* MATERIALS AND METHODSThe preparation and isolation of "4C walls and some of the solvents used for chromatography have been described (14).Additional solvents were: C: benzene-ethanol-water-ammonia (200:47:15: 1); D: water-saturated 2-butanone; E: isopropanolacetic acid-water (54:8:18); F: ethyl acetate-pyridine-water (8:2:1); G: n-propyl alcohol-ethyl acetate-water (7:1:2). The following studies were carried out on wall fractions which had been treated with proteases, washed, and lyophilized (14).Methylation of Cell Walls. A modification of the Hakomori procedure (5) was employed for methylation of uniformly "C-labeled uredospore and germ tube walls. To form the methyl sulfinyl carbanion, 50% oil-coated sodium hydride was washed three times with hexane, dried in vacuo, and stirred in dry dimethyl sulfoxide at 60 C for 1 hr under N2. Lyophilized cell walls (50-500 mg) were placed in 5 to 10 ml of dimethyl sulfoxide, stirred under N2 for 1 hr at 60 C, and then added to an equal volume of methyl sulfinyl carbanion solution. A 25% excess of dry sodium hydride was used, based on the assumption of pure polysaccharide containing 5 mmoles of hydroxyl groups per 181 mg of wall preparation.The solution was stirred for 8 hr under N2 at room temperature. Methylation was carried out by dropwise addition of methyl iodide until the amount of methyl iodide was 1.5 times the assumed hydroxyl equivalents of the sample. The mixture was kept at room temperature for an additional 12 hr. Ice water (20 ml) was then added. Methylated polysaccharides were removed by extraction with chloroform (25 ml x 3). The combined extracts were washed with water (25 X 3) and evaporated to a syrup under vacuum. The

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Promising Gums from Sources other than Acacia senegal

Pinto

2000

Cell and Developmental Biology of Arabinogalactan-Proteins

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Studies on uronic acid materials

Cited by 35 publications

References 9 publications

The Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

The Effects of Frankincense on Oral Squamous Cell Carcinoma Cell Line

Cell Walls of Germinating Uredospores

Promising Gums from Sources other than Acacia senegal

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