Studies on Yeast Differentiation Using Organic Acid Metabolites Part 1. Development of Methodology Using High Performance Liquid Chromatography

Bell, D. J.; Blake, J. D.; Pražák, M.; Rowell, D.; Wilson, P. N.

doi:10.1002/j.2050-0416.1991.tb01072.x

Cited by 16 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chromatographic variables such as column temperature are known to affect the separation of co‐eluting peaks in organic acid metabolites and biomass hydrolysate samples . To improve the separation between DMC and EC, the effect of the change in column temperature on the resolution of these carbonates was studied at four temperatures (55, 45, 35 and 25°C) while keeping the remaining parameters the same (Fig.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Method development for separating organic carbonates by ion‐moderated high‐performance liquid chromatography

Bhalkikar

Marin

Cheung

2016

J of Separation Science

View full text Add to dashboard Cite

An ion-moderated partition high-performance liquid chromatography method was developed for the separation and identification of common organic carbonates. The separation of organic carbonates was achieved on an ion exclusion column with an exchangeable hydrogen ion. An isocratic, aqueous mobile phase was used for elution and detection was performed with a refractive index detector. The developed method was validated for specificity, linearity, limits of detection and quantification, precision and accuracy. All calibration curves showed excellent linear regression (R > 0.9990) within the testing range. The limits of detection were 3.8-30.8 ppm for the analyzed carbonates. Improvements in the peak resolution of the chromatograms were achieved by decreasing the column temperature. Addition of the organic modifier, acetonitrile, to the eluent was found to have insignificant effects on the peak resolution. The developed method was demonstrated for analyzing organic carbonate components in the electrolyte system of a commercial lithium ion battery.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Chromatographic variables such as column temperature are known to affect the separation of co-eluting peaks in organic acid metabolites [29] and biomass hydrolysate samples [30].…”

Section: Effect Of the Column Temperature On The Elution Of Dimethyl mentioning

confidence: 99%

Method development for separating organic carbonates by ion‐moderated high‐performance liquid chromatography

Bhalkikar

Marin

Cheung

2016

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…VFAs were separated on an Aminex HPX-87H organic acid column (Bio-Rad, Hercules, CA, USA) with a mobile phase containing 0.2% phosphoric acid and 5% acetonitrile in MilliQ water, at a flow rate of 0.9 mL min −1 . The column was maintained at 85 °C and the acids were quantified with the photodiode detector at set at 210 nm (Bell et al 1991 ). Orotic acid, uracil, and thymine were separated on the same column but at room temperature, with a flow of 0.5 mL min −1 , and with 0.008 N sulfuric acid as mobile phase.…”

Section: Methodsmentioning

confidence: 99%

Characterization of volatile fatty-acid utilization in Escherichia coli aiming for robust valorisation of food residues

2020

View full text Add to dashboard Cite

Valorisation of food residues would greatly benefit from development of robust processes that create added value compared to current feed- and biogas applications. Recent advances in membrane-bioreactor-based open mixed microbial cultures, enable robust conversion of fluctuating streams of food residues to a mixture of volatile fatty acids (VFAs). In this study, such a mixed stream of VFAs was investigated as a substrate for Escherichia coli, a well-studied organism suitable for application in further conversion of the acids into compounds of higher value, and/or that are easier to separate from the aqueous medium. E. coli was cultured in batch on a VFA-rich anaerobic digest of food residues, tolerating up to 40 mM of total VFAs without any reduction in growth rate. In carbon-limited chemostats of E. coli W3110 ΔFadR on a simulated VFA mixture, the straight-chain VFAs (C2-C6) in the mixture were readily consumed simultaneously. At a dilution rate of 0.1 h−1, mainly acetic-, propionic- and caproic acid were consumed, while consumption of all the provided acids were observed at 0.05 h−1. Interestingly, also the branched isovaleric acid was consumed through a hitherto unknown mechanism. In total, up to 80% of the carbon from the supplied VFAs was consumed by the cells, and approximately 2.7% was excreted as nucleotide precursors in the medium. These results suggest that VFAs derived from food residues are a promising substrate for E. coli.

show abstract

“…VFAs were separated on an Aminex HPX-87H organic acid column (Bio-Rad, Hercules, CA, USA) with a mobile phase containing 0.2% phosphoric acid and 5% acetonitrile in MilliQ water, at a ow rate of 0.9 mL min − 1 . The column was maintained at 85 °C and the acids were quanti ed with the photodiode detector at set at 210 nm (Bell et al 1991). Orotic acid, uracil, and thymine were separated on the same column but at room temperature, with a ow of 0.5 ml min − 1 , and with 0.008 N sulfuric acid as mobile phase.…”

Section: Analysesmentioning

confidence: 99%

Characterization of volatile fatty-acid utilization in Escherichia coli aiming for robust valorisation of food residues

Sjöberg

Gustavsson

Maris

2020

Preprint

View full text Add to dashboard Cite

Valorisation of food residues would greatly benefit from development of robust processes that create added value compared to current feed- and biogas applications. Recent advances in membrane-bioreactor-based open mixed microbial cultures, enable robust conversion of fluctuating streams of food residues to a mixture of volatile fatty acids (VFAs). In this study, such a mixed stream of VFAs was investigated as a substrate for Escherichia coli, a well-studied organism suitable for application in further conversion of the acids into compounds of higher value, and/or that are easier to separate from the aqueous medium. E. coli was cultured in batch on a VFA-rich anaerobic digest of food residues, tolerating up to 40 mM of total VFAs without any reduction in growth rate. In carbon-limited chemostats of E. coli W3110 ΔFadR on a simulated VFA mixture, the straight-chain VFAs (C2-C6) in the mixture were readily consumed simultaneously. At a dilution rate of 0.1 h− 1, mainly acetic-, propionic- and caproic acid were consumed, while consumption of all the provided acids were observed at 0.05 h− 1. Interestingly, also the branched isovaleric acid was consumed through a hitherto unknown mechanism. In total, up to 80% of the carbon from the supplied VFAs was consumed by the cells, and approximately 2.7% was excreted as nucleotide precursors in the medium. These results suggest that VFAs derived from food residues are a promising substrate for E. coli.

show abstract

Studies on Yeast Differentiation Using Organic Acid Metabolites Part 1. Development of Methodology Using High Performance Liquid Chromatography

Cited by 16 publications

References 9 publications

Method development for separating organic carbonates by ion‐moderated high‐performance liquid chromatography

Method development for separating organic carbonates by ion‐moderated high‐performance liquid chromatography

Characterization of volatile fatty-acid utilization in Escherichia coli aiming for robust valorisation of food residues

Characterization of volatile fatty-acid utilization in Escherichia coli aiming for robust valorisation of food residues

Contact Info

Product

Resources

About