Studies on yeast metabolism. 7. Yeast carbohydrate fractions. Separation from nucleic acid, analysis, and behaviour during anaerobic fermentation

Trevelyan, W. E.; Harrison, Joseph S.

doi:10.1042/bj0630023

Cited by 214 publications

(81 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Yeast samples (40 mg dry wt) were washed three times with ice-cold water and stored at -17 "C prior to analysis. Carbohydrates were fractionated according to the method of Trevelyan & Harrison (1956) as reported by Stewart (1975), and fractions were analysed in triplicate using the anthrone procedure (Stewart, 1975). Glucose and dry weight were determined as described previously (Quain & Haslam, 1979).…”

Section: Methodsmentioning

confidence: 99%

Changes in Glucose 6-Phosphate and Storage Carbohydrates during Catabolite Derepression in Saccharomyces cerevisiae

Quain¹,

Haslam²

1979

Journal of General Microbiology

View full text Add to dashboard Cite

Changes in the cellular content of glucose 6-phosphate and storage carbohydrates during cataboli te derepression were measured during the diauxic growth of Saccharornyces cerevisiae on glucose (1 %, w/v) medium. After the exhaustion of glucose there were transient increases in glucose 6-phosphate, alkali-soluble glycogen and trehalose, reflecting enhanced gluconeogenesis, followed by an increase in acid-soluble glycogen. During the subsequent adaptation to growth on ethanol the amounts of glucose 6-phosphate, trehalose and glycogen returned to basal levels. Thus storage carbohydrates, of which glycogen is quantitatively the most important, appear to act as the energy source for catabolite derepression.

show abstract

Section: Methodsmentioning

confidence: 99%

Changes in Glucose 6-Phosphate and Storage Carbohydrates during Catabolite Derepression in Saccharomyces cerevisiae

Quain¹,

Haslam²

1979

Journal of General Microbiology

View full text Add to dashboard Cite

show abstract

“…Trehalose was extracted from yeast (Lillie and Pringle, 1980) and determined by the anthrone method (Trevelyan and Harrison, 1956). The results were confirmed by HPLC analysis with an HP 1081B instrument using a CaZ+ cationexchange column at 85"C, with water as eluent (flow rate 0.5 ml min-') and trehalose dihydrate (Alltech) as standard.…”

Section: Enzyme and Protein Assays And Trehalose Determinationmentioning

confidence: 99%

Cloning of two related genes encoding the 56‐kDa and 123‐kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae

Vuorio¹,

Kalkkinen

Londesborough³

1993

European Journal of Biochemistry

143

127

View full text Add to dashboard Cite

Preparations of intact trehalose synthase contain three polypeptides with molecular masses of 56, 102 and 123 kDa. We have cloned the genes TSSl and TSLl coding for the 56-and 123-kDa subunits, respectively. These genes are located on chromosomes I1 (TSSI) and XI11 (TSLI). The TSSl gene was found to be identical with ClFl, a gene required for normal growth on glucose. The product of the entire TSSl gene exibits 37% identity with a 502-amino-acid stretch from the middle of the TSLl product. Disruption of the TSSl gene in yeast eliminates both trehalose 6-phosphate synthase (Tre6P synthase) and trehalose 6-phosphate phosphatase (Tre6Pase) activities, and reintroduction of this gene restores these activities. Transformation of Escherichia coli with TSSl increases its Tre6P synthase activity. Specific proteolytic degradation of the 123-kDa polypeptide from the N-terminus greatly influences the Tre6P synthase activity, decreasing its inhibition by phosphate and activatability by fructose 6-phosphate but has little effect on the Tre6Pase activity. These results suggest that this N-terminal part confers regulatory properties upon the Tre6P synthase activity.Trehalose, a non-reducing disaccharide of glucose, occurs in Saccharomyces cerevisiae during periods of reduced growth, such as during starvation of cells for nitrogen, phosphate or sulfur, as well as during the stationary phase after growth on glucose (Lillie and Pringle, 1980). It is also accumulated in cells exposed to extremes of temperature, dehydration or hazardous chemicals (Attfield, 1987 ;Hottiger et al., 1987;Hino et al., 1990). Experiments using mutants producing varying amounts of trehalose and the increases in intracellular trehalose concentration caused by heat-shock treatment, have shown that heat tolerance, as well as desiccation tolerance, are closely related to the trehalose content of the yeast cell (Hottiger et al., 1987).The formation of trehalose is catalysed by the sequential action of trehalose-6-phosphate synthase (Tre6P synthase) and trehalose-6-phosphate phosphatase (Tre6Pase) activities. These activities co-elute through several chromatographic procedures, suggesting that they are part of a single bifunctional protein (Vandercammen et al., 1989 ;Londesborough and Vuorio, 1991). A protein complex, called trehalose synthase, has been purified in a specifically proteolysed form (Londesborough and Vuorio, 1991) and an apparently intact form (Vuorio et al

show abstract

“…Trehalose was extracted from 60 mg yeast (wet basis) using cold 0.5M trichloroacetic acid according to Trevelyan and Harrison (23,24) and then determined through the anthrone reaction (3). The colored product was detected at wavelength 620 nm and the results were presented as g of trehalose/ 100g of dry matter.…”

Section: Methodsmentioning

confidence: 99%

Mobilization of endogenous glycogen and trehalose of industrial yeasts

Paulillo¹,

Yokoya²,

Basso³

2003

Braz. J. Microbiol.

View full text Add to dashboard Cite

The fermentation of yeast reserve carbohydrates, glycogen and trehalose is a procedure to increase protein level of yeast cells and improve ethanol production. This work studied on the degradation kinetics of glycogen and trehalose carried out with two industrial strains of Saccharomyces cerevisiae (PE-2 and SA-1) and the effect of different temperatures (38º, 40º, 42º and 44ºC) on degradation rate. Endogenous fermentation was carried out in a yeast suspension at 20% (w/v) based on wet weight, suspended in fermented media with 3.0 to 4.5 % (v/v) of ethanol. The degradation of the carbohydrate reserves at 40ºC followed first-order kinetics, showing that its rate is mainly dependent on the carbohydrate concentration in the cell. The degradation rate (k) ranged from 0.0387 to 0.0746 h -1 . Analyzing other parameters at 40ºC, it was observed that viability and dry and wet yeast biomass were reduced while cell protein, ethanol, glycerol and nitrogen in the medium increased. Glycogen and trehalose degradation at different temperatures (38º, 40º, 42º e 44ºC) showed that at 38ºC the degradation rate was slow and from 42ºC on the degradation of glycogen stopped after few hours of incubation. Thus, from a practical point of view, the best incubation temperature is around 40ºC. The application of the Arrhenius equation showed that activation energy from 40º to 42ºC was 165.90 and 107.94 kcal. o K -1 .mol -1 for trehalose and glycogen respectively for PE-2 strain, and 190.64 and 149.87 kcal. o K -1 .mol -1 respectively for SA-1 strain.

show abstract

Studies on yeast metabolism. 7. Yeast carbohydrate fractions. Separation from nucleic acid, analysis, and behaviour during anaerobic fermentation

Cited by 214 publications

References 15 publications

Changes in Glucose 6-Phosphate and Storage Carbohydrates during Catabolite Derepression in Saccharomyces cerevisiae

Changes in Glucose 6-Phosphate and Storage Carbohydrates during Catabolite Derepression in Saccharomyces cerevisiae

Cloning of two related genes encoding the 56‐kDa and 123‐kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae

Mobilization of endogenous glycogen and trehalose of industrial yeasts

Contact Info

Product

Resources

About