Studies onAzotobacter species in soil

Brown, Michael; Burlingham, Susan K.; Jackson, R. M.

doi:10.1007/bf01377670

Cited by 80 publications

(7 citation statements)

References 5 publications

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“…A total of 20 g of soil was mixed in a flask with 100 mL of distilled water and shaken for 12 h. Then, the soil extract was filtered and used to determine organic acid and phenolic compounds [ 33 ]. The percentage of CaCO 3 was estimated according to Brown et al [ 34 ]. The contents of carbon (C) and nitrogen (N) were measured by CN element analyzer (NC-2100, Carlo Erba Instruments, Milan, Italy).…”

Section: Methodsmentioning

confidence: 99%

“…Disc diffusion method [ 33 ] was used for assaying the potential antimicrobial activity of tissue ethanolic extracts against Escherichia coli and Streptococcus sp using a suspension (100 μL containing 1 × 10 8 CFU/mL) of bacteria spread on Muller Hinton agar (SDA) [ 34 ]. Negative controls were prepared using the same solvent employed to dissolve extracts.…”

Section: Methodsmentioning

confidence: 99%

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Actinomycetes Enrich Soil Rhizosphere and Improve Seed Quality as well as Productivity of Legumes by Boosting Nitrogen Availability and Metabolism

et al. 2020

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The use of actinomycetes for improving soil fertility and plant production is an attractive strategy for developing sustainable agricultural systems due to their effectiveness, eco-friendliness, and low production cost. Out of 17 species isolated from the soil rhizosphere of legume crops, 4 bioactive isolates were selected and their impact on 5 legumes: soybean, kidney bean, chickpea, lentil, and pea were evaluated. According to the morphological and molecular identification, these isolates belong to the genus Streptomyces. Here, we showed that these isolates increased soil nutrients and organic matter content and improved soil microbial populations. At the plant level, soil enrichment with actinomycetes increased photosynthetic reactions and eventually increased legume yield. Actinomycetes also increased nitrogen availability in soil and legume tissue and seeds, which induced the activity of key nitrogen metabolizing enzymes, e.g., glutamine synthetase, glutamate synthase, and nitrate reductase. In addition to increased nitrogen-containing amino acids levels, we also report high sugar, organic acids, and fatty acids as well as antioxidant phenolics, mineral, and vitamins levels in actinomycete treated legume seeds, which in turn improved their seed quality. Overall, this study shed the light on the impact of actinomycetes on enhancing the quality and productivity of legume crops by boosting the bioactive primary and secondary metabolites. Moreover, our findings emphasize the positive role of actinomycetes in improving the soil by enriching its microbial population. Therefore, our data reinforce the usage of actinomycetes as biofertilizers to provide sustainable food production and achieve biosafety.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Actinomycetes Enrich Soil Rhizosphere and Improve Seed Quality as well as Productivity of Legumes by Boosting Nitrogen Availability and Metabolism

et al. 2020

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“…Bacteria inocula were grown in 250-mL Erlenmeyer flasks with 150 mL of medium for Azospirillum brasilense (Okon et al 1977) or Azotobacter chroococcum (Brown et al 1962). Strains were incubated at 32 ± 1°C on an orbital Sontec TM shaker (140 rpm); Azospirillum brasilense for 72 h and Azotobacter chroococcum for 6 days.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

Anatomy and morphology of photinia (Photinia × fraseri Dress) in vitro plants inoculated with rhizobacteria

Larraburu

Apóstolo

Llorente

2010

Trees

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Photinia 9 fraseri Dress (photinia) is a woody plant with high ornamental value. The anatomy and morphology of micropropagated photinia inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense and Azotobacter chroococcum, in combination with pulses of 49.2 lM indole-3-butyric acid during rhizogenesis, were characterized using light and electron microscopy. Leaves of inoculated in vitro plants showed better development than those subjected to auxin control only. All inoculated treatments, independent of the bacterial strain used, had leaves with two layers of palisade parenchyma, a thick cuticle and linear unicellular trichomes. There was no proliferation of undifferentiated tissue in any treatment and the plants showed shoot-root vascular connections. Ex vitro leaves and in vitro plants inoculated with Azospirillum brasilense Cd and Azotobacter chroococcum 42 had large stomata with elliptic aperture radially surrounded by small stomata on the abaxial foliar surface. In addition, plants of these treatments had a large root hair zone over the root surface. Bacteria were only observed on surfaces of root hairs. The results suggest that the structural changes induced by bacterial inoculation of photinia in vitro plants could lead to better adaptation to ex vitro conditions after transplanting.

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“…Strain CBS 11561 was isolated as a separate colony and strain CBS 11562 T grew in a mixed culture with Trichosporon porosum . The latter culture was purified on modified Browns’ nitrogen deficient media (Brown et al , 1962) supplemented with imidazole (according to LaRue & Spencer, 1967) and cycloheximide (according to Danielson & Jurgensen, 1973). Cultures were maintained on MYP medium (Sampaio et al , 2003).…”

Section: Methodsmentioning

confidence: 99%

Leucosporidium drummii sp. nov., a member of the Microbotryomycetes isolated from soil

Yurkov

Schäfer

Begerow

2012

International Journal of Systematic and Evolutionary Microbiology

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Two strains of a novel teleomorphic basidiomycete were isolated from grassland soil. Standard phenotypic tests and phylogenetic analyses of 26S rRNA gene (D1/D2 domains) and ITS region sequences showed that the species belongs to the core group of the genus Leucosporidium. A novel species, Leucosporidium drummii sp. nov., is proposed to accommodate the two strains, with SEG-3-2-AY220T ( = CBS 11562T = MUCL 52878T) as the type strain. In addition, phylogenetic analysis revealed great genetic variability in the Leucosporidium scottii complex.

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Studies onAzotobacter species in soil

Cited by 80 publications

References 5 publications

Actinomycetes Enrich Soil Rhizosphere and Improve Seed Quality as well as Productivity of Legumes by Boosting Nitrogen Availability and Metabolism

Actinomycetes Enrich Soil Rhizosphere and Improve Seed Quality as well as Productivity of Legumes by Boosting Nitrogen Availability and Metabolism

Anatomy and morphology of photinia (Photinia × fraseri Dress) in vitro plants inoculated with rhizobacteria

Leucosporidium drummii sp. nov., a member of the Microbotryomycetes isolated from soil

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