Ethylene had been shown to be an important senescence (1) and abscission (1,11,14) promoting agent. Both ethylene gas (5, 12) and ethylene-releasing compounds (13,16,21) have been used in abscission studies. As these compounds have been generally applied exogenously, the metabolic response depended on penetration and, with the ethylene-releasing compounds, on a nonmetabolic degradation (6, 15). Until recently, known metabolic precursors of ethylene did not significantly increase ethylene evolution. The recent work of Adams and Yang (3) described the role of ACC2 in the pathway of ethylene biosynthesis. This last step precursor of ethylene, unaffected by AVG, is readily metabolized (9, 23) to ethylene and application of ACC to various plant tissues results in a rapid increase in ethylene evolution (10).Endogenous ethylene evolution, as well as that induced by an ethylene-releasing compound (ET), in relation to abscission of various olive organs, has been established (19). In previous studies (17, 18) we introduced an in vitro system in which ET was fed to the shoots via the cut base and moved in the transpiration stream. In these studies we reported abscission induced by ET to be concentration and application time-dependent. Under the conditions reported, leaf abscission was induced, whereas inflorescences ' On sabbatical leave