Studies With Inagglutinable Erythrocyte Counts. Iii. Kinetics of Erythrocyte Destruction in Human Beings 1

Abstract

Many investigators

Studies With Inagglutinable Erythrocyte Counts. V. Spontaneous and X-Ray-Induced Hemolysis in Malignancy 12

“…The Our methods of enumerating erythrocyte and inagglutinable cells have been reported and discussed in detail previously (12,13).…”

Studies With Inagglutinable Erythrocyte Counts. Vi. Accelerated Destruction of Normal Adult Erythrocytes in Pernicious Anemia; Contribution of Hemolysis to the Oligocythemia 12

“…After transfusion the donor's cell counts were estimated in the recipient's circulation by a modification of the Ashby differential agglutination technique previously described and evaluated by us (7,8 …”

The Use of DFP32 as a Red Cell Tag with and without Simultaneous Tagging with Chromium51 in Certain Animals in the Presence or Absence of Random Destruction

“…On the other hand the Cr st data presented a different kind of difficulty. Because of the normal age distribution of cells and absence of random destruction, it was expected that the data would fit the equation of Sheets et al (1). However, attempts to fit the data by the method of Deming (2) the elution rate, k, was not constant but dropped during the first week or so from a high initial value to a much lower but approximately constant rate.…”

“…in which red cell destruction can be attributed entirely to senescence, difficulties in interpretation resulting from elution are evident. Thus when radiochromium is used, decrease in radioactivity in the blood is no longer linear, and the disappearance curve is described by the equation originally derived by Sheets et al (1) y = yo(l --t/T)e -k~ (i) in which y is the radioactivity count on day t corrected for decay, y0 being the count for day zero, the day of transfusion. The average potential life span of the erythrocyte is T days, and the fraction of chromium lost daily by elution is k. Data can be fitted satisfactorily and without bias to this equation by the method of Deming (2), but the procedure is very laborious, particularly since it is a method of successive approximations, and may require five or more repetitions to reach stable values.…”