Studies with Polysomes Obtained from Rabbits During the Immune Response to Ragweed Antigen

Uptake of Carbohydrate by Polyribosomes During the Immune Response to Ragweed Antigen

“…In previous studies [22], the authors reported the effect of secondary immunization on the incorporation of labeled amino acids into rabbit It should be noted that in all the gradients depicted in figures 1 and 2, a peak of radioactivity is discernible in the region 45% of the distance from the meniscus and, to a lesser extent, at 55% of the distance. Our previous studies have shown that at 45% of the distance from the menis cus polysomes having 6-7 ribosomes predominate and at 55% poly somes with 13-16 ribosomes.…”

“…New Zealand white rabbits (2-2.5 kg) were immu nized by a method described previously [22] with whole aqueous extract of dwarf ragweed pollen. In some cases, a solution of the purified dwarf ragweed antigen, Pool C [3] was used for immunization (1 mg/ml 0.85°/o NaCl).…”

“…From the recorder tracings, the average value of A254 n|n (1 cm light path) of each fraction was cal culated for the purpose of plotting the results. For measurement of radioactivity, the fractions were diluted to 3.0 ml with MSB solution and 1.5 ml of diluted sam ple was admixed with 15 ml of scintillation fluid containing triton X-100 as de scribed previously [22]. Internal standards were used to convert all backgroundcorrected counts to disintegrations/min.…”

“…The precipitate was then dissolved in 1.0 ml of hydroxide of Hyamine (Packard Instrument Co., Downers Grove, 111.) at 60 °C and admixed with 15 ml of scintillation fluid as described previously [22], Chemicals. D-Glucosamine hydrochloride and N-acetyl-D-glucosamine were ob tained from Calbiochem, Los Angeles, Calif.; uridine diphospho-A/-acetylglucosamine and pancreatic ribonuclease were obtained from Sigma Chemical Co., St. Louis, Mo.…”