We have studied the effect of the biodegradable polymer TPA (Thermal Polyaspartate Anion) on the acidity of diesel fuel and its blends with biodiesel. We determine the effectiveness of the polymer based on the acid number of the polymer/fuel composition. The results show that the acid number is significantly reduced when the polymer is added, i.e., the quality of the fuel is improved.Diesel fuel is a mixture of various n-paraffins and isoparaffins, naphthenes, olefins, and aromatic hydrocarbons, each with their own physical and chemical properties [1]. Diesel fuel must satisfy a wide range of engine types, differing operating conditions and duty cycles, as well as variations in fuel system technology, engine temperatures, and fuel system pressures. Furthermore, the characteristics of each grade of diesel fuel must also be suitable for a variety of climates. To some extent, quality standards represent a certain compromise so that all the fuel performance requirements may be satisfied. By controlling the properties of diesel fuel, it is possible to use one grade of fuel in millions of compression-ignition engines [2].In addition, biodiesel has become a fundamentally important area of focus for work in both the scientific and production sectors, since this alternative energy source can reduce the use of conventional fuels [3]. It is particularly important to note that biodiesel (initially biodiesel/diesel blends and ultimately pure biodiesel) is suitable from both an energy standpoint and in terms of environmental friendliness.Evaluation of the fuel quality (conventional fuel, nonconventional fuel, or blends of both) is aimed at identifying the degree of environmental impact of the fuel, the engine noise level, and the maximum power of the engine when running on this fuel. Quality analysis of a fuel involves determining its characteristics, the permissible values of which have been established by the standards. Analysis methods for fuels have been established by ASTM and IP (Institute of Petroleum) standards.One of the most important characteristics of biodiesel and biodiesel blends with conventional fuel is the acid number, which is an indicator of the degree of oxidation of the fuel and affects its combustion. Determination