Study of Acinetobacter Species with Special Reference to Species Differentiation and Metallo-Beta Lactamase Production in a Rural Tertiary Care Hospital

Anagha, Kulkarni Vishal Kinikar; Sachin, Bhalerao Deepika Deorukhkar; Shahriar, Roushani

doi:10.20546/ijcmas.2017.609.132

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…The highest number of Acinetobacter spp. were isolated from pus specimens (60.24%) followed by sputum specimens (13.25%) and urine specimens (8.43%) which is similar with other studies conducted by Kulkarni et al (2017).…”

Section: Discussionsupporting

confidence: 89%

Biofilm Production and Antibiotic Resistance in Clinical Isolates of Acinetobacter Species

Bhandari,

Chhunju,

Nayaju

et al. 2023

TU J. Microbiol.

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Objective: To study the occurrence of the biofilm producing Acinetobacter spp. from different clinical specimens and to assess the antibiotic susceptibility pattern of Acinetobacter species. Methods: This study was conducted at B&B Hospital Pvt. Ltd., Lalitpur, Nepal from February to September 2018. Various specimens including pus, sputum, urine, catheter tips, body fluids (bile, peritoneal fluid, CSF), suction tube, and blood were collected from the patients (n=5141) visiting B&B hospital. The bacterial isolates were subjected to antibiotic susceptibility testing and Acinetobacter spp. isolates were subjected to biofilm detection by microtiter plate method. Results: Out of 5141 specimens, 1179 (23%) were culture positive. Escherichia coli (40.8%) was found to be the predominant organism. A total of 83 isolates of Acinetobacter spp. were isolated among which 76(91.57%) were biofilm producers. Biofilm producing isolates of Acinetobacter spp. were found more resistant to the tested antibiotics than non- biofilm producing Acinetobacter spp. Conclusion: Most Acinetobacter spp. was capable of producing the biofilm. The biofilm producers were more resistant to the antibiotics under study which help to increase the resistivity nature of the bacteria. All of the isolates susceptible to colistin showed that the appropriate therapeutic option for infection caused by biofilm forming Acinetobacter spp.

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Section: Discussionsupporting

confidence: 89%

Biofilm Production and Antibiotic Resistance in Clinical Isolates of Acinetobacter Species

Bhandari,

Chhunju,

Nayaju

et al. 2023

TU J. Microbiol.

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Phenotypic characterization of Acinetobacter species and their resistance pattern in a tertiary care hospital

Bhatawadekar¹,

Ravan

Modak³

2022

IJMMTD

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is an emerging opportunistic pathogen and increasingly implicated in hospital acquired infection especially in intensive care unit. The infection caused by spp. is difficult to control due to multi drug resistance which limits its therapeutic options. This study was undertaken to isolate from various clinical samples and to speciate isolates. Efforts were made to study the antimicrobial susceptibility pattern of species and to detect metallo beta lactamase producing strains. Clinical samples blood, urine, sputum, pus, body fluids and ETT sample received in microbiology laboratory were processed according to the standard laboratory procedure. Identification and antimicrobial susceptibility testing was done by VITEK-2 automated method. All the isolates that showed resistance to Imipenem were tested for Metallo – Beta – Lactamase (MBL) production by phenotypic test, Imipenem-EDTA-combined disc test method. Out of total 100 spp isolated from clinical samples, 80 were isolated from critical care units. baumannii was common spp 88 (88%), followed by A.complex 10 (10%). Isolation rate of species was highest from blood (27%) specimens followed by ETT (19%). 44% strains of were isolated from respiratory samples. 84(84%) spp, were carbapenem resistant. Out of 84 carbapenems resistant strains 48 (57%) were MBL positive strains by combined disc test. Emergence of MBLs producing strains of is alarming and reflects excessive use of carbapenems. Therefore early detection and prompt infection control measures is important to prevent further spread of MBLs to other Gram negative bacilli.

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Study of Acinetobacter Species with Special Reference to Species Differentiation and Metallo-Beta Lactamase Production in a Rural Tertiary Care Hospital

Cited by 2 publications

References 20 publications

Biofilm Production and Antibiotic Resistance in Clinical Isolates of Acinetobacter Species

Biofilm Production and Antibiotic Resistance in Clinical Isolates of Acinetobacter Species

Phenotypic characterization of Acinetobacter species and their resistance pattern in a tertiary care hospital

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