Study of Adipose Tissue-Derived Mesenchymal Stem Cells Transplantation for Rats with Dilated Cardiomyopathy

Li, Liang; Xia, Yunlong

doi:10.5761/atcs.oa.13-00104

Cited by 14 publications

(8 citation statements)

References 17 publications

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“…Caspase 3 Ab to detect apoptotic cells ( 20 ) and CD44 immunostaining for detecting endogenous and exogenous SCs ( 21 ) were used. Ready to use rabbit polyclonal caspase 3 (CPP32) Ab-4 IgG (RB-1197-R7, Thermo Fisher Scientific; Fremont, USA) and ready to use rabbit polyclonal CD44 Ab; IgG (IW-PA1021, IHC World) were used.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic Effect of Adipose Derived Stem Cells versus Atorvastatin on Amiodarone Induced Lung Injury in Male Rat

Aboul-Fotouh

Zickri

Gabr

et al. 2015

IJSC

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Background and ObjectivesAmiodarone (AM), a class 3 antiarrhythmic drug, has been associated with variety of adverse effects, the most serious of which is pulmonary toxicity. Ator (A) is a statin, known for their immunomodulatory and anti-inflammatory activities. Recent studies provide evidence of potential therapeutic effect of statins on lung injury. Adipose derived stem cells (ADSCs) have shown great promise in the repair of various tissues. The present study aimed at investigating and comparing the possible therapeutic effect of A and ADSCs on AM induced lung injury in albino rats.Methods and Results34 adult male albino rats were divided into 5 groups: control group (Gp I), A group (Gp II) received 10 mg/kg of A orally 6 days (d)/week (w) for 4 weeks (ws), AM group (Gp III) received 30 mg/kg of AM orally 6 d/w for 4 ws, AM&A group (Gp IV) received AM for 4ws then A for other 4 ws and AM&SCs group (Gp V) received AM for 4 ws then injected with 0.5 ml ADSCs on 2 successive days intravenously (IV). Histological, histochemical, immunohistochemical and morphometric studies were performed. Group III displayed bronchiolitis obliterans, thickened interalveolar septa (IAS) and thickened vascular wall which were proven morphometrically. Increased area% of collagen fibers and apoptotic changes were recorded. All findings regressed on A administration and ADSCs therapy.ConclusionAtor proved a definite ameliorating effect on the degenerative, inflammatory, apoptotic and fibrotic changes induced by AM. ADSCs administration denoted more remarkable therapeutic effect compared to A.

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Section: Methodsmentioning

confidence: 99%

Therapeutic Effect of Adipose Derived Stem Cells versus Atorvastatin on Amiodarone Induced Lung Injury in Male Rat

Aboul-Fotouh

Zickri

Gabr

et al. 2015

IJSC

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“…SVF is an attractive therapeutic method given that the harvesting process is safe and the cells are readily available in usually large quantities. Transplantation of SVF or ADSCs in animal models of myocardial infarction [ 20 , 21 ] and dilated cardiomyopathy [ 22 ] significantly improves left ventricular cardiac function and decreases mortality after cell transplantation [ 15 , 23 – 26 ]. The benefits of ADSCs are postulated to come from their influence on neovascularization of the ischemic tissue and their protection of resident cells [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of the intramyocardial implantation of stromal vascular fraction in patients with chronic ischemic cardiomyopathy

Comella

Parcero

Bansal³

et al. 2016

J Transl Med

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BackgroundStromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue. The SVF contains a mixture of cells including ADSCs and growth factors and has been depleted of the adipocyte (fat cell) population. We evaluated the safety and efficacy of administering SVF intra-myocardially into patients with chronic ischemic cardiomyopathy.MethodsA total of 28 patients underwent a local tumescent liposuction procedure to remove approximately 60 ml of fat tissue. The fat was separated to isolate the SVF and the cells were delivered into the akinetic myocardial scar region using a transendocardial delivery system (MyoCath®) in patients who had experienced a previous myocardial infarct. The subjects were then monitored for adverse events, ejection fraction via echocardiogram and six-minute walk test (6MWT) over a period of 6 months.ResultsThe average EF was 29 % at baseline and significantly increased to 35 % at both 3 and 6 months. Patients walked an average of 349 m at baseline and demonstrated a statistically significant improvement at 3 and 6 months’ post treatment of more than 80 m.ConclusionsOverall, patients were pleased with the treatment results. More importantly, the procedure demonstrated a strong safety profile with no severe adverse events or complications linked to the therapy.Trial registration NCT01502514Name of registry: http://www.clinicaltrials.govURL: https://www.clinicaltrials.gov/ct2/show/NCT01502514?term=adipose+cells+heart&rank=4Date of registration: December 27, 2011Date of enrollment: January 2012

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“…Our data confirm that 5-azacytidine is a controversial cardiac differentiation agent (27,44,55) and that it has not yet been proven to induce terminally differentiated cells (52). Adipose tissue-derived MSCs are able to differentiate into a cardiac phenotype even without 5-azacytidine induction (25). Besides, it is noteworthy to highlight that 5-azacytidine is considered a toxic chemical agent (although not toxic in our conditions as indicated by the MTS assay showing comparable proliferative rate to FBS; data not shown); thus, it cannot be considered an eligible candidate from a clinical standpoint.…”

Section: Discussionmentioning

confidence: 99%

Cardiosphere Conditioned Media Influence the Plasticity of Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells

Siciliano

Chimenti

Ibrahim³

et al. 2015

Cell Transplant

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Nowadays, cardiac regenerative medicine is facing many limitations because of the complexity to find the most suitable stem cell source and to understand the regenerative mechanisms involved. Mesenchymal stem cells (MSCs) have shown great regenerative potential due to their intrinsic properties and ability to restore cardiac functionality, directly by transdifferentiation and indirectly by paracrine effects. Yet, how MSCs could respond to definite cardiac-committing microenvironments, such as that created by resident cardiac progenitor cells in the form of cardiospheres (CSs), has never been addressed. Recently, a putative MSC pool has been described in the mediastinal fat (hmADMSCs), but both its biology and function remain hitherto unexplored. Accordingly, we investigated the potential of hmADMSCs to be committed toward a cardiovascular lineage after preconditioning with CS-conditioned media (CCM). Results indicated that CCM affects cell proliferation. Gene expression levels of multiple cardiovascular and stemness markers (MHC, KDR, Nkx2.5, Thy-1, c-kit, SMA) are significantly modulated, and the percentage of hmADMSCs preconditioned with CCM and positive for Nkx2.5, MHC, and KDR is significantly higher relative to FBS and explant-derived cell conditioned media (EDCM, the unselected stage before CS formation). Growth factor-specific and survival signaling pathways (i.e., Erk1/2, Akt, p38, mTOR, p53) present in CCM are all equally regulated. Nonetheless, earlier BAD phosphorylation (Ser112) occurs associated with the CS microenvironment (and to a lesser extent to EDCM), whereas faster phosphorylation of PRAS40 in FBS, and of Akt (Ser473) in EDCM and 5-azacytidine occurs compared to CCM. For the first time, we demonstrated that the MSC pool held in the mediastinal fat is adequately plastic to partially differentiate in vitro toward a cardiac-like lineage. Besides, we have provided novel evidence of the potent inductive niche-like microenvironment that the CS structure can reproduce in vitro. hmADMSCs can represent an interesting tool in order to exploit their possible role in cardiovascular diseases and treatment.

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Study of Adipose Tissue-Derived Mesenchymal Stem Cells Transplantation for Rats with Dilated Cardiomyopathy

Cited by 14 publications

References 17 publications

Therapeutic Effect of Adipose Derived Stem Cells versus Atorvastatin on Amiodarone Induced Lung Injury in Male Rat

Therapeutic Effect of Adipose Derived Stem Cells versus Atorvastatin on Amiodarone Induced Lung Injury in Male Rat

Effects of the intramyocardial implantation of stromal vascular fraction in patients with chronic ischemic cardiomyopathy

Cardiosphere Conditioned Media Influence the Plasticity of Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells

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