Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Samadi, Nasser; Pakzad, Iraj; Sefidan, Alireza Monadi; Hosainzadegan, Hasan; Tanomand, Asghar

doi:10.5812/jjm.18102

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…The first isolation of an armA -harboring Salmonella strain occurred in 2011; fortuitously, no increasing trend for armA presence was found in the community-acquired infections in Shanghai. As with other clinical Enterobacteriaceae isolates ( Samadi et al, 2015 ), the armA prevalence rate in clinical Salmonella isolates from the present work was found to remain low over the study period. However, the presence of other aminoglycoside-resistance genes was not assessed, and antimicrobial susceptibility testing was not performed on all the isolated strains from this study, only the strains with armA , so strains with other aminoglycoside resistance mechanisms may have remained undetected.…”

Section: Discussionsupporting

confidence: 70%

Prevalence of 16S rRNA Methylation Enzyme Gene armA in Salmonella From Outpatients and Food

et al. 2021

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Salmonella is the primary cause of community-acquired foodborne infections, so its resistance to antimicrobials, such as aminoglycosides, is a public health issue. Of concern, aminoglycoside resistance in Salmonella is increasing rapidly. Here, we performed a retrospective study evaluating the prevalence of Salmonella harboring armA-mediated aminoglycoside resistance in community-acquired infections and in food or environmental sources. The prevalence rates of armA-harboring Salmonella strains were 1.1/1,000 (13/12,095) and 8.7/1,000 (32/3,687) in outpatient and food/environmental isolates, respectively. All the armA-harboring Salmonella strains were resistant to multiple drugs, including fluoroquinolone and/or extended-spectrum cephalosporins, and most (34/45) belonged to serovar Indiana. The armA gene of these strains were all carried on plasmids, which spanned five replicon types with IncHI2 being the dominant plasmid type. All the armA-carrying plasmids were transferable into Escherichia coli and Acinetobacter baumannii recipients. The conjugation experiment results revealed that the armA-harboring S. Indiana strains had a relatively higher ability to acquire armA-carrying plasmids. The low similarity of their pulsed field gel electrophoresis patterns indicates that the armA-harboring Salmonella strains were unlikely to have originated from a single epidemic clone, suggesting broad armA spread. Furthermore, the genetic backgrounds of armA-harboring Salmonella strains isolated from outpatients exhibited higher similarity to those isolated from poultry than to those isolated from swine, suggesting that poultry consumption maybe an infection source. These findings highlight an urgent need to monitor the prevalence and transmission of armA-harboring Salmonella, especially S. Indiana, to better understand the potential public health threat and prevent the further spread of these strains.

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Section: Discussionsupporting

confidence: 70%

Prevalence of 16S rRNA Methylation Enzyme Gene armA in Salmonella From Outpatients and Food

et al. 2021

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“…As well as, Stępień-Pyśniak et al in (2016) demonstrated that E. faecalis isolates absolute resistance 100% against erythromycin. Furthermore, Endo et al in (2014); Talebi et al in (2015); Samadi et al in (2015) and Pourcel et al in (2017) detected that 66.7% of isolates were resisting to azithromycin, 86% of isolates were resisting to tetracycline, 80% of isolates were resisting to amikacin and 77.2% of isolates were resisting to gentamicin, respectively;…”

Section: Antibiotics Susceptibility Testmentioning

confidence: 99%

Molecular and Bacteriological Study of Enterococcus faecalis Isolated from Different Clinical Sources

Khdir

Mustafa

2020

ZJPAS

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Enterococci are Gram-positive bacteria that cause serious nosocomial infections, including urinary tract, bloodstream infections and endocarditis. During the period of September 2018 to February 2019, forty four isolates of E. faecalis were isolated from 826 clinical specimens including; 35(5.07%) isolates of E. faecalis from urine, 7(7.60%) isolates from high vaginal swab and 2(8.69%) isolates from blood patients in different hospitals. All isolates that described above were identified depending on cultural criteria, morphological criteria, biochemical tests and further confirmed by Vitek 2 compact systems. The results of eleven antimicrobial against obtained isolates revealed that 100% of E. faecalis were resistant to cefotaxime, vancomycin, amoxicillin and erythromycin. While, it were 100% sensitive to doxycycline, imipenem, and nitrofurantion. Whereas, most isolates were differ in their susceptibility to amikacin, gentamycin, tetracycline and azithromycin. On the other hand, the results of biofilm found that 13.63% of isolates were produce strong biofilm, 54.54% were produce moderate biofilm and 31.81% were produce weak biofilm. The results of molecular analysis by using PCR showed that isolated E. faecalis were carried 97.72%, 90.90%, 63.63% of ebpR, asa1, esp genes respectively.

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“…Further, they showed that hMSCs differentiation toward chondrocytes was induced in the presence of higher concentrations of TGFβ-1 [92]. Polysaccharides derived from micro-organism sources have multiple industrial applications [93][94][95]. The advantages of this biomaterial include water-solubility, gelling capacity by lowering the temperature, and biological properties (such as non-cytotoxicity and cyto-compatibility) which make them suitable candidate for cartilage tissue engineering [96].…”

Section: Hyaluronanmentioning

confidence: 99%

The Use of Nanomaterials in Tissue Engineering for Cartilage Regeneration; Current Approaches and Future Perspectives

Eftekhari

Dizaj

Sharifi

et al. 2020

IJMS

109

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The repair and regeneration of articular cartilage represent important challenges for orthopedic investigators and surgeons worldwide due to its avascular, aneural structure, cellular arrangement, and dense extracellular structure. Although abundant efforts have been paid to provide tissue-engineered grafts, the use of therapeutically cell-based options for repairing cartilage remains unsolved in the clinic. Merging a clinical perspective with recent progress in nanotechnology can be helpful for developing efficient cartilage replacements. Nanomaterials, < 100 nm structural elements, can control different properties of materials by collecting them at nanometric sizes. The integration of nanomaterials holds promise in developing scaffolds that better simulate the extracellular matrix (ECM) environment of cartilage to enhance the interaction of scaffold with the cells and improve the functionality of the engineered-tissue construct. This technology not only can be used for the healing of focal defects but can also be used for extensive osteoarthritic degenerative alterations in the joint. In this review paper, we will emphasize the recent investigations of articular cartilage repair/regeneration via biomaterials. Also, the application of novel technologies and materials is discussed.

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Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Cited by 8 publications

References 29 publications

Prevalence of 16S rRNA Methylation Enzyme Gene armA in Salmonella From Outpatients and Food

Prevalence of 16S rRNA Methylation Enzyme Gene armA in Salmonella From Outpatients and Food

Molecular and Bacteriological Study of Enterococcus faecalis Isolated from Different Clinical Sources

The Use of Nanomaterials in Tissue Engineering for Cartilage Regeneration; Current Approaches and Future Perspectives

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