The development of material-based glucose sensor SiO<sub>2</sub>-CuO using Screen Printed Carbon Electrode (SPCE) had been done. Three types of materials were used to detect glucose, i.e Multi Wall Carbon Nanotube (MWCNT)-SiO2-CuO, SiO2-CuO (A) dan SiO2-CuO (B). The differences composition of SiO<sub>2</sub>-CuO(A) and SiO<sub>2</sub>-CuO(B) occurred during the addition of NaOH in synthesis process of SiO2-CuO (B). The prepared materials were analyzed by Scanning Electron Microscopy (SEM), cyclic voltammetry method and chrono-amperometry. Cyclic voltammetry analysis was conducted at a potential range of -1.0 - 1.5 V with Ag/AgCl as reference electrode. The scan rate was 100 mV/sec and the potential was varied at (-0.6), (-0.5) and (-0.4) V, in which the duration of each analysis was 5 second. Based on the result of analysis, whether by SEM, cyclic voltammetry and chrono-amperometry, the SiO<sub>2</sub>-CuO (B) was found to be the best material for detection of glucose.