Study of Antibacterial Activity of Chenopodium album Leaves Extract

Abstract

This study describes the antibacterial activities of three different solvent extracts of leaves of Chenopodium album. Methanol, acetone and chloroform extracts of C. album were prepared. The antibacterial activity was assessed using well plate method and were examined for the size of zone of inhibition. Different extracts were investigated against the test organisms namely Lactobacillus, Bacillus subtilis and Escherichia coli. The maximum activity was observed at 100% concentration of different extracts of leaves. The maximum zone of inhibition for 100% concentration were observed as E. coli (19 mm) and Lactobacillus (19 mm) in diameter respectively. C. album did not show any antibacterial activity against B. subtilis. Antibacterial activity was compared with standard Amoxicillin and it was found to be 23 mm diameter for Lactobacillus and 25 mm for both E. coli and B. subtilis in terms of zone of inhibition.

Ethnobotanical, Phytchemical, and Allelopathic Potentinal of Traditional Medicinal Plants

“…Saponin and protein were absent in Zanthoxylum armatum (Mehta et al, 2013). Main phytoconstituents such as tannins, carbohydrates, phenols, and alkaloids were found to be present while protein and flavonoid were absent in the methanolic leaf extracts of Chenopodium album, results are under the former studies (Kaur et al, 2018;Saini et al, 2019). The results of phytochemical analysis of Swertia chirayita showed the presence of various phytochemicals such as alkaloids, flavonoid, phenols, saponin, tannins and terpenoids (Kumar et al, 2015).…”

“…Test for Glycosides 1 ml of extract was allowed to react with 1 ml of concentrated Sulphuric acid; the formation of reddish colour when allowed to stand for 2 minutes validated the presence of glycoside (Lokman et al, 2013).…”

“…Test for Terpenoids Salkowaski Test: 5 drops of concentrated sulphuric acid were added in 2 ml of extract, shaken, and allowed to stand. The appearance of the greenish-blue colour indicated the presence of terpenoids (Lokman et al, 2013) Test for Saponins Olive Oil Test: 5 ml of extract was added to a few drops of olive oil. The appearance of froth indicated the presence of saponins (Gul et al, 2017).…”