Study of arsenic–protein binding in serum of patients on continuous ambulatory peritoneal dialysis

Zhang, Xinrong; Cornelis, Rita; Kimpe, Jürgen De; Mees, L.; Lameire, Norbert

doi:10.1093/clinchem/44.1.141

Cited by 29 publications

(32 citation statements)

References 15 publications

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“…SEC methods without ESI‐MS coupling were designed for the study of arsenic bindings of serum proteins. The separated protein fractions were collected and analyzed off‐line with atomic absorption spectroscopy (AAS) regarding their arsenic contents 14. An on‐line coupling of SEC with inductively coupled plasma mass spectrometry (ICP MS) was used for the arsenic determination in protein fractions from cardiovascular tissues 15…”

Section: Introductionmentioning

confidence: 99%

Size exclusion chromatography coupled to electrospray ionization mass spectrometry for analysis and quantitative characterization of arsenic interactions with peptides and proteins

2009

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Arsenic-binding proteins are of toxicological importance since enzymatic activities can be blocked by arsenic interactions. In the present work, a novel methodology based on size exclusion chromatography coupled to electrospray ionization mass spectrometry (SEC-ESI-MS) was developed with special emphasis to preserve the intact proteins and their arsenic bindings. The eluent composition of 25 mMTris/HCl, pH 7.5, with the addition of 100-mM NaCl optimized for SEC with UV detection provided the highest SEC separation efficiency, but was not compatible with the ESI-MS because of the non-volatility of the buffer substance and of the salt additive. In order to find the best compromise between chromatographic separation and ionization of the arsenic-binding proteins, buffer type and concentration, pH value, portion of organic solvent in the SEC eluent as well as the flow rate were varied. In the optimized procedure five different arsenic-binding peptides and proteins (glutathione, oxytocin, aprotinin, alpha-lactalbumin, thioredoxin) covering a molar mass range of 0.3-14 kDa could be analyzed using 75% 10-mM ammonium formate, pH 5.0/25% acetonitrile (v : v) as eluent and a turbo ion spray source operated at 300 degrees C and 5.5 kV. A complete differentiation of all peptides and proteins involved in the arsenic-binding studies as well as of their arsenic-bound forms has become feasible by means of the extracted ion chromatograms (XIC) of the mass spectrometric detection. The new method offered the possibility to estimate equilibrium constants for the reaction of phenylarsine oxide with different thiol-containing biomolecules by means of the XIC peak areas of reactants and products. Limits of detection in the range of 2-10 microM were obtained by SEC-ESI-MS for the individual proteins.

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Section: Introductionmentioning

confidence: 99%

Size exclusion chromatography coupled to electrospray ionization mass spectrometry for analysis and quantitative characterization of arsenic interactions with peptides and proteins

2009

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“…It was also reported that arsenicosis was more prevalent in households which consumed fewer protein intake in-terms of eating no egg or infrequent egg consumption. Poor nutrition, particularly less intake of protein resulting in decreased potentiality of methylation, consequently hampering the arsenic detoxification capacity and thus resulting in increased the toxicity [27,37,38].…”

Section: Discussionmentioning

confidence: 99%

Twenty Years of Arsenic Contamination and Arsenicosis Patients in a Village of Bangladesh

Shojaei¹,

Faruquee²,

Khan³

2020

AJPHR

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Background: Arsenic contamination in groundwater of Bangladesh was first detected in 1993 and still a major public health problem in the country. In 1997, Samta was identified as a severely arsenic contaminated village and many villagers were found to suffering from arsenicosis. Methods: A series of surveys were carried out in Samta village in the years 1997, 2002, 2008 and 2017 to assess the situation of arsenic contamination and the status of arsenicosis in the village. The water sources and the entire population of the village were included in the study. Results: In 1997 about 87% of the shallow tubewells in Samta village were arsenic-contaminated and 10.1% villagers had arsenicosis. Twenty years later in 2017, about 90% shallow tubewells were arsenic contaminated, 39 deep tubewells and a pond sand filter were installed in the village and 2.3% of the villagers had arsenicosis. On an average 43.8% arsenicosis patients had recovered from the illness and condition of 21.2% patients had deteriorated or remained unchanged. During the 20-year period 37(6.1%) arsenicosis patients and 185(1.6%) non-arsenicosis villagers had died. The main cause of death among arsenicosis patients was cancer (40.5%) and among non-arsenicosis villagers the cause was geriatric problem (11.8%). The mean age at death was 57.6 years and 61.2 years respectively. Conclusion: In twenty years, measures were undertaken to make available arsenic-safe water options and people were motivated to stop the use of arsenic-contaminated water and to increase the intake of protein and vitamin AEC rich food. Recovery had occurred in a substantial proportion of patients.

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“…The removal of absorbed As may be facilitated by diuresis, bile excretion, hemodialysis, or exchange transfusion (Kazantzis 2007). Arsenic is slowly bound to serum protein ranging from 5.3% (trivalent inorganic As) to 6.5% (pentavalent inorganic As) (Zhang et al 1998), but has a high volume of distribution (from 3.3 up to 4 l/kg) and rapidly passes from serum to tissues (its half-life in phase I occurs within 1-2 h, where about 90% As redistributes within 3 h) (Lai et al 2005).…”

Section: Chelation Therapy For Arsenic Poisoning: a Practical Approachmentioning

confidence: 99%

Arsenic intoxication: general aspects and chelating agents

et al. 2020

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Arsenic (As) is widely used in the modern industry, especially in the production of pesticides, herbicides, wood preservatives, and semiconductors. The sources of As such as contaminated water, air, soil, but also food, can cause serious human diseases. The complex mechanism of As toxicity in the human body is associated with the generation of free radicals and the induction of oxidative damage in the cell. One effective strategy in reducing the toxic effects of As is the usage of chelating agents, which provide the formation of inert chelator-metal complexes with their further excretion from the body. This review discusses different aspects of the use of metal chelators, alone or in combination, in the treatment of As poisoning. Consideration is given to the therapeutic effect of thiol chelators such as meso-2,3-dimercaptosuccinic acid, sodium 2,3-dimercapto-1-propanesulfonate, 2,3-dimercaptopropanol, penicillamine, ethylenediaminetetraacetic acid, and other recent agents against As toxicity. The review also considers the possible role of flavonoids, trace elements, and herbal drugs as promising natural chelating and detoxifying agents.

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Study of arsenic–protein binding in serum of patients on continuous ambulatory peritoneal dialysis

Cited by 29 publications

References 15 publications

Size exclusion chromatography coupled to electrospray ionization mass spectrometry for analysis and quantitative characterization of arsenic interactions with peptides and proteins

Size exclusion chromatography coupled to electrospray ionization mass spectrometry for analysis and quantitative characterization of arsenic interactions with peptides and proteins

Twenty Years of Arsenic Contamination and Arsenicosis Patients in a Village of Bangladesh

Arsenic intoxication: general aspects and chelating agents

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