Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns

Bárta, Jan; Čurn, Vladislav; Diviš, Jiří

doi:10.17221/4118-pse

Cited by 12 publications

(8 citation statements)

References 15 publications

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“…Native electrophoresis followed by the in‐gel activity assay of the ionically bound fractions revealed seven peroxidase isoenzymes in potato pulp protein extract (Figure a). The same number of peroxidase isoenzymes in potato pulp has been reported previously (Kurnik et al, ), and it corresponded to the composition of peroxidase family in the unprocessed potato tubers (Barta, Curn, & Divis, ; Préstamo & Manzano, ). This finding suggests that most potato peroxidases retain their activity after starch extraction from tubers.…”

Section: Resultssupporting

confidence: 84%

Study on utilizing solid food industry waste with brewers' spent grain and potato pulp as possible peroxidase sources

et al. 2017

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This study found that extracts containing ionically bound proteins, isolated from potato pulp and brewers' spent grain, were characterized by high peroxidase activities. The kinetic parameters, namely K m and V max values, were typical for plant peroxidases. Seven peroxidase isoenzymes in potato pulp and two isoenzymes in brewers' spent grain were obtained from their respective ionically bound fractions. Peroxidases from both potato pulp and brewers' spent grain displayed high storage stability, over a 90-day-long storage period, if stored at 2208C with glycerol added to a concentration of 50% or as unsupplemented extracts at 48C. Peroxidase activity was present in the covalently bound fraction of potato pulp, whereas it was absent in the respective fraction of brewers' spent grain. Covalently bound peroxidases from potato pulp displayed high activity, but low stability. Peroxidases extracted from brewers' spent grain and potato pulp, followed the pingpong mechanism and the sequence mechanism, respectively. Practical applicationsPlant peroxidases are widely applicable in various fields of biotechnology and diagnostics. These enzymes are used for biosensor and glucometer construction and in conjugation with antibodies for the purpose of enzyme immunoassays. Using waste products as a peroxidase source provides a cost-friendly alternative to commercially available horseradish peroxidase and gives an opportunity to recycle waste from the food industry-processed plant biomass. Here, the properties of peroxidases from food waste products, namely potato pulp and brewers' spent grain are analyzed. The extracts were characterized by high storage stability and high enzymatic activity, which are two key traits necessary for the practical use of enzyme preparations. K E Y W O R D Sbrewers' spent grain, enzyme storage stability, kinetic studies, peroxidase, potato pulp

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Section: Resultssupporting

confidence: 84%

Study on utilizing solid food industry waste with brewers' spent grain and potato pulp as possible peroxidase sources

et al. 2017

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“…The high stability of protein profile makes protein electrophoresis a powerful tool in elucidating the origin and the evolution of cultivated plants (Ladizinsky and Hymowitz, 1979), as well as fast and less expensive alternate tool (Smykal et al, 2008). Therefore, protein pattern analysis by gel electrophoresis has been used in higher plants to study various problems in genetics (Mohammed et al, 2006;Dakhil et al, 2013;Koshroo et al, 2011), taxonomy (Barta et al, 2003), physiology (Stegemann et al, 1987;Al-Helal, 1994), as it has been appreciated as a biochemical tool for studying the phylogentic relationships (Al-Yahyai and Al-Khanjari, 2008;Attaha, et al, 2013). The phylogenetic analysis based on protein patterns were used to study the genetic relatedness between and among cultivars (Abd El-Hady et al, 2010;Munshi and Osman, 2010;El Akkad, 2004;Haider et al, 2012;Khoshroo et al, 2011;Attaha et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic analysis of proteins from different date palm (Phoenix dactylifera L.) cultivars in Saudi Arabia

Mohammad¹

2015

Afr. J. Biotechnol.

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Fifteen (15) samples of different date palm cultivars were collected from different locations in Al-Ahsa oasis in the eastern province of Saudi Arabia. Extracted proteins from these samples were analyzed by electrophoresis, and clustered according to the average linkage between groups hierarchical clustering method. The results reveal high degree of similarity based on Jaccard`s similarity method on the basis of presence and absence of bands, that ranged between 0.421 to 0.917, which was represented by phylogenetic dendrogram in six clusters. The closely related cultivars "Hel" and "Hat" in addition to "Khl" represent the sixth cluster, which is separated out of other cultivars with high degree of similarity that ranged between 0.636 to 0.714; it was confirmed by the first principal component with high loading (52.3%), and characterized by four bands (92, 100, 205 and 108 kda). These bands were mostly positioned close to each other in the scatter diagram. The second principal component with loading of 15.7%, which were represented by three bands 19, 25, and 37 kda, have been confirmed the first cluster of closely related cultivars "Shi" and "Shl", as well as the closely related cultivars "Mj" and "OmR" among the second cluster. It can be concluded that most of Al-Ahsa oasis date palm cultivars were from one genetic origin, however, each cultivar was grown from seed of locally known cultivar, and later was selected due to preferred fruit characteristics. More biochemical and molecular studies would be necessary to uncover the genetic relationships between area cultivars.

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“…However, we were not able to ascribe the similarity of elec- BÁRTA et al (2003) studied the biochemical variability between thirteen European and five Czech potato (Solanum tuberosum L.) cultivars grown in the Czech Republic and confirmed that cultivar differences in protein polymorphism could be revealed by applied electrophoretic patterns. Our results also document the suitability of PAGE method recommended by UPOV (2002) could be stored in frozen condition for several months without any changes).…”

Section: Resultsmentioning

confidence: 62%

Characterisation of Potato (Solanum tuberosum L.) Varieties by Electrophoresis of Tuber Proteins

Sýkorová¹,

Matějová²

2006

CZECH J GENET PLANT

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Abstract:The modified PAGE method (TRIS -Glycine buffer pH 8.9) was used for the characterisation of selected 25 registered potato varieties. This method enabled to identify each variety from the examined variety set. The calculation of identity indexes (proportion of common bands) helped to evaluate the similarity of varieties from these aspects. The examination of electrophoretic profiles of soluble tuber proteins, which are highly polymorphic and stable, can be considered as valuable for variety characterisation and identification.

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Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns

Cited by 12 publications

References 15 publications

Study on utilizing solid food industry waste with brewers' spent grain and potato pulp as possible peroxidase sources

Study on utilizing solid food industry waste with brewers' spent grain and potato pulp as possible peroxidase sources

Electrophoretic analysis of proteins from different date palm (Phoenix dactylifera L.) cultivars in Saudi Arabia

Characterisation of Potato (Solanum tuberosum L.) Varieties by Electrophoresis of Tuber Proteins

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