Study of biochip integrated with microelectrodes modified by poly-dopamine-co-chitosan composite gel for separation, enrichment and detection of microbes in the aerosol

Shen, Xi; Ren, Rui; Wu, Yin; Li, Shifang; Ge, Changrong; Liu, Lulu; Xu, Yanhua

doi:10.1016/j.bios.2020.112931

Cited by 12 publications

(4 citation statements)

References 34 publications

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“…Microfluidic SERS chips are fabricated by integrating SERS-active nanostructures into microfluidic devices [ 115 , 116 ]. By making full use of the advantages of microfluidic chips and SERS, microfluidic SERS chips can not only provide highly reproducible SERS spectra for bacteria detection but also integrate different functional units for the separation and enrichment of target bacteria in complex fluid samples [ 117 ]. In recent years, with the rapid development of micro/nanofabrication technology, microfluidic SERS chips have shown great potential for the detection and identification of pathogenic bacteria and antimicrobial susceptibility testing (AST) [ 118 ].…”

Section: The Microfluidic Sers Chip For Bacteria Detection and Antimi...mentioning

confidence: 99%

Recent Progress of Surface-Enhanced Raman Spectroscopy for Bacteria Detection

Liu

Wang

et al. 2023

Biosensors

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There are various pathogenic bacteria in the surrounding living environment, which not only pose a great threat to human health but also bring huge losses to economic development. Conventional methods for bacteria detection are usually time-consuming, complicated and labor-intensive, and cannot meet the growing demands for on-site and rapid analyses. Sensitive, rapid and effective methods for pathogenic bacteria detection are necessary for environmental monitoring, food safety and infectious bacteria diagnosis. Recently, benefiting from its advantages of rapidity and high sensitivity, surface-enhanced Raman spectroscopy (SERS) has attracted significant attention in the field of bacteria detection and identification as well as drug susceptibility testing. Here, we comprehensively reviewed the latest advances in SERS technology in the field of bacteria analysis. Firstly, the mechanism of SERS detection and the fabrication of the SERS substrate were briefly introduced. Secondly, the label-free SERS applied for the identification of bacteria species was summarized in detail. Thirdly, various SERS tags for the high-sensitivity detection of bacteria were also discussed. Moreover, we emphasized the application prospects of microfluidic SERS chips in antimicrobial susceptibility testing (AST). In the end, we gave an outlook on the future development and trends of SERS in point-of-care diagnoses of bacterial infections.

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Section: The Microfluidic Sers Chip For Bacteria Detection and Antimi...mentioning

confidence: 99%

Recent Progress of Surface-Enhanced Raman Spectroscopy for Bacteria Detection

Liu

Wang

et al. 2023

Biosensors

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“…Certain conditions can weaken the immune system or modify the body's natural balance, allowing the yeast to uncontrollably grow and cause infection [36]. A clear influence of fungi activity in a biological system can be provided by changes in electron transport and membrane permeability of the yeast [37]. With all these considerations, this work has been devoted to exploring the optical, thermal, and electrical characteristics of C. albicans biofilms.…”

Section: Introductionmentioning

confidence: 99%

Multiphotonic Ablation and Electro-Capacitive Effects Exhibited by Candida albicans Biofilms

Arano-Martinez,

Hernández-Benítez,

Martines-Arano

et al. 2024

Bioengineering

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This work reports the modification in the homogeneity of ablation effects with the assistance of nonlinear optical phenomena exhibited by C. albicans ATCC 10231, forming a biofilm. Equivalent optical energies with different levels of intensity were irradiated in comparative samples, and significant changes were observed. Nanosecond pulses provided by an Nd:YAG laser system at a 532 nm wavelength in a single-beam experiment were employed to explore the photodamage and the nonlinear optical transmittance. A nonlinear optical absorption coefficient −2 × 10−6 cm/W was measured in the samples studied. It is reported that multiphotonic interactions can promote more symmetric optical damage derived by faster changes in the evolution of fractional photoenergy transference. The electrochemical response of the sample was studied to further investigate the electronic dynamics dependent on electrical frequency, and an electro-capacitive behavior in the sample was identified. Fractional differential calculations were proposed to describe the thermal transport induced by nanosecond pulses in the fungi media. These results highlight the nonlinear optical effects to be considered as a base for developing photothermally activated phototechnology and high-precision photodamage in biological systems.

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“…Since the majority of quick antigen screening kits lack quantitative features, their sensitivity dramatically declines when the viral load drops and the cycle threshold value exceeds 30 [2]. The Harvard University team made the initial suggestion for paper-based detection technology in 2009, for use in applications such as medical diagnostics, environmental monitoring, and food safety; their study proposed an altogether new concept of fluid handling and fluid analysis [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Innovative Cholesteric Nanostructure Biosensors on Paper Fibers for Disease Detection via Label-Free Approach and Image Analysis

Hsiao,

CHANG,

Chen

et al. 2023

Preprint

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We successfully achieved the synergistic development of a paper fiber-based biosensor that is label-free, flexible, portable, and color-indicating using chiral-nematic liquid crystals (LCs), also known as cholesteric LCs (CLCs). This method can be applied for quick screening in applications such as precision medicine. Optically active nematic LCs were layered in CLCs in various configurations and rotated around different axes. When light struck the LCs, the reflected light consisted of light of specific wavelengths. Using polarized optical microscopy and a charged couple device (CCD), we titrated bovine serum albumin (BSA) on the paper substrate and quantified the protein based on the observed color shift. Using internal Python pixel analysis software, we assessed the red, green, and blue color components that acted as biomarkers for different BSA concentrations. Results showed a linear relationship between the BSA concentration and color changes. This biosensor has the potential to quickly screen a large number of samples, increasing the effectiveness of illness identification in clinical settings. The CLC-based paper-substrate biosensor was developed using the color-indicating and measurable properties of CLCs, while also possessing the affordable and portable properties of paper substrate biosensors. With its easy, quick, and sensitive detection capabilities, disease detection may become more effective in the future.

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Study of biochip integrated with microelectrodes modified by poly-dopamine-co-chitosan composite gel for separation, enrichment and detection of microbes in the aerosol

Cited by 12 publications

References 34 publications

Recent Progress of Surface-Enhanced Raman Spectroscopy for Bacteria Detection

Recent Progress of Surface-Enhanced Raman Spectroscopy for Bacteria Detection

Multiphotonic Ablation and Electro-Capacitive Effects Exhibited by Candida albicans Biofilms

Innovative Cholesteric Nanostructure Biosensors on Paper Fibers for Disease Detection via Label-Free Approach and Image Analysis

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