Study of Biocontrol Properties of Bacillus Bacteria Genus in Relation to Carrot Phytopathogens in the Storage Process

Evaluating the Antagonistic Potential of Bacteria on Sclerotinia Slerotiorum, Causal Agent of White Mould of Carrots

“…Nine bacteria strains from the genera Pseudomonas and Bacillus were tested against S. sclerotiorum. In a controlled laboratory setting, P. fluorescens (F9-7) and B. subtilis (F9-2 and F9-12) inhibited the mycelial growth of S. sclerotiorum by up to 54%, which validates earlier in vitro research showing the potential of bacteria as an antagonistic agent (Expert & Digat, 1995;Pershakova et al, 2018). An inhibition of 36% (for day 7) was obtained when the experiment was conducted in vivo (on carrots), and when taking only the cell-free filtrate for the same three bacteria, the inhibition rate was 27% when considering a concentration of 15%.…”

“…In this study, the bacteria from the genus Bacillus belong to the B. subtilis species, which research has previously shown to be effective in controlling white mould disease on carrots during storage (Pershakova et al, 2018). All B. subtilis species were isolated from the same forestry disease suppressive compost as the P. fluorescens group strains and are comprised of three novel strains: F9-2, F9-8, and F9-12 (Mohamed et al, 2017).…”

“…To support the industry's need for more management options, this research explores how bacteria antagonistic to plant pathogens might offer a promising alternative to synthetic fungicides (Avis, 2007;Pershakova et al, 2018). Drawing from research by Mohamed et al (2017), the antagonistic effects, as well as the methods of action and putative bioactive compounds involved in two bacteria genera -Pseudomonas and Bacillusare explored to control S. sclerotiorum.…”

“…The work ofSun et al (2017) demonstrated that B. subtilis resulted in an inhibitory that was superior to 80%. The work ofPershakova et al (2018) showed that B. subtilis caused a delay in S. sclerotiorum growing in vitro. The work ofKamal et al (2015) also found that B. subtilis strongly inhibited mycelial growth with complete inhibition of sclerotial germination in vitro.Regarding the P. arsenicoxydans (F9-7) strain,Mohamed et al (2017) found that it was able to reduce the growth of both Fusarium sambucinum and Verticillium dahliae -two other mould phytopathogens.…”

Effects of different farming systems and crop protection strategies on the health status and yield of carrots Daucus carota L. ssp. sativus

“…effectively prevent microbiological damage to carrot roots [Patkowska and Błażewicz-Woźniak 2014]. In a study by Pershakova et al [2018], the application of Bacillus subtilis IMP 215 bacterial strain inhibited the growth of A. radicina on carrot roots. Asahi SL, Bio-Algeen 90, Kelpak SL and Tytanit biostimulants increased the marketable yield of carrots [Kwiatkowski et al 2013, Szczepanek et al 2015.…”