Study of Blood Porphyrin Spectral Profile for Diagnosis of Tumor Progression

Abstract

Renal cell carcinoma (RCC) accounts for approximately 3% of new cancer incidence and mortality in the United States. Unfortunately many RCC masses remain asymptomatic and nonpalpable until they are advanced. Diagnosis and localization of early carcinoma play an important role in the prevention and curative treatment of RCC. The autofluorescence of blood porphyrin of healthy and tumor induced in male SCID mice was analyzed using fluorescence and excitation spectroscopy. A significant contrast between normal and tumor blood could be established. Blood porphyrin fluorophore showed enhanced fluorescence band (around 630 nm) in function of the tumor growth. This indicates that either the autofluorescence intensity of the blood fluorescence may provide a good parameter for the "first approximation" characterization of the tumor stage.

Are brighter eggs better? Egg color and parental investment by House Wrens

“…Egg reflectance spectra exhibited peaks at approximately 630 and 700 nm (Fig. 2), consistent with the presence of the pigment protoporphyrin IX (Courrol et al 2007). We analyzed the spectra using a principal component analysis (PCA).…”

Stem cell-based photodynamic therapy

“…30 The autofluorescence of PpIX and heme is known to be a predictor of 75 cancer development, because the biosynthesis of porphyrins is upregulated in many cancers. [31][32][33] It is apparent that enhanced biosynthesis of porphyrins in B16F10 melanoma cells due to the presence of the precursor ALA increases the melanoma cell growth. Porphyrins are essential molecules within cells and have 80 multiple roles in essential cellular processes such as the mitochondrial electron transport chain, free-radical detoxification, and metabolic activity.…”

Optical redox ratio and endogenous porphyrins in the detection of urinary bladder cancer: A patient biopsy analysis

“…Many of the molecules present in human tissue naturally fluoresce, including amino acids (tryptophan ), structural proteins (collagen and elastin ), metabolic cofactors (NADH and flavins ) and blood precursors (porphyrins ). Tissue remodelling by tumours has previously been shown to significantly reduce local collagen levels to promote invasion and metastasis .…”

“…Research into the relationship between porphyrin levels and bladder cancer generally relates to their accumulation during PDD, therefore the profile of endogenous porphyrin in non‐sensitised tissue is poorly understood. Porphyrins have been shown to accumulate in oral cancer and in the blood and tissue of mice bearing renal cell carcinoma. We therefore considered that endogenous porphyrins may likewise accumulate in non‐sensitised human bladder tissue.…”