Study of calf thymus deoxyribonucleoproteins by means of gel electrophoresis, effect of ionic composition on the mode of chromatin fragmentation

Lishanskaya, A.I.; Mosevitsky, Mark I.

doi:10.1016/0006-291x(75)90396-4

Cited by 10 publications

(1 citation statement)

References 9 publications

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“…Metal ions are known to participate in stablization of numerous biological structures (31). Some metal ions, among which is Cu(ll) have been found to be directly bound to specific sites of nucleic acid part of chromatin (11,32) and thought to serve as linkers between nucleic acid and proteins. Even though DNA conformation has been suggested to determine the site-specificity of metal-ion binding to DNA, little is known about the structural properties of these binding sites due to lack of proper probes.…”

Section: Discussionmentioning

confidence: 99%

Site-specific cleavage of supercoiled DNA by ascorbate/Cu(II)

Wang¹,

Ness²

1989

Nucl Acids Res

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We have investigated ascorbate/Cu(II) cleavage of double-stranded DNA in the presence and absence of DNA negative torsion. We found that ascorbate/Cu(II) cleavage shows a site-specificity that is dependent on negative torsion and is influenced by the nature of the salt, ionic strength, and pH. This provides strong evidence for involvement of local DNA conformation in ascorbate/Cu(II) specific cleavage sites, that differs from the previous reports on cleavage of linear double-stranded DNA and secondary structures assumed by single-stranded DNA. The data indicate specific binding of Cu(II) ions to sites in the negatively supercoiled DNA. Fining mapping of the cleavage sites does not reveal any known DNA conformation, nor does it indicate any sequence identity among the sites cleaved. However, identification of a major site of cleavage of supercoiled DNA at physiological ionic strength, pH and temperature, along with fact that ascorbate and Cu(II) are normal cell constituents, suggests the torsion-dependent, site-specific interactions could have biological significance.

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Section: Discussionmentioning

confidence: 99%