Study of Catalase Enzyme in Methylotrophic Yeasts

Koleva, D.I.; Petrova, Ventsislava Yankova; Hristozova, Tz.; Kujumdzieva, Anna

doi:10.1080/13102818.2008.10817548

Cited by 8 publications

(5 citation statements)

References 32 publications

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“…This additional carbon source present on crude glycerol could explain the higher biomass yield obtained with this medium, since methanol is mostly evaporated during sterilization. The values of biomass yields of P. pastoris growing on glycerol range from 0.32 mass of cells per mass of substrate [25] and 0.51 mass of cells per mass of substrate [26] and even 0.86 mass of cells per mass of substrate [27], depending of the strain and the experimental conditions. The relatively high cell mass yields at 5 bar with crude glycerol, when compared to the medium with pure glycerol, point out the remarkable influence of the additional nutrients present in crude glycerol.…”

Section: Air Pressure Effect On Batch Culturesmentioning

confidence: 99%

Batch and fed-batch growth of Pichia pastoris under increased air pressure

Lopes

Belo

Мота

2012

Bioprocess Biosyst Eng

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Pichia pastoris CBS 2612 behavior under air pressures of 1, 3 and 5 bar in culture media of glycerol (pure and crude) and methanol was studied. Generally, the increase in oxygen transfer rate due to the increase of total pressure improved cellular growth for all carbon sources and for batch and fed-batch processes with different feeding rate strategies. In batch cultures, 1.4-, 1.2-, and 1.5-fold improvement in biomass production was obtained with the increase of air pressure up to 5 bar, using methanol, pure glycerol, and crude glycerol, respectively. The increase of air pressure to 5 bar using exponential feeding rate led to 1.4-fold improvement in biomass yield per glycerol mass consumed, for crude and pure glycerol. The current low cost of crude glycerol from the biodiesel production together with the present results shows the possibility of improving cell mass production of P. pastoris using increased air pressure.

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Section: Air Pressure Effect On Batch Culturesmentioning

confidence: 99%

Batch and fed-batch growth of Pichia pastoris under increased air pressure

Lopes

Belo

Мота

2012

Bioprocess Biosyst Eng

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“…Samples of the liquid cultures were centrifuged for 10 min at 1000 g at 4 °C (Koleva et al., 2008), and the supernatant was used as the crude enzyme preparation for fibrolytic enzyme activities using a microplate reader 680XR (Bio‐Rad, Hercules, CA, USA). An aliquot of suitably diluted enzyme solution was incubated with 2 vol of 100 μ m methyl ferulate in 100 m m 3‐(N‐morpholino) propane sulphonic buffer (pH 6.0) at 39 °C for 30 min, and the absorbance was estimated by microplate reader 680XR (Bio‐Rad) at 340 nm (recommended by Biocatalysts, Cardiff, Wales, UK).…”

Section: Methodsmentioning

confidence: 99%

“…Determination of pH and temperature optima and stability, cation stability and kinetic constants of FAE and AE After 5 days of incubation, when FAE and AE activities reached the highest level, the cultures were centrifuged for 10 min at 1000 g at 4°C (Koleva et al, 2008), and the supernatant was used as the crude enzyme preparation to analyse the enzymological characteristic of FAE and AE.…”

Section: Release Of Ferulic Acid and Vfas Analysesmentioning

confidence: 99%

Enzymatic characteristics of crude feruloyl and acetyl esterases of rumen fungus Neocallimastix sp. YAK11 isolated from yak (Bos grunniens)

Cao

Yang

Zhang

2012

Animal Physiology Nutrition

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Summary Rumen fungus Neocallimastix sp. YAK11 was isolated from yak (Bos grunniens), and three consecutive 10‐day pure cultures were anaerobically performed at 39 °C in 20‐ml Hungate’s tubes to explore ferulic acid esterase (FAE) and acetyl esterase (AE) activity profiles of the fungus grown on whole hay fraction of Chinese wildrye grass (Leymus chinensis) (WHOcw, n = 4) and its neutral detergent fibre fraction (NDFcw, n = 4), respectively. An aliquot of 0.7‐ml culture was sampled daily using a sterile syringe, and 0.7‐ml fresh medium was immediately added to the tubes to compensate for the withdrawn samples. Peak esterase activity occurred for FAE on day 5 (p < 0.001) and for AE on day 6 (p < 0.001). The mean activities of FAE and AE in WHOcw were 2.07 and 1.29 times of those in NDFcw (p < 0.001). Both FAE and AE activities were positively correlated with xylanase (r > 0.65, p < 0.001) and carboxymethyl cellulase (r > 0.57, p < 0.001) activities. Total volatile fatty acid concentration was positively correlated with enzyme activities of AE (r > 0.87, p < 0.001), FAE (r > 0.82, p < 0.001) and xylanase (r > 0.56, p < 0.001). Crude enzyme solution was harvested for the fungus grown on WHOcw, and the pH optimum of FAE activity was 8.0 while the optimum for AE was 9.0. Both FAE and AE had a broad pH stability range. The optimal temperatures for FAE and AE activity were 40 and 50 °C. The Michaelis constant (Km) and maximum velocity (Vmax) for FAE against methyl ferulate at pH 6.0 and 39 °C were 0.078 mm and 2.93 mU, respectively. The Km and Vmax for AE against p‐nitrophenyl acetate at pH 7.0 and 39 °C were 2.73 mm and 666.67 mU, respectively. Both FAE and AE may have prospective advantages for the enzymatic degradation of roughages in ruminant animals.

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“…This increase in the enzyme activity was probably due to the oxidation of the relatively toxic compound methanol, which proceeds with the formation of the two other very reactive compounds, formaldehyde and hydrogen peroxide. Taking into consideration the fact that superoxide dismutase neutralized superoxide anion to hydrogen peroxide, it is not surprising that high total catalase activity have also been measured in cell free extracts of methilotrophic yeasts grown on methanol containing medium (15). The utilization of methanol proceeds via ROS producing alcohol oxidase, located into peroxisomes, thus leading to active peroxisomal proliferation in yeast cells (18,30).…”

Section: Influence Of Carbon Source On Growth Of H Polymorpha and Somentioning

confidence: 98%

Superoxide Dismutase Enzymes in Oxidative Type YeastH. Polymorpha

Koleva

Docheva

Petrova

et al. 2010

Biotechnology & Biotechnological Equipment

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Hansenula polymorpha CBS 4732, cultivated on media containing glucose, glycerol or methanol as carbon source has been investigated for specific activity of Superoxide dismutase (SOD) enzymes during batch -wise cultivation. Furthermore cell free extract from the analyzed strain cultivated on different carbon sources were subjected to 8% native PAGE. One band corresponding to sensitive of KCN Cu/Zn SOD with Rm 0.29 is inhibiting in all samples. After inhibition of cyanide sensitive Cu/Zn SOD, three bands specifically stained for SOD activity, corresponding to Mn SOD are observed in samples obtained by H. polymorpha cultivated on medium containing methanol. Two bans are retaining in glucose and glycerol grown cells. This data suggest that probably in H. polymorpha yeasts appears multiple enzyme forms of Mn SOD enzyme. Multiple enzyme forms become more stable and do not disappear after 1 min at 75°C. One band with Rm 0.34 in all yeast samples cultivated on methanol, glucose and glycerol, remains till heating at 75°C for 10 minutes.

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Study of Catalase Enzyme in Methylotrophic Yeasts

Cited by 8 publications

References 32 publications

Batch and fed-batch growth of Pichia pastoris under increased air pressure

Batch and fed-batch growth of Pichia pastoris under increased air pressure

Enzymatic characteristics of crude feruloyl and acetyl esterases of rumen fungus Neocallimastix sp. YAK11 isolated from yak (Bos grunniens)

Superoxide Dismutase Enzymes in Oxidative Type YeastH. Polymorpha

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