Study of charge distribution on the surface of biocolloids

Dziubakiewicz, Ewelina; Hrynkiewicz, Katarzyna; Walczyk, M.; Buszewski, Bogusław

doi:10.1016/j.colsurfb.2012.11.018

Cited by 22 publications

(22 citation statements)

References 40 publications

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“…It results from solvatation and (de) protonation processes onto bacterial surface [7]. Intensive absorption bend of carboxyl and amides groups prove formation controlled bacterial aggregates with divalent ions during electrofocusing [6]. Moreover, obtained results appear dominant contribution of carboxyl and amino groups in solvatation processes, charge distribution, biocolloids dispersion and in consequences stability of system [6,7].…”

Section: Ftir Spectroscopymentioning

confidence: 81%

“…Qualitative changes in the composition of cells translates directly into the nature of the registered spectrum. Obtained results suggest use of MALDI TOF MS like selective detector in electroseparation process of bacterial cells using capillary zone electrophoresis approach [6,10]. Application of HCCA and DHB matrix gave reproducible spectra with high resolution and stable ionization.…”

Section: Spectrometric Analysis Of Bacteriamentioning

confidence: 98%

“…Obtained results suggest use of MALDI TOF MS like selective detector in electroseparation process of bacterial cells using capillary zone electrophoresis approach [6,10].…”

Section: Spectrometric Analysis Of Bacteriamentioning

confidence: 99%

“…Its proves dominant contribution of carboxyl and amino groups in protonationdeprotonation process taking place at the interface of bacterial surface. This information is helpful in optimization of electroseparation process of bacterial cells [6]. (Figures 4 and 5) Figure 1B and 1C.…”

Section: Ftir Spectroscopymentioning

confidence: 99%

“…Macromolecules that are present in the cell wall and bacterial membranes produce unique biochemical fingerprints and contribute to the surface charge of bacterial cells through the ionization of protonactive functional groups, such as carboxyl, phosphate, amino, and hydroxyl groups [6,7]. According to electrokinetic theory, the discussed active center (functional groups) of macromolecules play a crucial role in electroseparation process during capillary zone electrophoresis and capillary isoelectric focusing of bacterial cells and determine nature of microorganisms [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Assignment of functional groups in Gram-positive bacteria

Buszewski¹

2015

J Anal Bioanal Tech

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The chemical composition, molecular structure and physicochemical properties of five Gram-positive bacterial strain: Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Micococcus luteus were investigated by Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), NMR spectroscopy and intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (IC MALDI TOF MS). An analysis of FTIR spectra as a function of pH revealed the three major types of cell wall functional groups -carboxyl group, amino group and phosphate group. An analysis of XPS spectra was determinate the major surface components of bacterial cell.13 C NMR and IC MALDI TOF MS spectra of six bacterial species were registered. Our findings indicate that chemical and structural differences in the cell composition of Gram-positive bacteria can be detected.The obtained results also demonstrate that the combination of FTIR, XPS and NMR spectroscopy with IC MALDI TOF MS technique yields useful information and complements other biochemical and physical methods of microbial cells characteristics.

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Section: Ftir Spectroscopymentioning

confidence: 81%

Section: Spectrometric Analysis Of Bacteriamentioning

confidence: 98%

“…Obtained results suggest use of MALDI TOF MS like selective detector in electroseparation process of bacterial cells using capillary zone electrophoresis approach [6,10].…”

Section: Spectrometric Analysis Of Bacteriamentioning

confidence: 99%

Section: Ftir Spectroscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assignment of functional groups in Gram-positive bacteria

Buszewski¹

2015

J Anal Bioanal Tech

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Capillary electrophoresis of microbial aggregates

Dziubakiewicz

Buszewski

2014

Electrophoresis

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Uncontrolled aggregation of bacterial cells is a significant disadvantage of electrophoretic separations. Various aspects of the electrophoretic behavior of different strains of Gram-positive Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus(1), and Micrococcus luteus bacteria and Gram-negative Escherichia coli bacteria were investigated in this study. Our findings indicate that bacteria can be rapidly analyzed by CZE with surface charge modification by calcium ions (Ca(2+)). Bound Ca(2+) ions increase zeta potential to more than 2.0 mV and significantly reduce repulsive forces. Under the above conditions, bacterial cells create compact aggregates, and fewer high-intensity signals are observed in electropherograms. The above can be attributed to the bridging effect of Ca(2+) between bacterial cells. CE was performed to analyze bacterial aggregates in an isotachophoretic mode. A single peak was observed in the electropherogram.

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Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae Using Capillary Electrophoresis Approach

Pomastowski

2016

Capillary Electrophoresis

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Rapid detection and identification of microorganisms is a challenging and important aspect in many areas of our life, beginning with medicine, ending with industry. Unfortunately, classical methods of microorganisms identification are based on time-consuming and labor-intensive approaches. Screening techniques require rapid and cheap grouping of bacterial isolates; however, modern bioanalytics demands comprehensive bacterial studies on molecular level. The new approach to the rapid identification of bacteria is to use the electromigration techniques, especially capillary zone electrophoresis (CZE). CZE is an important technique used in the analysis of microorganisms. However, the analysis of microbial complexes using this technology still encounters several problems-uncontrolled aggregation and/or adhesion to the capillary surface. One way to resolve this issue is the CZE analysis of microbial cell with surface charge modification by bivalent metal ions (e.g., Ca(2+) aq, Zn aq). Under the above conditions, bacterial cells create compact aggregates, and fewer high-intensity signals are observed in electropherograms. The chapter presents the capillary electrophoresis of microbial aggregates approach with UV and one-dimensional intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (ICM MS) detection.

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Study of charge distribution on the surface of biocolloids

Cited by 22 publications

References 40 publications

Assignment of functional groups in Gram-positive bacteria

Assignment of functional groups in Gram-positive bacteria

Capillary electrophoresis of microbial aggregates

Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae Using Capillary Electrophoresis Approach

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