Background: Thyroid diseases may be complicated by anti-thyroglobin and anti-thyroid peroxidase antibodies. Objective: This research examined thyroid diseases and serum Anti-TPO Ab and Anti-Tg Ab levels. Methods: Al-Fadel foundation for training and development (research foundation) in Babylon province conducted this cross-sectional study. The research comprised 90 patients aged 15–45, separated into three groups: 60 patients with thyriodism, 30 healthy participants as a control group, and all patients (n=60) into two groups: hypothyroidism and hyperthyroidism. Results: Hyperthyroidism and hypothyroidism patients and healthy controls had similar mean Anti TPO levels. Compared to healthy controls, hypothyroidism and hyperthyroidism patient groups increased significantly. Hypothyroidism and hyperthyroidism patients had higher Anti Tg levels than the health control group. Conclusion: Anti-TOP Ab and Anti-Tg Ab vary significantly between Hyperthyroidism, Hypothyroidism, and control. Clinical labs should evaluate thyroidism patients for potential consequences using these biomarkers.