Study of DNA-Binding Activity and Antibacterial Effect of Escitalopram Oxalate, an Extensively Prescribed Antidepressant

Abstract

Escitalopram oxalate (EO) is considered as one of the extensively prescribed antidepressant drug in Turkey and some other countries, therefore this research was aimed to study the interaction of the drug with DNA and study of the substance effect on bacterial growth. The absorption value of the drug solution at 238 nm was increased when DNA was added gradually to it and it showed hyperchromism effect. The value obtained for DNA binding constant (Kb) was 0.035 M −1. When we added the CuCl2 2H2O to the mixture, any breakage was not shown in double strand DNA in comparison with control DNA. In addition low concentration of EO couldn’t protect DNA (0.5273 µmole bp) against Hydroxyl free radical (0.12 µmole) although it could protect the DNA when it was at the same or higher concentrations (0.5273, 5.273 and 252.73 µmole) than the DNA concentration. In addition, MIC of the drug for E.coli and Bacillus subtilis was almost 0.185 mM and 0.55 mM respectively. The E.coli strain was killed at concentrations 45, 15, 5 mM while the Bacillus subtilis was stable against all of the concentrations.

Evaluation of dexamethasone and its combination with mineral suppliments on the DNA compactness/breakage of antioxidant defense enzymes

“…In this research, all of ve hydrogen bonds shown in Dexamethasone-DNA docked model had a donor-acceptor distance ranging from 2.62 to 3.07 A˚ and one of them had 3.27 A˚. DNA in a human cell undergoes several thousand to a million damaging events per day, generated by both external (exogenous) and internal metabolic (endogenous) processes (Valipour et al, 2019). In order to explore DNA damage caused by chemicals, one of the inexpensive, rapid, and valuable method is incubation of a supercoiled plasmid DNA with the selected chemical followed by agarose gel electrophoresis( ) was as 5.12-21.03 nmol/L and 2.5-509.6 nmol/L, respectively.…”

“…UVvisible spectroscopy is the most common method to study drug-DNA interaction. In the spectrum of DNA hypochromic effects occurred due to changes in the conformation and structure of DNA, after binding of a drug to DNA(Valipour, Yilmaz, & Valipour, 2019). A hyperchromic effect is due to the binding of chemicals to DNA which might result in the unwinding of DNA helix(Tabassum, Chandra Sharma, Arjmand, & Azam, 2010).…”

In vitro and in silico analysis of the effect of fluconazole, an antifungal drug, on DNA

“…UV-visible spectroscopy is the most common and convenient way to study the interaction between chemicals and double stranded DNA. 35 The band at 260 nm of DNA arises due to the π-π* transitions of DNA bases. "Hyperchromic" effect and "hypochromic" effect are the spectra features of DNA due to its double helical structure.…”

Effect of Bezafibrate on Deoxyribonucleic Acid in the Presence of Iron and Copper

“…One of the inexpensive, fast and valuable methods to study the DNA-breaking activity of chemicals, is the treatment of plasmid DNA with the chemicals and analyze it in agarose gel electrophoresis (21,22). The native conformation of pUC-19 plasmid DNA is supercoil.…”