Study of electron spin resonance and viscosity for hemoglobin polymer after arsenic trioxide and gamma irradiation treatment

Saad-El-Din, Aisha A.; El-Tanahy, Z. H.; El-Sayed, Suzan N.; Anees, Laila M.; Farroh, Hoda A.

doi:10.1016/j.jrras.2014.07.010

Cited by 4 publications

(1 citation statement)

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“…Some investigators have reported alterations in the hematological parameters after exposure to irradiation [ 52 ], some of which are in harmony with the present investigation. RBC count and Hb values were depressed post whole-body γ-radiation in the dose-range 5–10 Gy [ 53 , 54 ]. The depression observed in RBCs, Hb and PLTs could be attributed to destruction of erythrocyte precursors in the bone marrow [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of polysaccharide fromAlcaligenes xylosoxidansMSA3 administration as protection against γ-radiation in female rats

et al. 2015

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Damage to normal tissues is a consequence of both therapeutic and accidental exposures to ionizing radiation. A water-soluble heteropolysaccharide called AXEPS, composed of glucose, galactose, rhamnose and glucouronic acid in a molar ratio of nearly 1.0:1.6:0.4:2.3, respectively, was isolated from culture medium of strain Alcaligenes xylosoxidans MSA3 by ethanol precipitation followed by freeze-drying. Chemical analysis, Fourier-transform infrared (FTIR) and chromatographic studies revealed that the molecular weight was 1.6 × 104 g mol−1. This study was designed to investigate the radioprotective and biological effects of AXEPS in alleviating the toxicity of ionizing radiation in female albino rats. A total of 32 female albino rats were divided into four groups. In the control group, rats were administered vehicle by tube for four weeks. The second group was administered AXEPS (100 mg/kg) orally by gavage for four weeks. Animals in the third group were exposed to whole-body γ-rays (5 Gy) and remained for 2 weeks without treatment. The fourth group received AXEPS (100 mg/kg) orally by gavage for two weeks before being exposed to whole-body γ-rays (5 Gy), then 24 h post γ-rays, they received AXEPS (100 mg/kg) in a treatment continuing till the end of the experiment (15 days after the whole–body γ-irradiation). Oral administration of AXEPS (100 mg/kg) significantly reversed the oxidative stress effects of radiation, as evidenced by the decrease in DNA damage in the bone marrow. Assessment of apoptosis and cell proliferation markers revealed that caspase-3 significantly increased in the irradiated group. Moreover, a significant decrease in the hematological constituents of peripheral blood, the chemotactic index and CD8+ T cells were observed in animals in the irradiation-only group, whereas an increase in the lymphocyte index was observed in animals in that group. In contrast, AXEPS treatment prevented these alterations. From our results, we conclude that AXEPS is a potent antioxidant and treatment agent for protection from γ-rays.

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