Study of Enumeration of Microorganisms in Food Samples with Automated Enumeration System, TEMPO

Kobayashi, Asuka; Okuyama, Satoko; Kenmotsu, Kesao; Yamagata, Hisashi; Uchida, Kazuyuki; Fukumura, Kazuya; Nakagawa, Hiroshi

doi:10.5803/jsfm.25.120

Cited by 2 publications

(5 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have shown that there is a remarkable correlation between the TEMPO and standard methods in various naturally contaminated samples at the same culture conditions (Paulsen et al. 2006, 2008; Kunicka 2007; Kobayashi et al. 2008; Torlak et al.…”

Section: Discussionmentioning

confidence: 97%

“…The automated TEMPO system has been developed to replace the disadvantages of the traditional MPN method. Previous studies have shown that there is a remarkable correlation between the TEMPO and standard methods in various naturally contaminated samples at the same culture conditions (Paulsen et al 2006(Paulsen et al , 2008Kunicka 2007;Kobayashi et al 2008;Torlak et al 2008;Crowley et al 2009Crowley et al , 2010Owen et al 2010). Therefore, the culture condition on the detection might not influence the experimental data even if there might be some difference in growth rate and the injury of bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…TEMPO is an automated system based on the most probable number (MPN) method for enumeration of aerobic bacteria and specific pathogens in foods (Paulsen et al. 2006, 2008; Kunicka 2007; Kobayashi et al. 2008; Torlak et al.…”

Section: Introductionmentioning

confidence: 99%

“…TEMPO is an automated system based on the most probable number (MPN) method for enumeration of aerobic bacteria and specific pathogens in foods (Paulsen et al 2006(Paulsen et al , 2008Kunicka 2007;Kobayashi et al 2008;Torlak et al 2008;Crowley et al 2009Crowley et al , 2010Owen et al 2010). It is seen as an internationally recognized method validated by EN ISO 16140 and the AOAC Research Institute and has been evaluated in various kinds of food matrices (Crowley et al 2009(Crowley et al , 2010Jasson et al 2010).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Enumeration of micro-organisms in processed soy products with an automated most probable number method compared with standard plate method

Katase

Tsumura

2011

Letters in Applied Microbiology

View full text Add to dashboard Cite

Aim: The automated TEMPO system (bioMerieux) is based on the most probable number (MPN) method for the enumeration of micro‐organisms in foods. In this study, we evaluated the performance of the TEMPO system as a diagnostic tool in comparison with the standard method in processed soy products. Methods and Results: A verification study was conducted using artificially contaminated soy product samples such as soy protein isolate, water‐soluble soy polysaccharides, soy milk and processed soy food. Five types of micro‐organisms were analysed using the automated MPN method (total aerobic bacteria, total coliforms, Enterobacteriaceae, yeast and mould and Staphylococcus aureus) vs the standard plate method. The results from each of the methods were highly correlated (r > 0·95). Naturally contaminated processed soy products on the market were also studied. There were no discrepancies observed between the respective methods. Conclusions: TEMPO methods were equivalent to the corresponding standard plate methods with very good rates of agreement. Significance and Impact of the Study: The automated MPN method is more practical and reliable for in‐house microbiological testing in processed soy products.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enumeration of micro-organisms in processed soy products with an automated most probable number method compared with standard plate method

Katase

Tsumura

2011

Letters in Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…However, MPN method is labor intensive and time consuming. TEMPO is an automated enumeration system based on the MPN method for bacterial enumeration in food samples (Kobayashi et al, 2008;Kunicka, 2007;Owen et al, 2010;Paulsen et al, 2006;Paulsen et al, 2008: Torlak et al, 2008). It is an internationally recognized method validated by ISO and the AOAC, and evaluated in various foods (Crowley et al, 2009;Crowley et al, 2010;Jasson et al, 2010).…”

Section: Automated Enumeration System Based On the Most Probable Number (Mpn) Methodsmentioning

confidence: 99%

Rapid Detection and Identification Systems for the Microbiological Assessment of Processed Soy Foods: A Review

Katase¹,

Tsumura²

2020

JFR

View full text Add to dashboard Cite

Plant-based diets are gaining interest in promoting physical and environmental health worldwide. The widely growing consumption of processed soy foods results in an increased demand for safe and high quality soy foods. Many of the rapid bacterial detection methods currently available are inhibited by components in the food matrixes. In recent years, high-throughput devices have been developed, which aid in the enumeration and evaluation of microorganisms in processed soy foods (automated fluorescent filter method, high-throughput identification using matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and automated most probable number method). These methods are more rapid and convenient compared to the conventional culture method. This review discusses alternate reliable methods for the microbiological assessment of processed soy foods, which guarantees the safety of the food delivered for consumption.

show abstract

Study of Enumeration of Microorganisms in Food Samples with Automated Enumeration System, TEMPO

Cited by 2 publications

References 1 publication

Enumeration of micro-organisms in processed soy products with an automated most probable number method compared with standard plate method

Enumeration of micro-organisms in processed soy products with an automated most probable number method compared with standard plate method

Rapid Detection and Identification Systems for the Microbiological Assessment of Processed Soy Foods: A Review

Contact Info

Product

Resources

About