Study of extraction and HPTLC - UV method for estimation of caffeine in marketed tea (<i>Camellia sinensis</i>) granules

Misra, H. O.; Mehta, Darshana; Mehta, B. K.; Soni, Marshal; Jain, D. C.

doi:10.4103/0973-8258.49374

Cited by 12 publications

(1 citation statement)

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“…A variety of analytical techniques and sample preparation procedures have been developed and registered for determination and separation of these three methylxanthines in plants, environmental samples, drugs, food, and biological fluids. These proposed methods include UV-Vis spectrophotometry [22–24], thin-layer chromatography (TLC) [25], ion chromatography (IC) [26], FT-Raman spectrometry [27], IR spectrometry [28], capillary electrophoresis (CE) [29, 30], NMR spectroscopy [31], mass spectroscopy [32], gas chromatography-mass spectrometry (GC-MS) [15], high-performance thin-layer chromatography (HPTLC) [33, 34], voltammetry [35, 36], and high-performance liquid chromatography (HPLC) which was reported as the most frequently used approach [1, 2, 10, 37–49].…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Determination of Methylxanthines in Commercial Brands of Tea Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry

Aqel

Al-Mulla

Al-Rifai

et al. 2019

International Journal of Analytical Chemistry

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Recently, chromatographic techniques have the potential to be greener in order to reduce the environmental impact. In this work, a new simple, sensitive, efficient, and green analytical method based on UHPLC-MS has been developed for a quick determination of methylxanthines including caffeine, theobromine, and theophylline in tea. Under the optimum conditions, a baseline separation has been achieved within 30 seconds, using isocratic elution consisting of 90% water and only 10% acetonitrile at 0.5 mL/min flow rate (3 mL acetonitrile per hour). The mass spectrometer was operated with the SIR mode in ESI+. The developed method was found to be linear in the range of 0.03–5 μg/mL, with correlation coefficients greater than 0.9995 for the three compounds. The respective values of LOD were found to be 0.025, 0.015, and 0.01 μg/mL for caffeine, theobromine, and theophylline, respectively. The proposed assay was applied to 30 commercial tea samples of different brands. Both caffeine and theobromine were found in all tea samples with maximum concentration in sample no. 15, corresponding to 32.6 and 2.72 mg/g of caffeine and theobromine, respectively. On the contrary, theophylline was not detected at all in most samples. When compared with all previous studies that dealt with the same compounds in different matrices, the developed method was found to be the fastest, allowing high-throughput analyses with more than 100 samples/h. The results prove that the method is suitable for routine analysis of methylxanthines and to distinguish the quality of tea samples of various brands.

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