Study of growth parameters and potentialities of differentiation of multipotent mesenchymal stromal cells from rat bone marrow in vitro

Abstract

The dynamics of growth and proliferative activity of the population of multipotent mesenchymal stromal cells from rat bone marrow was studied during 7 passages. The efficiency of colony formation, the morphology of multipotent mesenchymal stromal cells, and the possibility of spontaneous and induced differentiation were studied. The rat bone marrow fibroblast-like multipotent mesenchymal stromal cells are capable of clonal growth; their proliferative activity and the yield remained high until passage 4, but then decreased. Induction of osteo- or adipogenic differentiation of bone marrow multipotent mesenchymal stromal cells increased the percentage of morphologically modified cells carrying specific markers.

Experimental therapy of graft-versus-host disease by mesenchymal stromal cells grown on oxide nanocoatings

“…The adult MSCs are increasingly being used in clinical trials too [4]. Adult BM stroma contains a small amount of MSCs, which could be expanded in culture in vitro and reveal the ability to differentiate into osteo-, adipo-and chondrogenic directions [5]. About 10 years ago the MSCs grown in vitro were used for treatment of immune conflicts, particularly GVHD [5].…”

“…Adult BM stroma contains a small amount of MSCs, which could be expanded in culture in vitro and reveal the ability to differentiate into osteo-, adipo-and chondrogenic directions [5]. About 10 years ago the MSCs grown in vitro were used for treatment of immune conflicts, particularly GVHD [5]. It was shown that the long-term in vitro culturing significantly reduced the multilinear differentiation and homing potential of MSCs [6].…”

“…One of the methodological approaches to solve this task is co-transplantation of bone marrow and MSCs [4]. The MSCs are known to have a high potential of self-renewal and the ability to multilineage differentiation [5]. This functional status of MSCs is implemented both in vitro and in vivo during their migration and subsequent regeneration of the recipient damaged sites.…”

“…After 24 hrs the non-adhered cells were removed and an adhesive fraction was cultured till the subconfluent layer appearance; the growth medium was changed every 3 days. The cells were then detached with 0.25% Trypsin (Sigma-Aldrich, USA) and 0.02% EDTA (ICN, USA) solution according to the standard Experimental therapy of graft-versus-host disease by mesenchymal stromal cells grown on oxide nanocoatings procedure [5]; enzyme was inactivated with a culture medium containing 10% FBS; cells were then washed and counted. Thereafter the MSCs were passaged with an explantation density of 0.5-1x10 4 cells/cm 2 .…”