Study of Helminth Parasites of Amphibians by Scanning Electron Microscopy

González, Carmen; Hamann, Mónika Inés; Salgad, Cristina

doi:10.5772/35371

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…To study the ultrastructural features of trematodes, we found that the freshness of sample is critical and cannot be prolonged in the refrigerator longer than one day. Also, to allow the adult worm to be more relaxed, the fixation process should be performed with warm fixative (González et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Morphology and Ultrastructural Characteristics of Platynosomum fastosum Kossack, 1910 Adults and Eggs

2023

Int J Vet Sci

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Infection with Platynosomum fastosum, a liver fluke of domestic cats, can induce biliary obstruction and has been found to be associated with cholangiocarcinoma. To evaluate any new rational drugs, fundamental knowledges of parasite morphology are crucial but detailed morphology has not yet been investigated. Macroscopic, microscopic, and ultrastructural morphology of P. fastosum adult worms and eggs were evaluated. The adult fluke was reddish brown with a tamarind leaf-like body shape, 4.5mm in length and 1.5mm in width. Under scanning electron microscope (SEM), the tegumental surface of P. fastosum was spineless and covered with slender villous-like projections. Four types of papillae were found, and all were non-ciliated. P. fastosum fluke eggs were dark brown with an operculum and had a smooth eggshell surface with an inconspicuous posterior abopercular knob. From both fecal and bile samples, at least 3 types of eggs were present. The average size (mean±SD) of a typical P. fastosum egg was 44.2+1.7m in length and 31.8+2.2m in width. Under transmission electron microscope (TEM), tegument syncytium was composed of three layers. The first layer was an outermost trilaminate membrane covered by a glycocalyx. The second layer was tegumental cytoplasm composed of 2 types of tegumental granules, lysosomes, mitochondria and microtrabeculae. The third layer attached to a basal extracellular matrix with numerous basal membrane infoldings. This study revealed the first ultrastructural characteristics of P. fastosum adult and egg as a fundamental knowledge to use as basic criteria for assessment of anti-parasitic drug development in the future.

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Section: Discussionmentioning

confidence: 99%

Morphology and Ultrastructural Characteristics of Platynosomum fastosum Kossack, 1910 Adults and Eggs

2023

Int J Vet Sci

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“…Adult parasite specimens collected from some snake cavities/organs (see necropsy procedure, below) were fixed in hot 4% formaldehyde, dehydrated in an ascending series of ethanol solutions to 70%, transferred to 5% glycerin–95% ethanol from which the alcohol was allowed to evaporate, and cleared in 96.6% glycerol–0.05% potassium chloride–3.35% distilled water for 24–48 h prior to examination. Subsequently, specimens were mounted on metal specimen stubs with Duco cement, outgassed in a vacuum evaporator for 1 h, and rotary-coated with a thin layer of gold-palladium (Denton Vacuum Desk II; González et al, 2012) for examination by scanning electron microscopy (SEM) using a JEOL 5800 LV SEM at an accelerating voltage of 15 Kv. We used the descriptions of Brusca et al (2018), Heymons and Graf Vitzthum (1935), Rego (1983), and Christoffersen and De Assis (2013) to identify pentastomids, Artigas de Toledo and Paulino (1988) to identify digeneans, Slapeta et al (2003) for coccidians, and Vicente et al (1993) for nematodes.…”

Section: Methodsmentioning

confidence: 99%