Study of Heparin in Intestinal Ischemia and Reperfusion in Rats: Morphologic and Functional Evaluation

Ghadie, Majid Mohamad; Miranda-Ferreira, Regiane; Taha, Nabiha Saadi Abrahão; Maroso, A. S.; Moreti, R. J. Z.; Andraus, Mariela P.; Zempulski, P.; Monteiro, Hugo P.; Simões, Manuel de Jesus; Fagundes, Djalma José; Caricati‐Neto, Afonso; Taha, Murched Omar

doi:10.1016/j.transproceed.2012.07.055

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…Heparin administration has been demonstrated to limit IR injury to the kidney, intestine, nervous system, pancreas, lungs, liver and heart (Yagmurdur et al ., ; Mocco et al ., ; Taha et al ., ; Yurtcu et al ., ; Gedik et al ., ; Ghadie et al ., ; Warzecha et al ., ), leading us to hypothesise that heparin would have similar protective effects in testicular torsion injury. Our results demonstrate that heparin administration is associated with reduced PC and MDA accumulation in the ipsilateral testicle relative to untreated torsion–detorsion injury.…”

Section: Discussionmentioning

confidence: 95%

“…Previous studies have demonstrated that antioxidant enzymes and ROS‐scavenging compounds can prevent IR injury in experimental testicular torsion (Yagmurdur et al ., ; Can et al ., ; Dokmeci et al ., ; Mocco et al ., ; Taha et al ., ; Yurtcu et al ., ; Wei et al ., ; Gedik et al ., ; Ghadie et al ., ; Warzecha et al ., ; Zavras et al ., ). The effective compounds include polyethylene glycol, ginkgo biloba extract, sildenafil, selenium, taurine and Ca 2+ ‐channel blockers, among others.…”

Section: Discussionmentioning

confidence: 99%

“…The protective effects of heparin and its derivatives in IR injury have been evaluated in various systems including hepatic, gastrointestinal, urogenital systems and in pancreas, lungs and heart (Yagmurdur et al ., ; Mocco et al ., ; Taha et al ., ; Gedik et al ., ; Ghadie et al ., ; Warzecha et al ., ). But, no previous study has addressed the effect of heparin in the prevention of IR injury in the testes.…”

Section: Introductionmentioning

confidence: 96%

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Protective role of methylprednisolone and heparin in ischaemic-reperfusion injury of the rat testicle

et al. 2015

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This study evaluated the therapeutic efficacy of heparin and methylprednisolone in the treatment of ischaemic reperfusion (IR) injury of the testis. Twenty-four male Sprague-Dawley rats were allocated equally into three groups of eight animals each. The left testes were rotated 720° for 2 h in the rats in the torsion-detorsion group. Rats in the treatment groups underwent the same surgical procedure as the torsion-detorsion group but were also given methylprednisolone (group II) or heparin (group III) by an intraperitoneal route 30 min prior to detorsion. Left orchiectomy was performed in all rats from each experimental animal at 2 h after detorsion, and the tissue was harvested for the measurement of malondialdehyde (MDA), protein carbonyl (PC) and nitric oxide (NO) and the endogenous antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase. Additional tissue was evaluated using histopathological and immunohistochemical changes. PC and MDA levels were significantly reduced in the treated groups compared to the control group. There was no statistically significant difference in NO level or SOD, GSH-Px and catalase activity among the treatment groups. Histopathological and immunohistochemical findings supported biochemical changes. It is concluded that pre-treatment with methylprednisolone or heparin protects the testis in ischaemic reperfusion injury caused by testicular torsion-detorsion.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Protective role of methylprednisolone and heparin in ischaemic-reperfusion injury of the rat testicle

et al. 2015

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“…Heparin, that is recognised as unfractionated heparin, is a medication that is used as an anticoagulant to treat heart attacks and unstable angina (Boettcher et al, 2016). Previous studies have proved the beneficial impacts of heparin on I/R damage in different body organs such as lungs, heart, liver, pancreas and body systems containing gastrointestinal and urogenital (Gedik et al, 2012;Ghadie et al, 2012;Mocco et al, 2007;Taha et al, 2009;Warzecha et al, 2012;Yagmurdur et al, 2003). Heparin can reduce the accumulation of protein carbonyl and malondialdehyde in the untreated twisted testicle.…”

mentioning

confidence: 99%

The effects of verapamil and heparin co‐administration on sperm parameters and oxidative stress in prevention of testicular torsion/detorsion damage in rats

et al. 2019

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In this research, the impacts of combined administration of verapamil and heparin on testicular torsion damage were examined. In this experimental study, 30 sexually mature male Wistar albino rats were divided into five equal groups haphazardly (n = 6): Group 1 was the sham group. In group 2, a 2‐hr testicular torsion was induced, and thereafter, detorsion was done. Rats in group 3 and group 4 experienced an identical surgical procedure like group 2, but verapamil and heparin were administered in 0.3 mg/kg and 800 IU/kg doses respectively, and in group 5, a combination of verapamil and heparin were administered. Intraperitoneal drug injection in all treatment groups was done 30 min before testicular detorsion. Testicular torsion significantly changed sperm parameters, oxidative stress biomarkers and Cosentino's histological score compared to the sham group (p < .05). All treatment groups reduced testicular damage by decreasing oxidative stress and improving sperm parameters, but heparin and co‐administration of verapamil and heparin were significantly better than verapamil injection alone. However, heparin injected group was more effective than other treatment groups (p < .05). Overall, an anticoagulant like heparin is more effective than a calcium channel blocker such as verapamil, and it is more likely to reduce testicular torsion injuries.

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“…[2][3][4][5][6][7][8][9][10] The molecular disturbance produced in cold-ischemia is caused by the excessive production of pro-inflammatory molecules and free radicals. The injuries caused by ischemia are reflecting upon the mitochondrial activity, decreasing the energetic status for a prolonged time.…”

mentioning

confidence: 99%

Cardioprotective Effects Induced by Preconditioning with Halogenated Anesthetics

Păpurică¹,

Săndesc²,

Rogobete³

et al. 2016

Journal of Interdisciplinary Medicine

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backgroundIn the organ transplantation field, a very intense and important topic is to find ways to increase the quality of organs that are taken from willing donors, in order to improve the recovery capacity of the transplant recipients. 1 From the moment of the organ removal until its actual transplantation, there is a variable period of ischemia, named cold-ischemia, responsible for the production Cardioprotective Effects Induced by Preconditioning with Halogenated AnestheticsMarius Papurica 1,2 , Dorel Sandesc 1,2 , Alexandru Florin Rogobete 1,2 , Radu Nartita 3 , Corina Vernic 2 , Sonia Elena Popovici 2 , Ovidiu Horea Bedreag 1,2 1 Clinic of Anesthesia and Intensive Care, "Pius Brinzeu" Emergency County Hospital, Timișoara, Romania 2 Faculty of Medicine, "Victor Babeș" University of Medicine and Pharmacy, Timișoara, Romania 3 Faculty of Chemistry, Biology, Geography, West University of Timișoara, Romania ABSTRACT Background: Numerous studies discuss the protective effects of halogenated anesthetics on myocyte injury induced by the ischemia-reperfusion syndrome of the heart. This mechanism is known as pharmacological preconditioning. Aim of the study: The objective of this study was to identify the effects of two volatile anesthetics frequently used in current clinical practice, Isoflurane and Sevoflurane, on the in situ heart. The study was performed on laboratory animals with induced brain death. Material and methods: The animals were divided into 3 groups, the control group (n = 8), IZO-PRE group (n = 8) and SEVO-PRE group (n = 8), on which the experimental protocol established for this study was applied. From a molecular point of view, the expressions of protein kinase C-epsilon (PKCε) and of glycogen synthase kinase -3 beta (GSK-3β) were investigated. Results: Following the statistical analysis, we observed a significant reduction in the size of the infarcted area in the IZO-PRE group compared to the control group (p <0.0001). Regarding the SEVO-PRE group, no reduction was observed (p >0.05). The expression of GSK-3β is more pronounced in case of the SEVO-PRE group, at 5 minutes after reperfusion, and the effect disappears after 15 minutes. The expression of PKCε as a total form of the enzyme, occurs at 5 minutes after reperfusion in the SEVO-PRE group and late in the IZO-PRE group (after 15 minutes). Conclusions: Both anesthetics that were applied showed a cardioprotective effect. Isoflurane provided a better structural and morphological protection, but Sevoflurane resulted in a more effective protection in terms of functionality, significantly reducing the incidence of extremely severe life-threatening arrhythmias.

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Study of Heparin in Intestinal Ischemia and Reperfusion in Rats: Morphologic and Functional Evaluation

Cited by 10 publications

References 28 publications

Protective role of methylprednisolone and heparin in ischaemic-reperfusion injury of the rat testicle

Protective role of methylprednisolone and heparin in ischaemic-reperfusion injury of the rat testicle

The effects of verapamil and heparin co‐administration on sperm parameters and oxidative stress in prevention of testicular torsion/detorsion damage in rats

Cardioprotective Effects Induced by Preconditioning with Halogenated Anesthetics

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